玫瑰中挥发性萜烯生物合成的复杂性:对 β-Citronellol 生产的特殊见解。

IF 6.5 1区 生物学 Q1 PLANT SCIENCES Plant Physiology Pub Date : 2024-11-04 DOI:10.1093/plphys/kiae444
Hongjie Li, Yueqing Li, Huijun Yan, Tingting Bao, Xiaotong Shan, Jean-Claude Caissard, Liangsheng Zhang, Huiyi Fang, Xue Bai, Jia Zhang, Zhaoxuan Wang, Min Wang, Qian Guan, Ming Cai, Guogui Ning, Xiujuan Jia, Benoît Boachon, Sylvie Baudino, Xiang Gao
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引用次数: 0

摘要

几个世纪以来,玫瑰(蔷薇属)花迷人的香味一直吸引着人类的感官,使其成为最受欢迎和最广泛使用的花香之一。尽管在过去的十年中取得了很大进展,但造成玫瑰香味的许多生化途径仍不清楚。我们分析了不同玫瑰品种的花香成分,并选择了现代栽培品种蔷薇杂交种'双喜'作为模型系统,以揭示玫瑰优势挥发性萜烯的形成过程,这些萜烯对玫瑰香味的形成有很大贡献。研究人员对参与玫瑰萜烯生物合成的关键基因进行了功能鉴定。由香叶基/法呢基二磷酸合成酶(G/FPPS1)催化产生的胞浆香叶基二磷酸(GPP)在玫瑰香味的产生中起着关键作用,而玫瑰中的萜烯合成酶(TPSs)在大多数挥发性萜烯的形成中起着重要作用,但在香叶醇、柠檬醛和β-香茅醇的形成中却不起作用。随后,一系列酶,包括香叶醇脱氢酶(GeDH)、香叶醇还原酶(GER)、12-氧代植物二烯酸还原酶(OPR)和香茅醛还原酶(CAR)被鉴定为参与玫瑰中香叶醇通过三个连续步骤转化为 β-香茅醇的过程。有趣的是,β-香茅醇的生物合成途径似乎在其他园艺植物(如尾状花和芍药)中也得到了保留。我们的研究结果为了解玫瑰挥发性萜类化合物的生物合成提供了宝贵的信息,并为未来的育种和分子改造工作提供了重要的基因资源。
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The complexity of volatile terpene biosynthesis in roses: Particular insights into β-citronellol production.

The fascinating scent of rose (Rosa genus) flowers has captivated human senses for centuries, making them one of the most popular and widely used floral fragrances. Despite much progress over the last decade, many biochemical pathways responsible for rose scents remain unclear. We analyzed the floral scent compositions from various rose varieties and selected the modern cultivar Rosa hybrida "Double Delight" as a model system to unravel the formation of rose dominant volatile terpenes, which contribute substantially to the rose fragrance. Key genes involved in rose terpene biosynthesis were functionally characterized. Cytosolic geranyl diphosphate (GPP) generated by geranyl/farnesyl diphosphate synthase (G/FPPS1) catalysis played a pivotal role in rose scent production, and terpene synthases in roses play an important role in the formation of most volatile terpenes, but not for geraniol, citral, or β-citronellol. Subsequently, a series of enzymes, including geraniol dehydrogenase, geranial reductase, 12-oxophytodienoate reductase, and citronellal reductase, were characterized as involved in the transformation of geraniol to β-citronellol in roses through three successive steps. Interestingly, the β-citronellol biosynthesis pathway appears to be conserved in other horticultural plants like Lagerstroemia caudata and Paeonia lactiflora. Our findings provide valuable insights into the biosynthesis of rose volatile terpenoid compounds and offer essential gene resources for future breeding and molecular modification efforts.

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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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