杂交捕获解决了四叶草(芸香科)的系统发育问题,并支持将苏拉威西的一个物种纳入其中

IF 3.7 1区 生物学 Q1 Agricultural and Biological Sciences Journal of Systematics and Evolution Pub Date : 2024-08-05 DOI:10.1111/jse.13127
Lee‐Ping Ang, Fabian Brambach, Salvatore Tomasello, Jun Wen, Marc S. Appelhans
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引用次数: 0

摘要

Tetradium 是芸香科的一个属,共有九种,分布于亚洲东部和东南部,其特征是羽状复叶对生,雌蕊无果或近无果,有蓇葖果。哈特利在 1981 年出版的专著为该属提供了奠基性的见解,但目前还缺乏对该属的全面系统发育分析。本研究利用新一代测序技术(NGS),旨在(i)建立四叶草的 NGS 分子数据集;(ii)通过杂交捕获法阐明种间关系;(iii)研究 Euodia meliifolia var.我们的数据集包括 9 个物种的 28 个样本,使用 Illumina Miseq 和 Hiseq 4000 平台进行测序,并使用 HybPhyloMaker 管道和 ASTRAL 进行下游分析。我们的发现揭示了分子和形态学数据支持的五个主要群体,突出了胚珠数量和种子功能的变化。值得注意的是,杂交捕获法被证明对研究古老的标本馆标本非常有价值。最后,我们提出了分类修订建议,包括将 E. meliifolia var.celebicum 的最新描述,并补充了具体的鉴定关键字。
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Hybrid capture resolves the phylogeny of Tetradium (Rutaceae) and supports the inclusion of a species from Sulawesi
Tetradium, a genus within Rutaceae, comprises nine species found in Eastern and Southeastern Asia, distinguished by their opposite pinnate leaves and apocarpous or subapocarpous gynoecium with follicular fruits. While Hartley's 1981 monograph provided foundational insights, a comprehensive phylogenetic analysis of the genus is lacking. Using next‐generation sequencing (NGS), this study aims to (i) establish an NGS molecular data set for Tetradium, (ii) elucidate interspecific relationships via the hybrid capture method and (iii) investigate the taxonomic status of Euodia meliifolia var. celebica. Our data set comprised 28 samples across nine species, sequenced using Illumina Miseq and Hiseq 4000 platforms, with downstream analyses conducted using the HybPhyloMaker pipeline and ASTRAL. Our findings revealed five main groups supported by both molecular and morphological data, highlighting changes in ovule number and seed functionality. Notably, the hybrid capture method proved invaluable for studying old herbarium specimens. Finally, taxonomic revisions were proposed, including the reclassification of E. meliifolia var. celebica as Tetradium celebicum, the fossil Euodia costata as Tetradium costatum, and the fossil Euodia lignata as Tetradium lignatum. An updated description for T. celebicum, supplemented by a specific identification key, is provided.
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来源期刊
Journal of Systematics and Evolution
Journal of Systematics and Evolution Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
7.40
自引率
8.10%
发文量
1368
审稿时长
6-12 weeks
期刊介绍: Journal of Systematics and Evolution (JSE, since 2008; formerly Acta Phytotaxonomica Sinica) is a plant-based international journal newly dedicated to the description and understanding of the biological diversity. It covers: description of new taxa, monographic revision, phylogenetics, molecular evolution and genome evolution, evolutionary developmental biology, evolutionary ecology, population biology, conservation biology, biogeography, paleobiology, evolutionary theories, and related subjects.
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