Dan Liang, Rui Yan, Xin Long, Dongmei Ji, Bing Song, Mengyao Wang, Fan Zhang, Xin Cheng, Fengyuan Sun, Ran Zhu, Xinling Hou, Tianjuan Wang, Weiwei Zou, Ying Zhang, Zhixin Pu, Jing Zhang, Zhiguo Zhang, Yajing Liu, Yuqiong Hu, Xiaojin He, Yunxia Cao, Fan Guo
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引用次数: 0
摘要
DNA 5-甲基胞嘧啶(5mC)通过 TET 酶转化为 5-羟甲基胞嘧啶(5hmC)是一种重要的表观遗传修饰,但它在人类早期胚胎中的作用在很大程度上仍不为人所知。在这里,我们发现人类早期胚胎从卵母细胞中继承了大量的 5hmC,而这些卵母细胞在生长过程中意外地发生了新的羟甲基化。此外,受精后父系基因组中 5hmC 的生成大致遵循母系模式,这与 DNA 甲基化动态和持续甲基化区域有关。5hmC一直持续到八细胞阶段,并在OTX2结合位点表现出高度富集,而在人类胚胎中敲除OTX2会影响早期系基因的表达。具体来说,5hmC的耗竭影响了胚胎基因的激活,这一点通过在人类早期胚胎中异位表达小鼠Tet3得到了进一步评估。这些发现揭示了 5hmC 的独特动态,并揭示了它在人类早期胚胎发育中的多方面功能。
Distinct dynamics of parental 5-hydroxymethylcytosine during human preimplantation development regulate early lineage gene expression
The conversion of DNA 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) by TET enzymes represents a significant epigenetic modification, yet its role in early human embryos remains largely unknown. Here we showed that the early human embryo inherited a significant amount of 5hmCs from an oocyte, which unexpectedly underwent de novo hydroxymethylation during its growth. Furthermore, the generation of 5hmC in the paternal genome after fertilization roughly followed the maternal pattern, which was linked to DNA methylation dynamics and regions of sustained methylation. The 5hmCs persisted until the eight-cell stage and exhibited high enrichment at OTX2 binding sites, whereas knockdown of OTX2 in human embryos compromised the expression of early lineage genes. Specifically, the depletion of 5hmC affected the activation of embryonic genes, which was further evaluated by ectopically expressing mouse Tet3 in human early embryos. These findings revealed distinct dynamics of 5hmC and unravelled its multifaceted functions in early human embryonic development. Liang, Yan, Long, Ji et al. find that the origin and dynamics of 5-hydroxymethylcytosine (5hmC) during early development are not conserved between humans and mice and that 5hmC contributes to the activation of human embryonic genes.
期刊介绍:
Nature Cell Biology, a prestigious journal, upholds a commitment to publishing papers of the highest quality across all areas of cell biology, with a particular focus on elucidating mechanisms underlying fundamental cell biological processes. The journal's broad scope encompasses various areas of interest, including but not limited to:
-Autophagy
-Cancer biology
-Cell adhesion and migration
-Cell cycle and growth
-Cell death
-Chromatin and epigenetics
-Cytoskeletal dynamics
-Developmental biology
-DNA replication and repair
-Mechanisms of human disease
-Mechanobiology
-Membrane traffic and dynamics
-Metabolism
-Nuclear organization and dynamics
-Organelle biology
-Proteolysis and quality control
-RNA biology
-Signal transduction
-Stem cell biology