在大鼠乳腺组织中,边缘祖细胞和成熟细胞比基底细胞更容易受到辐射诱导的 DNA 双链断裂的影响。

IF 1.9 4区 医学 Q2 BIOLOGY Journal of Radiation Research Pub Date : 2024-09-24 DOI:10.1093/jrr/rrae067
Kento Nagata, Mayumi Nishimura, Kazuhiro Daino, Yukiko Nishimura, Yuya Hattori, Ritsuko Watanabe, Daisuke Iizuka, Akinari Yokoya, Keiji Suzuki, Shizuko Kakinuma, Tatsuhiko Imaoka
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引用次数: 0

摘要

电离辐射会促进乳腺癌的发生。DNA 双链断裂(DSB)的诱导是辐照后的初始事件,有可能导致癌变,但在组织水平上,DSB 诱导和修复的动态过程并不十分清楚。在这项研究中,我们使用了雌性大鼠,它们被认为是研究辐射对乳腺影响的有用实验模型。我们重点研究了乳腺导管不同部位的基底细胞、管腔祖细胞和成熟细胞的 DSB 动力学差异。我们使用 53BP1 病灶作为 DSB 的替代标记,在辐照后 1-24 小时对免疫染色组织切片中每个乳腺上皮细胞的 53BP1 病灶进行计数,并拟合成时间的指数函数。基底细胞被鉴定为细胞角蛋白(CK)14+细胞,管腔祖细胞被鉴定为CK8 + 18低细胞,管腔成熟细胞被鉴定为CK8 + 18高细胞。辐照后1小时,管腔细胞每个核的DSB数量往往高于基底细胞。模型分析表明,构成乳腺导管前缘的终末芽(TEB)中的基底细胞的初始DSB数量明显少于两种管腔细胞,而副导管中各类型细胞的初始DSB数量没有显著差异。乳腺上皮细胞类型和位置之间的修复率没有差异。因此,管腔祖细胞和成熟细胞比TEB的基底细胞更容易受到辐射诱导的DSB的影响。
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Luminal progenitor and mature cells are more susceptible than basal cells to radiation-induced DNA double-strand breaks in rat mammary tissue.

Ionizing radiation promotes mammary carcinogenesis. Induction of DNA double-strand breaks (DSBs) is the initial event after radiation exposure, which can potentially lead to carcinogenesis, but the dynamics of DSB induction and repair are not well understood at the tissue level. In this study, we used female rats, which have been recognized as a useful experimental model for studying radiation effects on the mammary gland. We focused on differences in DSB kinetics among basal cells, luminal progenitor and mature cells in different parts of the mammary duct. 53BP1 foci were used as surrogate markers of DSBs, and 53BP1 foci in each mammary epithelial cell in immunostained tissue sections were counted 1-24 h after irradiation and fitted to an exponential function of time. Basal cells were identified as cytokeratin (CK) 14+ cells, luminal progenitor cells as CK8 + 18low cells and luminal mature cells as CK8 + 18high cells. The number of DSBs per nucleus tended to be higher in luminal cells than basal cells at 1 h post-irradiation. A model analysis indicated that basal cells in terminal end buds (TEBs), which constitute the leading edge of the mammary duct, had significantly fewer initial DSBs than the two types of luminal cells, and there was no significant difference in initial amount among the cell types in the subtending duct. The repair rate did not differ among mammary epithelial cell types or their locations. Thus, luminal progenitor and mature cells are more susceptible to radiation-induced DSBs than are basal cells in TEBs.

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来源期刊
CiteScore
3.60
自引率
5.00%
发文量
86
审稿时长
4-8 weeks
期刊介绍: The Journal of Radiation Research (JRR) is an official journal of The Japanese Radiation Research Society (JRRS), and the Japanese Society for Radiation Oncology (JASTRO). Since its launch in 1960 as the official journal of the JRRS, the journal has published scientific articles in radiation science in biology, chemistry, physics, epidemiology, and environmental sciences. JRR broadened its scope to include oncology in 2009, when JASTRO partnered with the JRRS to publish the journal. Articles considered fall into two broad categories: Oncology & Medicine - including all aspects of research with patients that impacts on the treatment of cancer using radiation. Papers which cover related radiation therapies, radiation dosimetry, and those describing the basis for treatment methods including techniques, are also welcomed. Clinical case reports are not acceptable. Radiation Research - basic science studies of radiation effects on livings in the area of physics, chemistry, biology, epidemiology and environmental sciences. Please be advised that JRR does not accept any papers of pure physics or chemistry. The journal is bimonthly, and is edited and published by the JRR Editorial Committee.
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