用于检测淡水和海水中肠球菌的抗干扰金纳米粒子测定法。

Journal of hazardous materials Pub Date : 2024-10-05 Epub Date: 2024-08-09 DOI:10.1016/j.jhazmat.2024.135463
Yunsoo Chang, Ji-Hyeon Sung, Seung-Woo Lee, Eun-Hee Lee
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引用次数: 0

摘要

肠球菌是粪便污染的常见指标,用于评估淡水和海水、沙子、土壤和沉积物的质量。然而,从这些环境中采集的样本含有各种细胞和其他因素,会干扰用于检测肠球菌的检测方法。我们开发了一种新型检测方法,用于灵敏、特异地检测肠球菌,该方法可抵抗其他细胞和环境因素的干扰。我们的抗干扰测定使用了 30 纳米金颗粒 (AuNPs)、链霉亲和素和生物素化的肠球菌抗体。肠球菌抑制了链霉亲和素和生物素之间的相互作用,导致 AuNPs 分解。没有肠球菌则会导致 AuNPs 聚合,这种差异很容易通过分光光度法检测出来。这种抗干扰 AuNP 检测法能够在 3 小时内检测到 10 至 107 CFU/mL 的肠球菌全细胞,对肠球菌具有高度特异性,并且不受大肠杆菌等其他肠道细菌存在的影响。我们对淡水和海水样本的检测结果表明,没有受到其他细胞或环境因素的干扰。本文介绍的抗干扰 AuNP 检测法有望成为一种快速、简单、有效的方法,用于监测各种环境样本中的肠球菌。
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Interference-resistant gold nanoparticle assay for detecting Enterococcus in fresh and marine waters.

Enterococci are common indicators of fecal contamination and are used to assess the quality of fresh and marine water, sand, soil, and sediment. However, samples collected from these environments contain various cells and other factors that can interfere with the assays used to detect enterococci. We developed a novel assay for the sensitive and specific detection of enterococci that is resistant to interference from other cells and environmental factors. Our interference-resistant assay used 30-nm gold nanoparticles (AuNPs), streptavidin, and a biotinylated Enterococcus antibody. Enterococci inhibited the interaction between streptavidin and biotin and led to the disaggregation of AuNPs. The absence of enterococci led to the aggregation of AuNPs, and this difference was easily detected by spectrophotometry. This interference-resistant AuNP assay was able to detect whole cells of Enterococcus in the range of 10 to 107 CFU/mL within 3 h, had high specificity for enterococci, and was unaffected by the presence of other intestinal bacteria, such as Escherichia coli. Our examination of fresh and marine water samples demonstrated no interference from other cells or environmental factors. The interference-resistant AuNP assay described here has the potential to be used as a rapid, simple, and effective method for monitoring enterococci in diverse environmental samples.

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