氟硅唑通过细胞凋亡和氧化应激诱导斑马鱼的发育毒性、神经毒性和心血管毒性。

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-08-04 DOI:10.1016/j.cbpc.2024.109993
Hojun Lee , Garam An , Whasun Lim , Gwonhwa Song
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引用次数: 0

摘要

氟硅唑是一种著名的三唑类杀菌剂,广泛应用于世界各地的各种作物和水果。环境中经常检测到氟硅唑残留,许多研究人员也报道了氟硅唑对非靶标生物的危害,但氟硅唑的发育毒性尚未完全阐明。本研究调查了氟硅唑诱导斑马鱼发育缺陷的情况,斑马鱼在毒理学研究中被用来评估化学物质对水生物种或脊椎动物的毒性影响。我们证实,接触氟硅唑会影响斑马鱼幼体的存活率和孵化率,并导致形态缺陷、体长缩短、眼睛和头部变小以及心包水肿。此外,还观察到细胞凋亡、氧化应激和炎症。这些因素打断了早期发育阶段正常器官的形成,转基因模型被用来鉴定器官缺陷。我们利用 olig2:dsRed 转基因斑马鱼证实了氟硅唑对神经系统的影响,并利用 cmlc2:dsRed 和 fli1:eGFP 转基因斑马鱼证实了氟硅唑对心血管系统的影响。我们的研究结果证明了氟硅唑对斑马鱼发育的毒性及其机制,以及氟硅唑的有害影响。
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Flusilazole induced developmental toxicity, neurotoxicity, and cardiovascular toxicity via apoptosis and oxidative stress in zebrafish

Flusilazole is a well-known triazole fungicide applied to various crops and fruits worldwide. Flusilazole residues are frequently detected in the environment, and many researchers have reported the hazardous effects of flusilazole on non-target organisms; however, the developmental toxicity of flusilazole has not been fully elucidated. In this study, we investigated flusilazole-induced developmental defects in zebrafish, which are used in toxicology studies to assess the toxic effects of chemicals on aquatic species or vertebrates. We confirmed that flusilazole exposure affected the viability and hatching rate of zebrafish larvae, and resulted in morphological defects, reduced body length, diminished eye and head sizes, and inflated pericardial edema. Apoptosis, oxidative stress, and inflammation were also observed. These factors interrupted the normal organ formation during early developmental stages, and transgenic models were used to identify organ defects. We confirmed the effects of flusilazole on the nervous system using olig2:dsRed transgenic zebrafish, and on the cardiovascular system using cmlc2:dsRed and fli1:eGFP transgenic zebrafish. Our results demonstrate the developmental toxicity of flusilazole and its mechanisms in zebrafish as well as the detrimental effects of flusilazole.

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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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