在稻瘟病中，SNARE 复合物对 retromer 的空泡招募实现了与感染相关的迁移。

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-08-23 DOI:10.1111/nph.20069

Xin Chen, Jiexiong Hu, Haoming Zhong, Qiuqiu Wu, Zhenyu Fang, Yan Cai, Panpan Huang, Yakubu Saddeeq Abubakar, Jie Zhou, Naweed I. Naqvi, Zonghua Wang, Wenhui Zheng

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引用次数: 0

摘要

retromer 复合物是一种保守的分拣机制，它通过将含有货物蛋白质的囊泡运输到确定的目的地来维持细胞蛋白质的平衡。众所周知，它能在液泡膜上分拣蛋白质进行逆向运输，防止其在液泡中降解。然而，retromer 招募到液泡膜的详细机制尚未阐明。在这里，我们发现液泡SNARE复合体MoPep12-MoVti1-MoVam7-MoYkt6通过将retromer招募到液泡膜上来调节retromer介导的囊泡贩运，从而促进了木格氏球菌的宿主入侵。这种募集对于自噬调节因子 MoAtg8 的回收也是必不可少的，并能实现由附属物介导的宿主渗透。此外，液泡 SNARE 亚基通过调节 retromer-MoSnc1 介导的效应物分泌的部署，参与抑制宿主的防御反应。总之，我们的研究结果提供了对依赖于液泡 SNARE 的 retromer 招募机制的见解，这种招募是稻瘟病真菌中与致病性相关的膜贩运事件所必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Vacuolar recruitment of retromer by a SNARE complex enables infection-related trafficking in rice blast

The retromer complex is a conserved sorting machinery that maintains cellular protein homeostasis by transporting vesicles containing cargo proteins to defined destinations. It is known to sort proteins at the vacuole membranes for retrograde trafficking, preventing their degradation in the vacuole. However, the detailed mechanism of retromer recruitment to the vacuole membrane has not yet been elucidated.
Here, we show that the vacuolar SNARE complex MoPep12-MoVti1-MoVam7-MoYkt6 regulates retromer-mediated vesicle trafficking by recruiting the retromer to the vacuole membrane, which promotes host invasion in Magnaporthe oryzae.
Such recruitment is also essential for the retrieval of the autophagy regulator MoAtg8 and enables appressorium-mediated host penetration. Furthermore, the vacuolar SNARE subunits are involved in suppressing the host defense response by regulating the deployment of retromer-MoSnc1-mediated effector secretion.
Altogether, our results provide insights into the mechanism of vacuolar SNAREs-dependent retromer recruitment which is necessary for pathogenicity-related membrane trafficking events in the rice blast fungus.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.