Pub Date : 2026-02-03DOI: 10.1038/s41556-025-01859-8
Daniel Neville, Daniel T. Ferguson, Emily B. Heikamp, Zhihao Lai, Graham W. Magor, Charlene Lam, Olivia G. Dobbs, Vita Levina, Kathy Knezevic, James J. The, Shania Alex, Stephen C. Suits, Bradon Rumler, Michael Uckelmann, Laure Talarmain, Enid Y. N. Lam, Andrew C. Perkins, Scott A. Armstrong, Charles C. Bell, Chen Davidovich, Omer Gilan
DOT1L and Menin are essential cofactors for the oncogenic activity of MLL fusion proteins (MLL-FPs) in leukaemia. However, the mechanisms underpinning the therapeutic effects of their inhibitors remain unclear. Here we identify a critical role for the non-canonical Polycomb repressive complex 1.1 (PRC1.1) in mediating the cellular responses to DOT1L and Menin inhibitors. Menin inhibition induces PRC1.1-dependent deposition of H2AK119ub to silence a subset of MLL-FP targets, whereas DOT1L inhibition results in a genome-wide increase in H2AK119ub. We show that enhanced PRC1.1 activity arises specifically from the progressive loss of DOT1L-mediated H3K79 methylation, independent of MLL-FP displacement or transcriptional repression. This regulatory crosstalk is conserved across cell types and is driven by direct biochemical antagonism between H3K79 methylation and PRC1 activity. Together, our findings establish DOT1L as a component of transcriptional memory co-opted in leukaemia and suggest it serves as the missing link balancing the opposing forces of the MLL–Polycomb axis.
{"title":"DOT1L provides transcriptional memory through PRC1.1 antagonism","authors":"Daniel Neville, Daniel T. Ferguson, Emily B. Heikamp, Zhihao Lai, Graham W. Magor, Charlene Lam, Olivia G. Dobbs, Vita Levina, Kathy Knezevic, James J. The, Shania Alex, Stephen C. Suits, Bradon Rumler, Michael Uckelmann, Laure Talarmain, Enid Y. N. Lam, Andrew C. Perkins, Scott A. Armstrong, Charles C. Bell, Chen Davidovich, Omer Gilan","doi":"10.1038/s41556-025-01859-8","DOIUrl":"https://doi.org/10.1038/s41556-025-01859-8","url":null,"abstract":"DOT1L and Menin are essential cofactors for the oncogenic activity of MLL fusion proteins (MLL-FPs) in leukaemia. However, the mechanisms underpinning the therapeutic effects of their inhibitors remain unclear. Here we identify a critical role for the non-canonical Polycomb repressive complex 1.1 (PRC1.1) in mediating the cellular responses to DOT1L and Menin inhibitors. Menin inhibition induces PRC1.1-dependent deposition of H2AK119ub to silence a subset of MLL-FP targets, whereas DOT1L inhibition results in a genome-wide increase in H2AK119ub. We show that enhanced PRC1.1 activity arises specifically from the progressive loss of DOT1L-mediated H3K79 methylation, independent of MLL-FP displacement or transcriptional repression. This regulatory crosstalk is conserved across cell types and is driven by direct biochemical antagonism between H3K79 methylation and PRC1 activity. Together, our findings establish DOT1L as a component of transcriptional memory co-opted in leukaemia and suggest it serves as the missing link balancing the opposing forces of the MLL–Polycomb axis.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"399 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-02DOI: 10.1038/s41556-025-01864-x
Kalina Lapenta, Güneş Parlakgül, Ana Paula Arruda
{"title":"ER gets out of shape with ageing.","authors":"Kalina Lapenta, Güneş Parlakgül, Ana Paula Arruda","doi":"10.1038/s41556-025-01864-x","DOIUrl":"https://doi.org/10.1038/s41556-025-01864-x","url":null,"abstract":"","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":" ","pages":""},"PeriodicalIF":19.1,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146106302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-02DOI: 10.1038/s41556-025-01860-1
Eric K. F. Donahue, Nathaniel L. Hepowit, Elizabeth M. Ruark, Alexandra G. Mulligan, Brennen Keuchel, Nicholas D. Urban, Li Peng, Stedman Stephens, Derek J. Johnson, Natalie S. Wallace, Lauren P. Jackson, Mark H. Ellisman, Rafael Arrojo e Drigo, Andrew W. Folkmann, Matthias C. Truttmann, Jason A. MacGurn, Kristopher Burkewitz
The endoplasmic reticulum (ER) comprises an array of subdomains, each defined by a characteristic structure and function. Although altered ER processes are linked to age-onset pathogenesis, it is unclear whether shifts in ER structure or dynamics underlie these functional changes. Here we establish ER structural and functional remodelling as a conserved feature of ageing across yeast, Caenorhabditis elegans and mammals. Focusing on C. elegans as the exemplar of metazoan ageing, we reveal striking age-related reductions in ER volume across diverse tissues and a morphological shift from rough sheets to tubular ER. This morphological transition corresponds with large-scale shifts in ER proteome composition from protein synthesis to lipid metabolism, a phenomenon conserved in mammalian tissues. We show that Atg8 and ULK1-dependent ER-phagy drives age-associated ER remodelling through tissue-specific factors, including the previously uncharacterized ER-phagy regulator TMEM-131 and the IRE-1–XBP-1 branch of the unfolded protein response. Providing support for a model where ER remodelling is adaptive, diverse lifespan-extending paradigms downscale and remodel ER morphology throughout life. Furthermore, mTOR-dependent lifespan extension in yeast and worms requires ER-phagy, indicating that ER remodelling is a proactive and protective response during ageing. These results reveal ER-phagy and ER dynamics as pronounced, underappreciated mechanisms of both normal ageing and age-delaying interventions.
{"title":"ER remodelling is a feature of ageing and depends on ER-phagy","authors":"Eric K. F. Donahue, Nathaniel L. Hepowit, Elizabeth M. Ruark, Alexandra G. Mulligan, Brennen Keuchel, Nicholas D. Urban, Li Peng, Stedman Stephens, Derek J. Johnson, Natalie S. Wallace, Lauren P. Jackson, Mark H. Ellisman, Rafael Arrojo e Drigo, Andrew W. Folkmann, Matthias C. Truttmann, Jason A. MacGurn, Kristopher Burkewitz","doi":"10.1038/s41556-025-01860-1","DOIUrl":"https://doi.org/10.1038/s41556-025-01860-1","url":null,"abstract":"The endoplasmic reticulum (ER) comprises an array of subdomains, each defined by a characteristic structure and function. Although altered ER processes are linked to age-onset pathogenesis, it is unclear whether shifts in ER structure or dynamics underlie these functional changes. Here we establish ER structural and functional remodelling as a conserved feature of ageing across yeast, Caenorhabditis elegans and mammals. Focusing on C. elegans as the exemplar of metazoan ageing, we reveal striking age-related reductions in ER volume across diverse tissues and a morphological shift from rough sheets to tubular ER. This morphological transition corresponds with large-scale shifts in ER proteome composition from protein synthesis to lipid metabolism, a phenomenon conserved in mammalian tissues. We show that Atg8 and ULK1-dependent ER-phagy drives age-associated ER remodelling through tissue-specific factors, including the previously uncharacterized ER-phagy regulator TMEM-131 and the IRE-1–XBP-1 branch of the unfolded protein response. Providing support for a model where ER remodelling is adaptive, diverse lifespan-extending paradigms downscale and remodel ER morphology throughout life. Furthermore, mTOR-dependent lifespan extension in yeast and worms requires ER-phagy, indicating that ER remodelling is a proactive and protective response during ageing. These results reveal ER-phagy and ER dynamics as pronounced, underappreciated mechanisms of both normal ageing and age-delaying interventions.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"217 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-23DOI: 10.1038/s41556-025-01858-9
Ali Can Savas, Sergei I Grivennikov
{"title":"Metabolic borders shape immune resistance.","authors":"Ali Can Savas, Sergei I Grivennikov","doi":"10.1038/s41556-025-01858-9","DOIUrl":"https://doi.org/10.1038/s41556-025-01858-9","url":null,"abstract":"","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":" ","pages":""},"PeriodicalIF":19.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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