Annabel Simpson, William Johnston, Miguel Carda-Diéguez, Alex Mira, Chris Easton, Fiona L Henriquez, Shauna Culshaw, Bob T Rosier, Mia Burleigh
{"title":"牙周治疗会导致产生亚硝酸盐的细菌纵向增加。","authors":"Annabel Simpson, William Johnston, Miguel Carda-Diéguez, Alex Mira, Chris Easton, Fiona L Henriquez, Shauna Culshaw, Bob T Rosier, Mia Burleigh","doi":"10.1111/omi.12479","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The oral microbiome-dependent nitrate (NO<sub>3</sub> <sup>-</sup>)-nitrite (NO<sub>2</sub> <sup>-</sup>)-nitric oxide (NO) pathway may help regulate blood pressure. NO<sub>2</sub> <sup>-</sup>-producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO<sub>2</sub> <sup>-</sup>-producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO<sub>2</sub> <sup>-</sup>-producing bacteria and the NO<sub>3</sub> <sup>-</sup>-NO<sub>2</sub> <sup>-</sup>-NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO<sub>2</sub> <sup>-</sup>-producing microbiome and salivary NO<sub>3</sub> <sup>-</sup> and NO<sub>2</sub> <sup>-</sup> levels over time.</p><p><strong>Methods: </strong>The subgingival microbiota of 38 periodontitis patients was analysed before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO<sub>2</sub> <sup>-</sup>-producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO<sub>3</sub> <sup>-</sup> and NO<sub>2</sub> <sup>-</sup> levels using gas-phase chemiluminescence.</p><p><strong>Results: </strong>A significant increase was observed in the relative abundance of NO<sub>2</sub> <sup>-</sup>-producing species between BL and all subsequent timepoints (all p < 0.001). Periodontitis-associated species decreased at all timepoints, relative to BL (all p < 0.02). NO<sub>2</sub> <sup>-</sup>-producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = -0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO<sub>3</sub> <sup>-</sup> and NO<sub>2</sub> <sup>-</sup> over time (all p > 0.05).</p><p><strong>Conclusions: </strong>Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO<sub>2</sub> <sup>-</sup>-producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO<sub>3</sub> <sup>-</sup> intake on periodontal and cardiovascular health.</p>","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":" ","pages":"491-506"},"PeriodicalIF":2.8000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria.\",\"authors\":\"Annabel Simpson, William Johnston, Miguel Carda-Diéguez, Alex Mira, Chris Easton, Fiona L Henriquez, Shauna Culshaw, Bob T Rosier, Mia Burleigh\",\"doi\":\"10.1111/omi.12479\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The oral microbiome-dependent nitrate (NO<sub>3</sub> <sup>-</sup>)-nitrite (NO<sub>2</sub> <sup>-</sup>)-nitric oxide (NO) pathway may help regulate blood pressure. NO<sub>2</sub> <sup>-</sup>-producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO<sub>2</sub> <sup>-</sup>-producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO<sub>2</sub> <sup>-</sup>-producing bacteria and the NO<sub>3</sub> <sup>-</sup>-NO<sub>2</sub> <sup>-</sup>-NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO<sub>2</sub> <sup>-</sup>-producing microbiome and salivary NO<sub>3</sub> <sup>-</sup> and NO<sub>2</sub> <sup>-</sup> levels over time.</p><p><strong>Methods: </strong>The subgingival microbiota of 38 periodontitis patients was analysed before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO<sub>2</sub> <sup>-</sup>-producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO<sub>3</sub> <sup>-</sup> and NO<sub>2</sub> <sup>-</sup> levels using gas-phase chemiluminescence.</p><p><strong>Results: </strong>A significant increase was observed in the relative abundance of NO<sub>2</sub> <sup>-</sup>-producing species between BL and all subsequent timepoints (all p < 0.001). Periodontitis-associated species decreased at all timepoints, relative to BL (all p < 0.02). NO<sub>2</sub> <sup>-</sup>-producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = -0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO<sub>3</sub> <sup>-</sup> and NO<sub>2</sub> <sup>-</sup> over time (all p > 0.05).</p><p><strong>Conclusions: </strong>Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO<sub>2</sub> <sup>-</sup>-producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO<sub>3</sub> <sup>-</sup> intake on periodontal and cardiovascular health.</p>\",\"PeriodicalId\":18815,\"journal\":{\"name\":\"Molecular Oral Microbiology\",\"volume\":\" \",\"pages\":\"491-506\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Oral Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/omi.12479\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Oral Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/omi.12479","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/22 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria.
Background: The oral microbiome-dependent nitrate (NO3-)-nitrite (NO2-)-nitric oxide (NO) pathway may help regulate blood pressure. NO2--producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO2--producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO2--producing bacteria and the NO3--NO2--NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO2--producing microbiome and salivary NO3- and NO2- levels over time.
Methods: The subgingival microbiota of 38 periodontitis patients was analysed before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO2--producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO3- and NO2- levels using gas-phase chemiluminescence.
Results: A significant increase was observed in the relative abundance of NO2--producing species between BL and all subsequent timepoints (all p < 0.001). Periodontitis-associated species decreased at all timepoints, relative to BL (all p < 0.02). NO2--producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = -0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO3- and NO2- over time (all p > 0.05).
Conclusions: Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO2--producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO3- intake on periodontal and cardiovascular health.
期刊介绍：
Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections.
Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal.
The journal does not publish Short Communications or Letters to the Editor.
Molecular Oral Microbiology is published bimonthly.
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