{"title":"利用杂叶蒿叶提取物生物制造银纳米粒子:其抗菌、抗生物膜和抗氧化活性的表征与评估","authors":"","doi":"10.1016/j.jcrysgro.2024.127827","DOIUrl":null,"url":null,"abstract":"<div><p>The escalating occurrence of antibiotic resistance has focused scientific investigation on the creation of alternative treatments. In this work, an environmentally benign and sustainable method of producing silver nanoparticles (AgNPs) utilizing an aqueous extract from <em>Artocarpus heterophyllus</em> leaves is reported. The study aimed to evaluate the antibacterial and antibiofilm properties of AgNPs against a range of pathogens, specifically Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), <em>Acinetobacter baumannii</em>, <em>Pseudomonas aeruginosa</em>, and <em>Klebsiella pneumoniae</em>. The antioxidant activity of these AgNPs was also determined.</p></div><div><h3>Methods</h3><p>The biosynthesis of silver nanoparticles was confirmed and characterized using a range of analytical methods. These methods included UV–visible spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, Laser Particle Sizer, Field emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), and X-ray diffraction (XRD) analysis. Broth microdilution method was used to assess their antibacterial activity. A free radical scavenging assay was used to determine their antioxidant activity.</p></div><div><h3>Results</h3><p>At 471 nm in wavelength, the AgNPs’ peak absorbance was measured. The AgNPs had an average size of 97.3 nm. AgNPs were found to have a face-centered cubic structure according to XRD measurements. The EDX analysis represented elemental silver (Ag<sup>0</sup>) at a concentration of 87.6 %. The synthesized AgNPs possessed antioxidant activity with IC<sub>50</sub> value of 35.16 μg/mL. The synthesized nanoparticles demonstrated remarkable antibacterial activity against several bacterial strains, as evidenced by their low minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values in the ranges of 62.5 to 125 μg/mL. Furthermore, the AgNPs demonstrated potent anti-biofilm properties, significantly decreasing the formation of biofilms. In addition to these promising antimicrobials, antibiofilm and antioxidant properties.</p></div><div><h3>Conclusions</h3><p>The results of this study indicate that the environmentally friendly AgNPs that were synthesized have considerable potential as antibacterial and antibiofilm agents; they provide a sustainable substitute for addressing the issue of antibiotic resistance. Their possible application in clinical settings calls for further thorough research.</p></div>","PeriodicalId":353,"journal":{"name":"Journal of Crystal Growth","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biofabrication of silver nanoparticles using Artocarpus heterophyllus leaves extract: Characterization and evaluation of its antibacterial, antibiofilm, and antioxidant activities\",\"authors\":\"\",\"doi\":\"10.1016/j.jcrysgro.2024.127827\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The escalating occurrence of antibiotic resistance has focused scientific investigation on the creation of alternative treatments. In this work, an environmentally benign and sustainable method of producing silver nanoparticles (AgNPs) utilizing an aqueous extract from <em>Artocarpus heterophyllus</em> leaves is reported. The study aimed to evaluate the antibacterial and antibiofilm properties of AgNPs against a range of pathogens, specifically Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), <em>Acinetobacter baumannii</em>, <em>Pseudomonas aeruginosa</em>, and <em>Klebsiella pneumoniae</em>. The antioxidant activity of these AgNPs was also determined.</p></div><div><h3>Methods</h3><p>The biosynthesis of silver nanoparticles was confirmed and characterized using a range of analytical methods. These methods included UV–visible spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, Laser Particle Sizer, Field emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), and X-ray diffraction (XRD) analysis. Broth microdilution method was used to assess their antibacterial activity. A free radical scavenging assay was used to determine their antioxidant activity.</p></div><div><h3>Results</h3><p>At 471 nm in wavelength, the AgNPs’ peak absorbance was measured. The AgNPs had an average size of 97.3 nm. AgNPs were found to have a face-centered cubic structure according to XRD measurements. The EDX analysis represented elemental silver (Ag<sup>0</sup>) at a concentration of 87.6 %. The synthesized AgNPs possessed antioxidant activity with IC<sub>50</sub> value of 35.16 μg/mL. The synthesized nanoparticles demonstrated remarkable antibacterial activity against several bacterial strains, as evidenced by their low minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values in the ranges of 62.5 to 125 μg/mL. Furthermore, the AgNPs demonstrated potent anti-biofilm properties, significantly decreasing the formation of biofilms. In addition to these promising antimicrobials, antibiofilm and antioxidant properties.</p></div><div><h3>Conclusions</h3><p>The results of this study indicate that the environmentally friendly AgNPs that were synthesized have considerable potential as antibacterial and antibiofilm agents; they provide a sustainable substitute for addressing the issue of antibiotic resistance. Their possible application in clinical settings calls for further thorough research.</p></div>\",\"PeriodicalId\":353,\"journal\":{\"name\":\"Journal of Crystal Growth\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Crystal Growth\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022024824002628\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CRYSTALLOGRAPHY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Crystal Growth","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022024824002628","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CRYSTALLOGRAPHY","Score":null,"Total":0}
Biofabrication of silver nanoparticles using Artocarpus heterophyllus leaves extract: Characterization and evaluation of its antibacterial, antibiofilm, and antioxidant activities
The escalating occurrence of antibiotic resistance has focused scientific investigation on the creation of alternative treatments. In this work, an environmentally benign and sustainable method of producing silver nanoparticles (AgNPs) utilizing an aqueous extract from Artocarpus heterophyllus leaves is reported. The study aimed to evaluate the antibacterial and antibiofilm properties of AgNPs against a range of pathogens, specifically Methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The antioxidant activity of these AgNPs was also determined.
Methods
The biosynthesis of silver nanoparticles was confirmed and characterized using a range of analytical methods. These methods included UV–visible spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, Laser Particle Sizer, Field emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), and X-ray diffraction (XRD) analysis. Broth microdilution method was used to assess their antibacterial activity. A free radical scavenging assay was used to determine their antioxidant activity.
Results
At 471 nm in wavelength, the AgNPs’ peak absorbance was measured. The AgNPs had an average size of 97.3 nm. AgNPs were found to have a face-centered cubic structure according to XRD measurements. The EDX analysis represented elemental silver (Ag0) at a concentration of 87.6 %. The synthesized AgNPs possessed antioxidant activity with IC50 value of 35.16 μg/mL. The synthesized nanoparticles demonstrated remarkable antibacterial activity against several bacterial strains, as evidenced by their low minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values in the ranges of 62.5 to 125 μg/mL. Furthermore, the AgNPs demonstrated potent anti-biofilm properties, significantly decreasing the formation of biofilms. In addition to these promising antimicrobials, antibiofilm and antioxidant properties.
Conclusions
The results of this study indicate that the environmentally friendly AgNPs that were synthesized have considerable potential as antibacterial and antibiofilm agents; they provide a sustainable substitute for addressing the issue of antibiotic resistance. Their possible application in clinical settings calls for further thorough research.
期刊介绍:
The journal offers a common reference and publication source for workers engaged in research on the experimental and theoretical aspects of crystal growth and its applications, e.g. in devices. Experimental and theoretical contributions are published in the following fields: theory of nucleation and growth, molecular kinetics and transport phenomena, crystallization in viscous media such as polymers and glasses; crystal growth of metals, minerals, semiconductors, superconductors, magnetics, inorganic, organic and biological substances in bulk or as thin films; molecular beam epitaxy, chemical vapor deposition, growth of III-V and II-VI and other semiconductors; characterization of single crystals by physical and chemical methods; apparatus, instrumentation and techniques for crystal growth, and purification methods; multilayer heterostructures and their characterisation with an emphasis on crystal growth and epitaxial aspects of electronic materials. A special feature of the journal is the periodic inclusion of proceedings of symposia and conferences on relevant aspects of crystal growth.