Lisa Leyssens, Noémie Lapraille, Grzegorz Pyka, Pascal J. Jacques, Sandrine Horman, Jeremy Goldman, Greet Kerckhofs
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引用次数: 0
摘要
在使用体内动物模型之前,对用作血管内支架的候选生物可降解金属进行体外测试以评估其降解模式和降解速率至关重要。在这项研究中,我们发现 X 射线微聚焦计算机断层扫描(microCT)在可视化降解模式、评估降解率和材料表面特性方面具有重要的附加值,它能以三维和高分辨率观察大面积的相关区域。我们评估了三种候选生物降解支架材料的体外降解行为:纯铁(Fe)、纯锌(Zn)和二元锌合金(ZnAgCuMnZr)。这些金属与参考的生物稳定钴 铬(CoCr)合金进行了比较。为了比较使用 microCT 评估的降解模式和降解率,我们还使用了扫描电子显微镜(SEM)和电感耦合等离子体(ICP)。我们证实,铁的降解速度非常缓慢,但表面腐蚀非常均匀。锌的降解速度较快,但表现出局部深点蚀。锌合金的降解速度与纯锌相似,但更均匀。不过,也观察到了深层内部树枝状突起的形成。我们的研究以无损方式详细比较了不同候选支架材料的基本表面和腐蚀特性,并对其腐蚀行为进行了三维结构表征。
Exploring the biodegradability of candidate metallic intravascular stent materials using X-ray microfocus computed tomography: An in vitro study
In vitro testing for evaluating degradation mode and rate of candidate biodegradable metals to be used as intravascular stents is crucial before going to in vivo animal models. In this study, we show that X-ray microfocus computed tomography (microCT) presents a key added value to visualize degradation mode and to evaluate degradation rate and material surface properties in 3D and at high resolution of large regions of interest. The in vitro degradation behavior of three candidate biodegradable stent materials was evaluated: pure iron (Fe), pure zinc (Zn), and a quinary Zn alloy (ZnAgCuMnZr). These metals were compared to a reference biostable cobaltchromium (CoCr) alloy. To compare the degradation mode and degradation rate evaluated with microCT, scanning electron microscopy (SEM) and inductively-coupled plasma (ICP) were included. We confirmed that Fe degrades very slowly but with desirable uniform surface corrosion. Zn degrades faster but exhibits localized deep pitting corrosion. The Zn alloy degrades at a similar rate as the pure Zn, but more homogeneously. However, the formation of deep internal dendrites was observed. Our study provides a detailed microCT-based comparison of essential surface and corrosion properties, with a structural characterization of the corrosion behavior, of different candidate stent materials in 3D in a non-destructive way.
期刊介绍:
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats:
• original research reports
• short research and development reports
• scientific reviews
• current concepts articles
• special reports
• editorials
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.