Tao Liu, Haonan Yu, Jun Qin, Wenjing Shang, Jieyin Chen, Krishna V Subbarao, Xiaoping Hu
{"title":"大丽轮枝菌（Verticillium dahliae）中的一个基因盒 Vd276-280 包含两个影响黑色素化小硬壳形成和毒力的基因。","authors":"Tao Liu, Haonan Yu, Jun Qin, Wenjing Shang, Jieyin Chen, Krishna V Subbarao, Xiaoping Hu","doi":"10.1094/PHYTO-11-23-0426-R","DOIUrl":null,"url":null,"abstract":"<p><p><i>Verticillium dahliae</i> is a soilborne phytopathogenic fungus causing Verticillium wilt on hundreds of plant species. Several sequenced genomes of <i>V. dahliae</i> are available, but functional characterization of most genes has just begun. Based on our previous comparison of the transcriptome from the wild-type and Δ<i>VdCf2</i> strains, a significant upregulation of the gene cassette, <i>Vd276-280</i>, in the Δ<i>VdCf2</i> strain was observed. In this study, the functional characterization of the <i>Vd276-280</i> gene cassette was performed. <i>Agrobacterium</i>-mediated knockout of this gene cassette in <i>V. dahliae</i> significantly inhibited conidiation, melanized microsclerotium formation in the mutant strains, and their virulence towards cotton. Furthermore, deletion of individual genes in the <i>Vd276-280</i> gene cassette identified that the disruption of <i>VDAG_07276</i> and <i>VDAG_07280</i> delayed microsclerotium formation, inhibited conidiation, and reduced virulence towards cotton. Our data suggest that <i>VDAG_07276</i> and <i>VDAG_07280</i> in the <i>Vd276-280</i> gene cassette mainly act as positive regulators of development and virulence in <i>V. dahliae</i>.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A gene cassette <i>Vd276-280</i> in <i>Verticillium dahliae</i> contains two genes that affect melanized microsclerotium formation and virulence.\",\"authors\":\"Tao Liu, Haonan Yu, Jun Qin, Wenjing Shang, Jieyin Chen, Krishna V Subbarao, Xiaoping Hu\",\"doi\":\"10.1094/PHYTO-11-23-0426-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Verticillium dahliae</i> is a soilborne phytopathogenic fungus causing Verticillium wilt on hundreds of plant species. Several sequenced genomes of <i>V. dahliae</i> are available, but functional characterization of most genes has just begun. Based on our previous comparison of the transcriptome from the wild-type and Δ<i>VdCf2</i> strains, a significant upregulation of the gene cassette, <i>Vd276-280</i>, in the Δ<i>VdCf2</i> strain was observed. In this study, the functional characterization of the <i>Vd276-280</i> gene cassette was performed. <i>Agrobacterium</i>-mediated knockout of this gene cassette in <i>V. dahliae</i> significantly inhibited conidiation, melanized microsclerotium formation in the mutant strains, and their virulence towards cotton. Furthermore, deletion of individual genes in the <i>Vd276-280</i> gene cassette identified that the disruption of <i>VDAG_07276</i> and <i>VDAG_07280</i> delayed microsclerotium formation, inhibited conidiation, and reduced virulence towards cotton. Our data suggest that <i>VDAG_07276</i> and <i>VDAG_07280</i> in the <i>Vd276-280</i> gene cassette mainly act as positive regulators of development and virulence in <i>V. dahliae</i>.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-11-23-0426-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-11-23-0426-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
A gene cassette Vd276-280 in Verticillium dahliae contains two genes that affect melanized microsclerotium formation and virulence.
Verticillium dahliae is a soilborne phytopathogenic fungus causing Verticillium wilt on hundreds of plant species. Several sequenced genomes of V. dahliae are available, but functional characterization of most genes has just begun. Based on our previous comparison of the transcriptome from the wild-type and ΔVdCf2 strains, a significant upregulation of the gene cassette, Vd276-280, in the ΔVdCf2 strain was observed. In this study, the functional characterization of the Vd276-280 gene cassette was performed. Agrobacterium-mediated knockout of this gene cassette in V. dahliae significantly inhibited conidiation, melanized microsclerotium formation in the mutant strains, and their virulence towards cotton. Furthermore, deletion of individual genes in the Vd276-280 gene cassette identified that the disruption of VDAG_07276 and VDAG_07280 delayed microsclerotium formation, inhibited conidiation, and reduced virulence towards cotton. Our data suggest that VDAG_07276 and VDAG_07280 in the Vd276-280 gene cassette mainly act as positive regulators of development and virulence in V. dahliae.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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