{"title":"冷常压等离子体在面包中的体外生物可及性、对肝细胞的细胞毒性和黄曲霉毒素 B1 的降解模型","authors":"Leila Tabatabaei-Moradi, Anousheh Sharifan, Kobra Hajizadeh, Hossein Bakhoda","doi":"10.1007/s11947-024-03532-8","DOIUrl":null,"url":null,"abstract":"<p>This study aimed to investigate the aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) degradation as a result of using cold plasma by dielectric barrier discharge method at atmospheric pressure with gases (air, nitrogen, argon, and nitrogen + argon) at different times (0, 5, 15, 25, and 35 min) and also to model AFB<sub>1</sub> inactivation, cytotoxicity against human hepatocellular carcinoma cells (Hep-G<sub>2</sub>), and AFB<sub>1</sub> bioaccessibility in simulated gastrointestinal conditions. The plasma gas flow rate was set to 10 L/min, and the length of the plasma jet was set to about 3 cm. AFB<sub>1</sub> was measured in inoculated bread samples by high-performance liquid chromatography (HPLC), and rapid molecular detection of <i>Aspergillus flavus</i> (<i>A. flavus</i>) based on the aflatoxin biosynthesis polymerase chain reaction (PCR) was done. The results showed that by increasing the plasma induction time to 25 min, the AFB<sub>1</sub> degradation increased significantly (<i>p</i> < 0.05) compared to the control sample. Applying different plasmas showed that air had the most effect and argon had the most minor impact on the AFB<sub>1</sub> degradation. The optimized PCR provided a very high specificity to identify and confirm the presence of <i>A. flavus</i>. AFB1 degradation kinetics modeling and model validation revealed that the Weibull model is the best model to describe aflatoxin degradation in inoculated bread. Evaluation of cytotoxicity against Hep-G<sub>2</sub> and bioaccessibility of AFB1 showed that bread samples treated with plasma had lower cytotoxicity and bioaccessibility compared to the control sample. Using air plasma in 25 min more effectively reduced the bioaccessibility and cytotoxicity against Hep-G<sub>2</sub> compared to other plasmas.</p>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Bioaccessibility, Cytotoxicity Against Liver Cells and Degradation Modeling Aflatoxin B1 in Bread by Cold Atmospheric Pressure Plasma\",\"authors\":\"Leila Tabatabaei-Moradi, Anousheh Sharifan, Kobra Hajizadeh, Hossein Bakhoda\",\"doi\":\"10.1007/s11947-024-03532-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study aimed to investigate the aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) degradation as a result of using cold plasma by dielectric barrier discharge method at atmospheric pressure with gases (air, nitrogen, argon, and nitrogen + argon) at different times (0, 5, 15, 25, and 35 min) and also to model AFB<sub>1</sub> inactivation, cytotoxicity against human hepatocellular carcinoma cells (Hep-G<sub>2</sub>), and AFB<sub>1</sub> bioaccessibility in simulated gastrointestinal conditions. The plasma gas flow rate was set to 10 L/min, and the length of the plasma jet was set to about 3 cm. AFB<sub>1</sub> was measured in inoculated bread samples by high-performance liquid chromatography (HPLC), and rapid molecular detection of <i>Aspergillus flavus</i> (<i>A. flavus</i>) based on the aflatoxin biosynthesis polymerase chain reaction (PCR) was done. The results showed that by increasing the plasma induction time to 25 min, the AFB<sub>1</sub> degradation increased significantly (<i>p</i> < 0.05) compared to the control sample. Applying different plasmas showed that air had the most effect and argon had the most minor impact on the AFB<sub>1</sub> degradation. The optimized PCR provided a very high specificity to identify and confirm the presence of <i>A. flavus</i>. AFB1 degradation kinetics modeling and model validation revealed that the Weibull model is the best model to describe aflatoxin degradation in inoculated bread. Evaluation of cytotoxicity against Hep-G<sub>2</sub> and bioaccessibility of AFB1 showed that bread samples treated with plasma had lower cytotoxicity and bioaccessibility compared to the control sample. Using air plasma in 25 min more effectively reduced the bioaccessibility and cytotoxicity against Hep-G<sub>2</sub> compared to other plasmas.</p>\",\"PeriodicalId\":562,\"journal\":{\"name\":\"Food and Bioprocess Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food and Bioprocess Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11947-024-03532-8\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioprocess Technology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11947-024-03532-8","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
In Vitro Bioaccessibility, Cytotoxicity Against Liver Cells and Degradation Modeling Aflatoxin B1 in Bread by Cold Atmospheric Pressure Plasma
This study aimed to investigate the aflatoxin B1 (AFB1) degradation as a result of using cold plasma by dielectric barrier discharge method at atmospheric pressure with gases (air, nitrogen, argon, and nitrogen + argon) at different times (0, 5, 15, 25, and 35 min) and also to model AFB1 inactivation, cytotoxicity against human hepatocellular carcinoma cells (Hep-G2), and AFB1 bioaccessibility in simulated gastrointestinal conditions. The plasma gas flow rate was set to 10 L/min, and the length of the plasma jet was set to about 3 cm. AFB1 was measured in inoculated bread samples by high-performance liquid chromatography (HPLC), and rapid molecular detection of Aspergillus flavus (A. flavus) based on the aflatoxin biosynthesis polymerase chain reaction (PCR) was done. The results showed that by increasing the plasma induction time to 25 min, the AFB1 degradation increased significantly (p < 0.05) compared to the control sample. Applying different plasmas showed that air had the most effect and argon had the most minor impact on the AFB1 degradation. The optimized PCR provided a very high specificity to identify and confirm the presence of A. flavus. AFB1 degradation kinetics modeling and model validation revealed that the Weibull model is the best model to describe aflatoxin degradation in inoculated bread. Evaluation of cytotoxicity against Hep-G2 and bioaccessibility of AFB1 showed that bread samples treated with plasma had lower cytotoxicity and bioaccessibility compared to the control sample. Using air plasma in 25 min more effectively reduced the bioaccessibility and cytotoxicity against Hep-G2 compared to other plasmas.
期刊介绍：
Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community.
The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.
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