掺杂 Cu2+ 的毛刷石水泥应用相关特性的新见解。

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-09-03 DOI:10.1002/jbm.b.35479
Karla Spaeth, Qaisar Nawaz, Tatjana Schilling, Friedlinde Goetz-Neunhoeffer, Rainer Detsch, Aldo R. Boccaccini, Katrin Hurle
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引用次数: 0

摘要

在刷石水泥中掺入金属离子可对生物和物理化学特性产生许多积极影响。众所周知,Cu2+ 离子具有抗菌特性,作为微量元素还能对细胞产生不同的积极影响,而高浓度的 Cu2+ 则具有细胞毒性。骨水泥的治疗应用需要良好的生物相容性和足够的机械性能。因此,本研究的目的是研究掺有 Cu2+ 的β-磷酸三钙、一水磷酸一钙和植酸作为缓凝剂的刷状骨水泥的不同物理化学和生物方面的应用。此外,还将离子释放量与以柠檬酸作为缓凝剂的水泥进行了比较。所研究的水泥显示出良好的可注入性系数,以及足以应用的抗压强度值。此外,无论 Cu2+ 浓度或细菌菌株如何,均未发现抗菌效果。用洗脱液样品进行的细胞实验表明,随着水泥中 Cu2+ 浓度的增加,MC3T3-E1 细胞的存活率呈下降趋势。这表明这些生物反应是由硬化水泥中的 Cu2+ 释放量因溶剂介质而异造成的。此外,与使用柠檬酸作为缓凝剂的水泥相比,这些水泥显示出稳定的 Cu2+ 离子释放,但释放程度较低。总之,尽管掺入 Cu2+ 的水门汀缺乏预期的抗菌效果,哺乳动物细胞的活力也受到轻微影响,但 Cu2+ 浓度保持了水门汀的物理化学特性和抗压强度,而且与基于柠檬酸的配方相比,植酸生产的水门汀离子释放速度较慢,这一点被认为具有优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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New Insights Into Application Relevant Properties of Cu2+-Doped Brushite Cements

Doping of brushite cements with metal ions can entail many positive effects on biological and physicochemical properties. Cu2+ ions are known to exhibit antibacterial properties and can additionally have different positive effects on cells as trace elements, whereas high Cu2+ concentrations are cytotoxic. For therapeutical applications of bone cement, a combination of good biocompatibility and sufficient mechanical properties is required. Therefore, the aim of this study was to investigate different physicochemical and biological aspects, relevant for application, of a brushite cement with Cu2+-doped β-tricalcium phosphate, monocalcium phosphate monohydrate and phytic acid as setting retarder. Additionally, the ion release was compared with a cement with citric acid as setting retarder. The investigated cements showed good injectability coefficients, as well as compressive strength values sufficient for application. Furthermore, no antibacterial effects were detected irrespective of the Cu2+ concentration or the bacterial strain. The cell experiments with eluate samples showed that the viability of MC3T3-E1 cells tended to decrease with increasing Cu2+ concentration in the cement. It is suggested that these biological responses are caused by the difference in the Cu2+ release from the hardened cement depending on the solvent medium. Furthermore, the cements showed a steady release of Cu2+ ions to a lesser extent in comparison with a cement with citric acid as setting retarder, where a burst release of Cu2+ was observed. In conclusion, despite the anticipated antibacterial effect of Cu2+-doped cements was lacking and mammalian cell viability was slightly affected, Cu2+-concentrations maintained the physicochemical properties as well as the compressive strength of cements and the slow ion release from cements produced with phytic acid is considered advantageous compared to citric acid-based formulations.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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