紫外线诱导的地衣黑化:生理特征和转录组特征。

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-09-01 DOI:10.1111/ppl.14512
Ilya Leksin, Mikhail Shelyakin, Ilya Zakhozhiy, Olga Kozlova, Richard Beckett, Farida Minibayeva
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引用次数: 0

摘要

地衣是高纬度北方和北极栖息地的重要组成部分。虽然地衣耐受压力,但它们是对气候变化最敏感的生态系统组成部分之一,特别是极地臭氧消耗和森林砍伐导致的紫外线(UV)增加。本研究首次探讨了紫外线-B 对地衣基因表达的影响,以预测地衣耐受性所涉及的代谢途径。利用转录组分析和生物信息学分析,我们以 Lobaria pulmonaria (L.) Hoff.为模式物种,研究了紫外线-B 对地衣基因表达的影响。紫外线-B照射会导致苔藓上部皮层明显褐化,这与参与合成黑色素和异黑色素以及黑色素前体的生物合成基因簇的表达增加有关。根据转录组分析,我们认为黑色素和其他次生代谢物(如萘衍生物、萘酮类、蒽醌类和黄酮类)的生物合成是地衣为保护光合作用和呼吸作用等基本代谢过程而付出的代价。一般应激相关基因的表达谱,特别是与活性氧清除、蛋白质保护和 DNA 修复有关的基因的表达谱,清楚地表明在地衣共生中,菌丝体是对紫外线-B 反应更强、更易受影响的伙伴。我们的研究结果表明,紫外线-B 胁迫激活了地衣共生体耐受机制中错综复杂的基因网络。这些知识有助于预测气候变化和污染可能对地衣生物多样性造成的影响。
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Ultraviolet-induced melanisation in lichens: physiological traits and transcriptome profile.

Lichens are important components of high-latitude boreal and Arctic habitats. While stress tolerant, they are among the most sensitive ecosystem components to climate change, in particular, an increase in ultraviolet light (UV) arising from polar ozone depletion and deforestation. This study is the first to explore the effects of UV-B on gene expression in lichens to predict metabolic pathways involved in tolerance. Using transcriptome profiling and bioinformatic analyses, here we studied the effects of UV-B on gene expression in lichens using Lobaria pulmonaria (L.) Hoff. as a model species. UV-B exposure causes significant browning of the upper cortex of the thallus, which correlates to an increased expression of biosynthetic gene clusters involved in the synthesis of eu- and allomelanins and melanin precursors. Based on transcriptome analyses, we suggest that the biosynthesis of melanins and other secondary metabolites, such as naphthalene derivates, tropolones, anthraquinones, and xanthones, is a trade-off that lichens pay to protect essential metabolic processes such as photosynthesis and respiration. Expression profiles of general stress-associated genes, in particular, related to reactive oxygen species scavenging, protection of proteins, and DNA repair, clearly indicate that the mycobiont is the more UV-B-responsive and susceptible partner in lichen symbiosis. Our findings demonstrate that UV-B stress activates an intricate gene network involved in tolerance mechanisms of lichen symbionts. Knowledge obtained here may enable the prediction of likely effects on lichen biodiversity caused by climate change and pollution.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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