Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Beatriz Galiano-Cogolludo, Ismael Lamas-Toranzo, Julieta G Hamze, Adolfo Toledano-Díaz, Julián Santiago-Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez
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引用次数: 0
摘要
哺乳动物的第一个细胞系分化产生了内细胞团（ICM）和滋养层（TE）。在小鼠中，TEAD4 是 TE 承诺的主调控因子，因为它调控其他 TE 特异性基因的表达，消减 TEAD4 会阻止囊胚形成，但它在其他哺乳动物中的作用仍不清楚。在此，我们观察到，在两个系统发育相远的物种（牛和兔）中，TEAD4 的消减不会阻碍 TE 分化、囊胚形成和 TE 标记（如 GATA3 和 CDX2）的表达，尽管在牛 TEAD4 KO 囊胚中观察到 ICM 细胞数量减少。牛囊胚中的转录分析表明，虽然 KO 胚胎中与下胚层和 Hippo 信号相关基因的表达趋于减少，但消减对转录没有重大影响。在牛模型中进行了实验，以确定TEAD4是否为孵化后发育所必需。TEAD4 KO 的球形胚胎显示胚盘和 TE 发育正常，但低母细胞迁移率降低。在后期发育阶段（管状胚胎），未观察到 KO 和 WT 胚胎之间的差异。
The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals.
The first lineage differentiation in mammals gives rise to the inner cell mass and the trophectoderm (TE). In mice, TEAD4 is a master regulator of TE commitment, as it regulates the expression of other TE-specific genes and its ablation prevents blastocyst formation, but its role in other mammals remains unclear. Herein, we have observed that TEAD4 ablation in two phylogenetically distant species (bovine and rabbit) does not impede TE differentiation, blastocyst formation and the expression of TE markers, such as GATA3 and CDX2, although a reduced number of cells in the inner cell mass was observed in bovine TEAD4 knockout (KO) blastocysts. Transcriptional analysis in bovine blastocysts revealed no major transcriptional effect of the ablation, although the expression of hypoblast and Hippo signalling-related genes tended to be decreased in KO embryos. Experiments were conducted in the bovine model to determine whether TEAD4 was required for post-hatching development. TEAD4 KO spherical conceptuses showed normal development of the embryonic disc and TE, but hypoblast migration rate was reduced. At later stages of development (tubular conceptuses), no differences were observed between KO and wild-type conceptuses.
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