{"title":"榄香脂对草铵膦的代谢抗性机理","authors":"","doi":"10.1016/j.pestbp.2024.106083","DOIUrl":null,"url":null,"abstract":"<div><p><em>Eleusine indica</em> is one of the most troublesome weeds in farmland worldwide, especially in Citrus Orchard of China. Glufosinate, as an efficient non-selective broad-spectrum herbicide, has been widely utilized for the control of <em>E. indica</em> in Citrus Orchard. The <em>E. indica</em> resistant population (R) was collected from a Citrus Orchard in Yichang City in Hubei province, China. Bioassay experiments showed that the R plants exhibited 3-fold resistance to glufosinate compared with the <em>E. indica</em> susceptible population (S). No known glutamine synthetase (<em>GS</em>) gene mutation associated with glufosinate resistance was found in R plants. And there was also no significant difference in GS activity between R and S plants. Those results indicated that the resistance to glufosinate in R did not involve target-site resistance. However, glutathione <em>S</em>-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) plus glufosinate gave a better control of R plants compared with glufosinate treatment alone. Moreover, both before and after glufosinate treatment, the GST activity in R plants was significantly higher than that in S plants. By RNA-seq, the expression of <em>GSTU6</em> and <em>GST4</em> up-regulated in R plants relative to S plants with or without glufosinate treatment. They were also significantly up-regulated expression in <em>E. indica</em> field resistant populations compared with S population. In summary, the study elucidated that R plants developed metabolic resistance to glufosinate involving GST. And <em>GSTU6</em> and <em>GST4</em> genes may play an important role in this glufosinate metabolic resistance. The research results provide a theoretical basis for a deeper understanding of resistance mechanism to glufosinate in <em>E. indica</em>.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolic resistance mechanism to glufosinate in Eleusine indica\",\"authors\":\"\",\"doi\":\"10.1016/j.pestbp.2024.106083\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Eleusine indica</em> is one of the most troublesome weeds in farmland worldwide, especially in Citrus Orchard of China. Glufosinate, as an efficient non-selective broad-spectrum herbicide, has been widely utilized for the control of <em>E. indica</em> in Citrus Orchard. The <em>E. indica</em> resistant population (R) was collected from a Citrus Orchard in Yichang City in Hubei province, China. Bioassay experiments showed that the R plants exhibited 3-fold resistance to glufosinate compared with the <em>E. indica</em> susceptible population (S). No known glutamine synthetase (<em>GS</em>) gene mutation associated with glufosinate resistance was found in R plants. And there was also no significant difference in GS activity between R and S plants. Those results indicated that the resistance to glufosinate in R did not involve target-site resistance. However, glutathione <em>S</em>-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) plus glufosinate gave a better control of R plants compared with glufosinate treatment alone. Moreover, both before and after glufosinate treatment, the GST activity in R plants was significantly higher than that in S plants. By RNA-seq, the expression of <em>GSTU6</em> and <em>GST4</em> up-regulated in R plants relative to S plants with or without glufosinate treatment. They were also significantly up-regulated expression in <em>E. indica</em> field resistant populations compared with S population. In summary, the study elucidated that R plants developed metabolic resistance to glufosinate involving GST. And <em>GSTU6</em> and <em>GST4</em> genes may play an important role in this glufosinate metabolic resistance. The research results provide a theoretical basis for a deeper understanding of resistance mechanism to glufosinate in <em>E. indica</em>.</p></div>\",\"PeriodicalId\":19828,\"journal\":{\"name\":\"Pesticide Biochemistry and Physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticide Biochemistry and Physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S004835752400316X\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S004835752400316X","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
榄香脂属(Eleusine indica)是全球农田中最棘手的杂草之一,尤其是在中国的柑橘园中。草铵膦作为一种高效的非选择性广谱除草剂,已被广泛应用于柑橘园的榄香酢浆草防除。从中国湖北省宜昌市的柑橘园中采集到了对茚虫威有抗性的种群(R)。生物测定实验表明,与茚虫威易感种群(S)相比,R 株对草铵膦的抗性是 S 株的 3 倍。在 R 型植株中没有发现与草铵膦抗性相关的谷氨酰胺合成酶(GS)基因突变。R 型植株和 S 型植株的 GS 活性也没有明显差异。这些结果表明,R 植物对草铵膦的抗性不涉及靶点抗性。然而,与单独使用草铵膦处理相比,谷胱甘肽 S 转移酶(GST)抑制剂 4-氯-7-硝基苯并噁二唑(NBD-Cl)加草铵膦能更好地控制 R 植物。此外,在草铵膦处理前后,R 植物的 GST 活性都明显高于 S 植物。通过RNA-seq分析,相对于施用或未施用草铵膦处理的S植株,R植株中GSTU6和GST4的表达量上调。与 S 种群相比,它们在籼稻田间抗性种群中的表达也明显上调。综上所述,该研究阐明了 R 型植株对草铵膦产生的代谢抗性涉及 GST。而 GSTU6 和 GST4 基因可能在这种草铵膦代谢抗性中发挥了重要作用。研究结果为深入了解籼稻对草铵膦的抗性机制提供了理论依据。
Metabolic resistance mechanism to glufosinate in Eleusine indica
Eleusine indica is one of the most troublesome weeds in farmland worldwide, especially in Citrus Orchard of China. Glufosinate, as an efficient non-selective broad-spectrum herbicide, has been widely utilized for the control of E. indica in Citrus Orchard. The E. indica resistant population (R) was collected from a Citrus Orchard in Yichang City in Hubei province, China. Bioassay experiments showed that the R plants exhibited 3-fold resistance to glufosinate compared with the E. indica susceptible population (S). No known glutamine synthetase (GS) gene mutation associated with glufosinate resistance was found in R plants. And there was also no significant difference in GS activity between R and S plants. Those results indicated that the resistance to glufosinate in R did not involve target-site resistance. However, glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) plus glufosinate gave a better control of R plants compared with glufosinate treatment alone. Moreover, both before and after glufosinate treatment, the GST activity in R plants was significantly higher than that in S plants. By RNA-seq, the expression of GSTU6 and GST4 up-regulated in R plants relative to S plants with or without glufosinate treatment. They were also significantly up-regulated expression in E. indica field resistant populations compared with S population. In summary, the study elucidated that R plants developed metabolic resistance to glufosinate involving GST. And GSTU6 and GST4 genes may play an important role in this glufosinate metabolic resistance. The research results provide a theoretical basis for a deeper understanding of resistance mechanism to glufosinate in E. indica.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.