Joameson Antunes Lima, Alexandre Pio Viana, Caio Cézar Guedes Correa, Debora Souza Mendes, Eileen Azevedo Santos, Flávia Alves da Silva, Letícia da Silva Araújo, Luis Carlos Loose Coelho, Mariana Zandomênico Mangeiro, Natália Veras Reis, Natan Ramos Cavalcante, Rogério Figueiredo Daher, Thays Correa Costa
{"title":"自花授粉对番石榴近交系遗传多样性的影响","authors":"Joameson Antunes Lima, Alexandre Pio Viana, Caio Cézar Guedes Correa, Debora Souza Mendes, Eileen Azevedo Santos, Flávia Alves da Silva, Letícia da Silva Araújo, Luis Carlos Loose Coelho, Mariana Zandomênico Mangeiro, Natália Veras Reis, Natan Ramos Cavalcante, Rogério Figueiredo Daher, Thays Correa Costa","doi":"10.1007/s10681-024-03389-0","DOIUrl":null,"url":null,"abstract":"<p>In guava (<i>Psidium guajava</i>), the impact of self-pollination on the quantitative traits of the fruits is not fully understood, necessitating further investigation. This study aimed to estimate the effects of selfing on fruit traits in S<sub>1</sub> and S<sub>2</sub> inbred families of guava and to explore potential impacts on genetic diversity. Eighteen S<sub>1</sub> families were generated through selfing of progenies from biparental crosses, and ten S<sub>2</sub> families were produced by selfing superior genotypes from S<sub>1</sub> families. The experiment was conducted at the Experimental Station of Ilha Barra do Pomba, in the municipality of Itaocara-Rio de Janeiro, Brazil. It utilized a randomized complete block design, with three replications and ten plants per plot. Evaluated traits included fruit weight, length, diameter, length-diameter ratio, endocarp thickness, mesocarp thickness, pulp weight, and soluble solids content. The data underwent individual analysis of variance, yielding predicted mean trait values for S<sub>1</sub> and S<sub>2</sub> generations, alongside correlation and homozygosity estimates. Genetic diversity was assessed using Mahalanobis distance and UPGMA cluster analysis, and comparative box plots between inbred populations were created for the evaluated traits. Box plot analysis revealed symmetry in most evaluated traits, suggesting uniformity in the data due to the selfing strategy. Analysis of variance indicated statistically significant differences in all traits, highlighting variability between populations S<sub>1</sub> and S<sub>2</sub>. Fruit and pulp weights exhibited high homozygosity levels, with values of 90.86 and 102.59 respectively, linked to increased fruit traits in the S<sub>2</sub> population, indicating their importance in the fixation of favorable alleles. Fruit weight, length, and diameter, endocarp thickness, and mesocarp thickness showed strong correlations, exceeding 0.70. Genetic diversity assessment via Mahalanobis distance indicated a decrease in genetic variability, evidenced by fewer groups in S<sub>2</sub> compared to the S<sub>1</sub> population. However, this reduction did not noticeably affect the average performance of the S<sub>2</sub> population. The results indicate that the two generations of self-pollination did not negatively affect the phenotypic values of the evaluated traits.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of self-pollination on the genetic diversity of inbred families of Psidium guajava L\",\"authors\":\"Joameson Antunes Lima, Alexandre Pio Viana, Caio Cézar Guedes Correa, Debora Souza Mendes, Eileen Azevedo Santos, Flávia Alves da Silva, Letícia da Silva Araújo, Luis Carlos Loose Coelho, Mariana Zandomênico Mangeiro, Natália Veras Reis, Natan Ramos Cavalcante, Rogério Figueiredo Daher, Thays Correa Costa\",\"doi\":\"10.1007/s10681-024-03389-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In guava (<i>Psidium guajava</i>), the impact of self-pollination on the quantitative traits of the fruits is not fully understood, necessitating further investigation. This study aimed to estimate the effects of selfing on fruit traits in S<sub>1</sub> and S<sub>2</sub> inbred families of guava and to explore potential impacts on genetic diversity. Eighteen S<sub>1</sub> families were generated through selfing of progenies from biparental crosses, and ten S<sub>2</sub> families were produced by selfing superior genotypes from S<sub>1</sub> families. The experiment was conducted at the Experimental Station of Ilha Barra do Pomba, in the municipality of Itaocara-Rio de Janeiro, Brazil. It utilized a randomized complete block design, with three replications and ten plants per plot. Evaluated traits included fruit weight, length, diameter, length-diameter ratio, endocarp thickness, mesocarp thickness, pulp weight, and soluble solids content. The data underwent individual analysis of variance, yielding predicted mean trait values for S<sub>1</sub> and S<sub>2</sub> generations, alongside correlation and homozygosity estimates. Genetic diversity was assessed using Mahalanobis distance and UPGMA cluster analysis, and comparative box plots between inbred populations were created for the evaluated traits. Box plot analysis revealed symmetry in most evaluated traits, suggesting uniformity in the data due to the selfing strategy. Analysis of variance indicated statistically significant differences in all traits, highlighting variability between populations S<sub>1</sub> and S<sub>2</sub>. Fruit and pulp weights exhibited high homozygosity levels, with values of 90.86 and 102.59 respectively, linked to increased fruit traits in the S<sub>2</sub> population, indicating their importance in the fixation of favorable alleles. Fruit weight, length, and diameter, endocarp thickness, and mesocarp thickness showed strong correlations, exceeding 0.70. Genetic diversity assessment via Mahalanobis distance indicated a decrease in genetic variability, evidenced by fewer groups in S<sub>2</sub> compared to the S<sub>1</sub> population. However, this reduction did not noticeably affect the average performance of the S<sub>2</sub> population. The results indicate that the two generations of self-pollination did not negatively affect the phenotypic values of the evaluated traits.</p>\",\"PeriodicalId\":11803,\"journal\":{\"name\":\"Euphytica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-07-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Euphytica\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10681-024-03389-0\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Euphytica","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10681-024-03389-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Impact of self-pollination on the genetic diversity of inbred families of Psidium guajava L
In guava (Psidium guajava), the impact of self-pollination on the quantitative traits of the fruits is not fully understood, necessitating further investigation. This study aimed to estimate the effects of selfing on fruit traits in S1 and S2 inbred families of guava and to explore potential impacts on genetic diversity. Eighteen S1 families were generated through selfing of progenies from biparental crosses, and ten S2 families were produced by selfing superior genotypes from S1 families. The experiment was conducted at the Experimental Station of Ilha Barra do Pomba, in the municipality of Itaocara-Rio de Janeiro, Brazil. It utilized a randomized complete block design, with three replications and ten plants per plot. Evaluated traits included fruit weight, length, diameter, length-diameter ratio, endocarp thickness, mesocarp thickness, pulp weight, and soluble solids content. The data underwent individual analysis of variance, yielding predicted mean trait values for S1 and S2 generations, alongside correlation and homozygosity estimates. Genetic diversity was assessed using Mahalanobis distance and UPGMA cluster analysis, and comparative box plots between inbred populations were created for the evaluated traits. Box plot analysis revealed symmetry in most evaluated traits, suggesting uniformity in the data due to the selfing strategy. Analysis of variance indicated statistically significant differences in all traits, highlighting variability between populations S1 and S2. Fruit and pulp weights exhibited high homozygosity levels, with values of 90.86 and 102.59 respectively, linked to increased fruit traits in the S2 population, indicating their importance in the fixation of favorable alleles. Fruit weight, length, and diameter, endocarp thickness, and mesocarp thickness showed strong correlations, exceeding 0.70. Genetic diversity assessment via Mahalanobis distance indicated a decrease in genetic variability, evidenced by fewer groups in S2 compared to the S1 population. However, this reduction did not noticeably affect the average performance of the S2 population. The results indicate that the two generations of self-pollination did not negatively affect the phenotypic values of the evaluated traits.
期刊介绍:
Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding.
The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.