通过 Dap-Seq 和 WGCNA 分析破译 PmMYB21 在梅花早期开花中的调控机制

Forests Pub Date : 2024-07-25 DOI:10.3390/f15081300
Xi Yuan, Ran He, Hui Zhang, Dongyan Liu, Donghuan Liu, Zhihong Niu, Yu Zhang, Xinli Xia
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引用次数: 0

摘要

梅(Prunus mume Siebold & Zucc)是一种重要的园艺果树,在冬季开花。因此,它的开花过程受到低温条件的挑战。转录因子(TF)MYB21 在调控开花过程中起着关键作用,特别是在花瓣扩展和花丝伸长方面。然而,PmMYB21在梅花中的调控机制仍不清楚。为了培育早花栽培品种,深入了解 PmMYB21 调控基因至关重要。我们采用DNA亲和纯化测序(Dap-seq)来鉴定PmMYB21结合的下游基因。结果显示,启动子区域是 PmMYB21 的主要结合区域,AGTTAGGTARR 基序(motif1)是主要的结合序列类型。我们的分析在启动子区域内发现了 8533 个可能与 PmMYB21 结合的基因,这些基因具有 motif1 序列类型。这些基因参与了对开花至关重要的生物过程。通过加权基因共表达网络分析(WGCNA),进一步完善了候选基因,确定了 PmMYB21 在开花活动中的共表达基因。通过整合 Dap-seq 和 WGCNA 数据,我们将候选基因列表缩小到 54 个,重点关注 4 个 MADS-box 基因和 2 个激素信号转导基因,这些基因对低温条件下的开花过程至关重要。这项研究为了解 PmMYB21 在梅花低温开花调控中的分子基础提供了宝贵的见解,为开发适应早花的新栽培品种铺平了道路。
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Deciphering the Regulatory Mechanism of PmMYB21 in Early Flowering of Prunus mume through Dap-Seq and WGCNA Analysis
Prunus mume Siebold & Zucc (mei) is a horticulturally important fruit tree that undergoes anthesis in winter. Therefore, its flowering process is challenged by low-temperatures conditions. The transcription factor (TF) MYB21 is pivotal in regulating the flowering process, and particularly functions in petal expansion and filament elongation. However, the regulatory mechanism of PmMYB21 in mei remains unknown. To breed early-flowering cultivars, a deeper understanding of PmMYB21-regulated genes is essential. We employed DNA affinity purification sequencing (Dap-seq) to identify downstream genes bound by PmMYB21. The results revealed the promoter region is the primary binding region of PmMYB21, and the AGTTAGGTARR motif (motif1) is the predominant binding sequence type. Our analysis identified 8533 genes that are potentially bound by PmMYB21 with the motif1 sequence type, within the promoter region. These genes are involved in biological processes critical to flowering. Further refinement of candidate genes was achieved through Weighted Gene Co-expression Network Analysis (WGCNA), which identified the co-expressed genes of PmMYB21 during flowering activity. Integrating Dap-seq and WGCNA data, we narrowed down the candidate gene list to 54, with a focus on 4 MADS-box genes and 2 hormone signaling genes that are crucial to the flowering process under low-temperature conditions. This study offers valuable insights into the molecular underpinnings of PmMYB21’s role in the low-temperature flowering regulation of mei, paving the way for the development of new cultivars adapted to early blooming.
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