用蚕丝脱胶水中的酶水解物合成黑色素样氨基酸表面活性剂。

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of biotechnology Pub Date : 2024-08-22 DOI:10.1016/j.jbiotec.2024.08.011
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引用次数: 0

摘要

丝绸加工过程中产生的脱胶废水含有大量的氨基酸和丝胶多肽。丝绸脱胶水远未得到充分利用。脱胶水中的丝胶蛋白一般都被浪费掉,造成环境污染。本研究利用碱性蛋白酶水解模拟丝胶脱胶水,以产生丰富的氨基酸和多肽。酶水解后,蚕丝脱胶水中游离氨基酸的最大浓度约为 54 mM。这有助于丝绸脱胶水的循环利用,以生产黑色素类氨基酸表面活性剂为原料。以 4-叔丁基邻苯二酚为起始原料,经硝酸铈铵氧化生成邻醌,邻醌与丝胶脱胶水酶水解产物中的游离氨基偶联,分别作为疏水基团和亲水基团合成丝胶素类氨基酸表面活性剂。通过绿色简单的合成路线,该产品具有新颖的类黑色素结构。该产品具有优异的表面活性特性,可将表面张力降至 32.39 mN m-1。此外,它还具有良好的发泡能力和泡沫稳定性,初始泡沫量为 37 mL,泡沫半衰期超过 25 分钟。该产品在油水乳液中具有良好的乳化能力,大豆油和液体石蜡形成的乳液的分层时间分别为 297 秒和 291 秒。帆布片的润湿时间仅为 134 秒。因此,该产品具有低表面张力、良好的发泡、乳化和润湿性能。
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Synthesis of melanin-like amino acid surfactant with enzymatic hydrolysates from silk degumming water

The degummed wastewater from silk processing contains a huge amount of amino acids and polypeptides from sericin. The silk degumming water is far from being exploited fully. Sericin in the degumming water is generally wasted and causes environmental pollution. In this study, simulated silk degumming water was hydrolyzed by alkaline protease to produce abundant amino acids and polypeptides. After enzymatic hydrolysis, the maximum free amino groups concentration in the silk degumming water was approximately 54 mM. It facilitated the recycling of silk degumming water for the production of melanin-like amino acid surfactants as raw materials. 4-Tert-butylcatechol was used as the starting material to generate o-quinone via oxidation by ceric ammonium nitrate. o-Quinone was coupled with free amino groups in enzymatic hydrolysates of silk degumming water to synthesize a sericin-based amino acid surfactant as hydrophobic and hydrophilic group, respectively. Through the green and simple synthesis route, the product was characterized to have a novel melanin-like structure. The product exhibited superior surface-active properties by lowering the surface tension to 32.39 mN m−1. Furthermore, it demonstrated good foaming ability and foam stability, with the initial foam volume of 37 mL and the foam half-life time of more than 25 min. The product owned a good emulsification ability in the oil-water emulsion with delamination time of 297 s and 291 s for emulsion formed by soybean oil and liquid paraffin, respectively. The wetting time of the canvas sheet was only 134 s. Consequently, the product showed low surface tension, good foaming, emulsifying, and wetting properties.

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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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