Pedro Martínez-Rodríguez, Paula Henarejos-Escudero, Samanta Hernández-García, Álvaro Sánchez-Ferrer, Fernando Gandía-Herrero
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引用次数: 0
摘要
研究人员使用 24 种纯分子筛选了植物色素甜菜苷的抗淀粉样蛋白生成特性,这些植物色素甜菜苷可作为抗老年痴呆症的潜在营养保健品。22 种桦木烷在体外减少了淀粉样蛋白的聚集,其中 8 种达到 100%,IC50 值在微摩尔范围内。原子力和透射电子显微镜图像显示了与阿尔茨海默氏症有关的典型纤维,以及甜菜苷如何避免其形成。用秀丽隐杆线虫进行的体内实验证实了摄入后的神经保护作用。吲哚啉-betacyanin是最有效的分子,它显著改善了阿尔茨海默病模型CL2355株的趋化行为。此外,深入的分子对接分析表明,色素与淀粉样肽的 N 端区域相互作用。这项工作是该领域最全面的研究,为将甜菜苷作为营养保健品用于预防阿尔茨海默病提供了体外、体内和硅学方面的证据。
In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
Anti-amyloidogenic properties of plant pigments betalains as potential nutraceuticals against Alzheimer's disease have been screened using 24 pure molecules. Twenty-two betalains reduced amyloid aggregation in vitro, eight of them up to 100%, with IC50 values in the micromolar range. Atomic force and transmission electron microscopy images showed the typical fibrils associated with Alzheimer's disease and how betalains avoid its formation. Neuroprotection after ingestion was supported by in vivo experiments with Caenorhabditis elegans. Indoline-betacyanin was the most effective molecule by significantly improving the chemotactic behavior of the CL2355 strain, a model of Alzheimer's disease. Furthermore, in-depth molecular docking analyses revealed that the pigments interact with the N-terminal region of the amyloid peptide. This work is the most comprehensive study in the field and provides in vitro, in vivo, and in silico evidence for the use of betalains as nutraceuticals of relevance in the prevention of Alzheimer's disease.