Ji Chen, Anqi Chen, Ruiyang Tao, Ruxin Zhu, Han Zhang, Xuechun You, Chengtao Li, Suhua Zhang
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引用次数: 0
摘要
DNA 混合物分析是法医遗传学的一项重大挑战,尤其是在处理降解和痕量 DNA 样本时。多SNPs(MNPs)是一种遗传标记,与微单型相似,但分子尺寸较小(- 6 (99.999159%))。这一证据与嫌疑人的陈述相矛盾,有助于案件的侦破。我们的研究结果表明,在法医调查中,MNP 分析在检测降解和痕量 DNA 混合物方面具有巨大潜力。
Solution to a case involving the interpretation of trace degraded DNA mixtures.
DNA mixture analysis poses a significant challenge in forensic genetics, particularly when dealing with degraded and trace amount DNA samples. Multi-SNPs (MNPs) are genetic markers similar to microhaplotypes but with smaller molecular sizes (< 75 bp), making them theoretically more suitable for analyzing degraded and trace amount samples. In this case report, we investigated a cold case involving a campstool stored for over a decade, aiming to detect and locate the suspect's DNA. We employed both conventional capillary electrophoresis-based short tandem repeat (CE-STR) analysis and next-generation sequencing-based multi-SNP (NGS-MNP) analysis. The typing results and deconvolution of the mixed CE-STR profiles were inconclusive regarding the presence of the suspect's DNA in the mixed samples. However, through NGS-MNP analysis and presence probability calculations, we determined that the suspect's DNA was present in the samples from Sect. 4-1 with a probability of 1-8.41 × 10- 6 (99.999159%). This evidence contradicted the suspect's statement and aided in resolving the case. Our findings demonstrate the significant potential of MNP analysis for examining degraded and trace amount DNA mixtures in forensic investigations.
期刊介绍:
The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.