Philipp Schuler, Oliver Rehmann, Valentina Vitali, Matthias Saurer, Manuela Oettli, Lucas A Cernusak, Arthur Gessler, Nina Buchmann, Marco M Lehmann
{"title":"C3、C4 和 CAM CO2 固定植物的氢同位素分馏。","authors":"Philipp Schuler, Oliver Rehmann, Valentina Vitali, Matthias Saurer, Manuela Oettli, Lucas A Cernusak, Arthur Gessler, Nina Buchmann, Marco M Lehmann","doi":"10.1111/nph.20057","DOIUrl":null,"url":null,"abstract":"<p><p>Measurements of stable isotope ratios in organic compounds are widely used tools for plant ecophysiological studies. However, the complexity of the processes involved in shaping hydrogen isotope values (δ<sup>2</sup>H) in plant carbohydrates has limited its broader application. To investigate the underlying biochemical processes responsible for <sup>2</sup>H fractionation among water, sugars, and cellulose in leaves, we studied the three main CO<sub>2</sub> fixation pathways (C<sub>3</sub>, C<sub>4</sub>, and CAM) and their response to changes in temperature and vapor pressure deficit (VPD). We show significant differences in autotrophic <sup>2</sup>H fractionation (ε<sub>A</sub>) from water to sugar among the pathways and their response to changes in air temperature and VPD. The strong <sup>2</sup>H depleting ε<sub>A</sub> in C<sub>3</sub> plants is likely driven by the photosynthetic H<sup>+</sup> production within the thylakoids, a reaction that is spatially separated in C<sub>4</sub> and strongly reduced in CAM plants, leading to the absence of <sup>2</sup>H depletion in the latter two types. By contrast, we found that the heterotrophic <sup>2</sup>H-fractionation (ε<sub>H</sub>) from sugar to cellulose was very similar among the three pathways and is likely driven by the plant's metabolism, rather than by isotopic exchange with leaf water. Our study offers new insights into the biochemical drivers of <sup>2</sup>H fractionation in plant carbohydrates.</p>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hydrogen isotope fractionation in plants with C<sub>3</sub>, C<sub>4</sub>, and CAM CO<sub>2</sub> fixation.\",\"authors\":\"Philipp Schuler, Oliver Rehmann, Valentina Vitali, Matthias Saurer, Manuela Oettli, Lucas A Cernusak, Arthur Gessler, Nina Buchmann, Marco M Lehmann\",\"doi\":\"10.1111/nph.20057\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Measurements of stable isotope ratios in organic compounds are widely used tools for plant ecophysiological studies. However, the complexity of the processes involved in shaping hydrogen isotope values (δ<sup>2</sup>H) in plant carbohydrates has limited its broader application. To investigate the underlying biochemical processes responsible for <sup>2</sup>H fractionation among water, sugars, and cellulose in leaves, we studied the three main CO<sub>2</sub> fixation pathways (C<sub>3</sub>, C<sub>4</sub>, and CAM) and their response to changes in temperature and vapor pressure deficit (VPD). We show significant differences in autotrophic <sup>2</sup>H fractionation (ε<sub>A</sub>) from water to sugar among the pathways and their response to changes in air temperature and VPD. The strong <sup>2</sup>H depleting ε<sub>A</sub> in C<sub>3</sub> plants is likely driven by the photosynthetic H<sup>+</sup> production within the thylakoids, a reaction that is spatially separated in C<sub>4</sub> and strongly reduced in CAM plants, leading to the absence of <sup>2</sup>H depletion in the latter two types. By contrast, we found that the heterotrophic <sup>2</sup>H-fractionation (ε<sub>H</sub>) from sugar to cellulose was very similar among the three pathways and is likely driven by the plant's metabolism, rather than by isotopic exchange with leaf water. Our study offers new insights into the biochemical drivers of <sup>2</sup>H fractionation in plant carbohydrates.</p>\",\"PeriodicalId\":48887,\"journal\":{\"name\":\"New Phytologist\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.4000,\"publicationDate\":\"2024-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Phytologist\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/nph.20057\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/nph.20057","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Hydrogen isotope fractionation in plants with C3, C4, and CAM CO2 fixation.
Measurements of stable isotope ratios in organic compounds are widely used tools for plant ecophysiological studies. However, the complexity of the processes involved in shaping hydrogen isotope values (δ2H) in plant carbohydrates has limited its broader application. To investigate the underlying biochemical processes responsible for 2H fractionation among water, sugars, and cellulose in leaves, we studied the three main CO2 fixation pathways (C3, C4, and CAM) and their response to changes in temperature and vapor pressure deficit (VPD). We show significant differences in autotrophic 2H fractionation (εA) from water to sugar among the pathways and their response to changes in air temperature and VPD. The strong 2H depleting εA in C3 plants is likely driven by the photosynthetic H+ production within the thylakoids, a reaction that is spatially separated in C4 and strongly reduced in CAM plants, leading to the absence of 2H depletion in the latter two types. By contrast, we found that the heterotrophic 2H-fractionation (εH) from sugar to cellulose was very similar among the three pathways and is likely driven by the plant's metabolism, rather than by isotopic exchange with leaf water. Our study offers new insights into the biochemical drivers of 2H fractionation in plant carbohydrates.
期刊介绍:
New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.