通过在喜马拉雅山西北部开展分子和田间研究,评估小麦种质对条锈病和白粉病的双重抗性

IF 1.6 3区 农林科学 Q2 AGRONOMY Euphytica Pub Date : 2024-07-21 DOI:10.1007/s10681-024-03385-4
Shubham Verma, Harinder K. Chaudhary, Anila Badiyal, Kritika Singh, Kulveer Singh Dhillon, Akshay Pathania, Mukul Sharma
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引用次数: 0

摘要

条锈病(SR)和白粉病(PM)等毁灭性病害严重阻碍了喜马拉雅山西北部等凉爽地区的小麦生产。抗条锈病和白粉病的遗传抗性会随着时间的推移而丧失效力,这突出了定期病害筛选的重要性。本研究旨在评估多地 81 个小麦基因型在三年内(2019-20、2021-22 和 2022-23)对 SR 和 PM 的抗性;并使用相应的分子标记,即 SSR/STS引物(STS7/8、Xp3000 和 Xgwm337),检测抗性候选基因(Yr5、Yr10 和 Pm24)。在自然附生条件下,各地分别有 8 个和 12 个基因型表现出对 SR 和 PM 的抗性。值得注意的是,有四个基因型(DH 202、HPW 368、HPW 373 和 DH 114)对这两种病害都具有抗性。分子标记进一步验证了 SR 和 PM 的表型病害反应。基因型 DH 202、DH208、DH 217、CIMMYT Entry no.23 和 VL 829 成为高产抗病基因型。农业气象参数,特别是降水量和相对湿度与病害发生率呈显著正相关,导致谷物减产。基因型和基因型与环境交互作用(GGE)双图谱确定了在不同地点发病率较低的稳定基因型。此外,库库姆塞里可能是筛选抗SR小麦种质的最佳试验地点,而帕兰普尔和库库姆塞里可能是筛选PM的理想地点。对 SR 和 PM 均表现出综合抗病性并具有优良农艺性状的基因型有望立即用作小麦品种,或作为潜在的供体用于培育抗病栽培品种。
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Assessing dual resistance to stripe rust and powdery mildew in wheat germplasm through molecular and field studies across the north-western Himalayas

Wheat production in cooler regions like the north-western Himalayas, is significantly impeded by devastating diseases, namely stripe rust (SR) and powdery mildew (PM). Genetic resistance against SR and PM loses effectiveness over time which underscores the importance of periodic disease screening. This study aims to assess resistance to SR and PM in 81 wheat genotypes across multiple locations over three years (2019–20, 2021–22 and 2022–23); and detect candidate genes (Yr5, Yr10 and Pm24) for resistance using respective molecular markers viz., SSR/STS primers (STS7/8, Xp3000 and Xgwm337). The resistance towards SR and PM under natural epiphytotic conditions was displayed by eight and twelve genotypes respectively, across all locations. Notably, four genotypes (DH 202, HPW 368, HPW 373 and DH 114) were found resistant to both diseases. The phenotypic disease reaction for SR and PM was further validated through molecular markers. Genotypes DH 202, DH208, DH 217, CIMMYT Entry no. 23 and VL 829 emerged as high yielding disease resistant genotypes. Agrometeorological parameters specifically, precipitation and relative humidity exhibited significant positive correlations with disease incidence, leading to reduced grain yields. Genotype and genotype by environment interaction (GGE) biplot identified stable genotypes with less disease incidence over locations. Additionally, Kukumseri may serve as the optimal test site for screening wheat germplasm against SR, while Palampur and Kukumseri could be ideal for PM screening. Genotypes exhibiting combined disease resistance to both SR and PM, alongwith superior agronomic traits, hold promise for immediate deployment as wheat varieties or as potential donors for breeding resistant cultivars.

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来源期刊
Euphytica
Euphytica 农林科学-农艺学
CiteScore
3.80
自引率
5.30%
发文量
157
审稿时长
4.5 months
期刊介绍: Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding. The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.
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