Muhammad Khari Secario , Thi Tuong Vi Truong , Chien-Chang Chen , Jui-Yang Lai , Shingjiang Jessie Lue
{"title":"探索抗菌效果：针对金黄色葡萄球菌的银纳米粒子绿色合成与化学合成的比较分析","authors":"Muhammad Khari Secario , Thi Tuong Vi Truong , Chien-Chang Chen , Jui-Yang Lai , Shingjiang Jessie Lue","doi":"10.1016/j.jtice.2024.105750","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Silver nanoparticles (AgNPs) are renowned for their broad-spectrum antibacterial properties. Various synthesis methods, particularly green synthesis using biogenic agents, have garnered significant attention. However, the detailed impact of green-synthesized AgNPs on the antibacterial mechanism against <em>Staphylococcus aureus</em> remain unclear, limiting the full potential of green synthesis compared to chemical methods.</p></div><div><h3>Methods</h3><p>AgNPs were synthesized via chemical (sodium citrate, NaBH<sub>4</sub>) and green synthesis (green tea leaves, cassia seed extract) methods. The synthesized AgNPs were evaluated for toxicity and antibacterial activity against <em>Staphylococcus aureus</em>.</p></div><div><h3>Significant findings</h3><p>This study revealed a strong correlation (R<sup>2</sup>>0.9) between minimal inhibitory concentration (MIC) and AgNP size for both synthesis methods, with a similar exponential trend. MIC values were 45, 40, 25, and 5 µg mL<sup>-1</sup> for citrate-, NaBH<sub>4</sub>-, green tea extract-, and cassia seed extract-assisted synthesis, respectively. Green-synthesized AgNPs showed higher antibacterial activity than chemical AgNPs at comparable sizes. Chemical AgNPs exhibited low and fluctuating scavenging activity, while green methods were more consistent. Cytotoxicity was noted in chemical AgNPs and at high concentrations of green tea extract-assisted AgNPs. Bacterial membrane disruption and ROS accumulation were also observed, contributing to the enhanced antibacterial activity of green-synthesized AgNPs.</p></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"165 ","pages":"Article 105750"},"PeriodicalIF":5.5000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring antibacterial effectiveness: A comparative analysis of green and chemical synthesis of silver nanoparticles against Staphylococcus aureus\",\"authors\":\"Muhammad Khari Secario , Thi Tuong Vi Truong , Chien-Chang Chen , Jui-Yang Lai , Shingjiang Jessie Lue\",\"doi\":\"10.1016/j.jtice.2024.105750\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Silver nanoparticles (AgNPs) are renowned for their broad-spectrum antibacterial properties. Various synthesis methods, particularly green synthesis using biogenic agents, have garnered significant attention. However, the detailed impact of green-synthesized AgNPs on the antibacterial mechanism against <em>Staphylococcus aureus</em> remain unclear, limiting the full potential of green synthesis compared to chemical methods.</p></div><div><h3>Methods</h3><p>AgNPs were synthesized via chemical (sodium citrate, NaBH<sub>4</sub>) and green synthesis (green tea leaves, cassia seed extract) methods. The synthesized AgNPs were evaluated for toxicity and antibacterial activity against <em>Staphylococcus aureus</em>.</p></div><div><h3>Significant findings</h3><p>This study revealed a strong correlation (R<sup>2</sup>>0.9) between minimal inhibitory concentration (MIC) and AgNP size for both synthesis methods, with a similar exponential trend. MIC values were 45, 40, 25, and 5 µg mL<sup>-1</sup> for citrate-, NaBH<sub>4</sub>-, green tea extract-, and cassia seed extract-assisted synthesis, respectively. Green-synthesized AgNPs showed higher antibacterial activity than chemical AgNPs at comparable sizes. Chemical AgNPs exhibited low and fluctuating scavenging activity, while green methods were more consistent. Cytotoxicity was noted in chemical AgNPs and at high concentrations of green tea extract-assisted AgNPs. Bacterial membrane disruption and ROS accumulation were also observed, contributing to the enhanced antibacterial activity of green-synthesized AgNPs.</p></div>\",\"PeriodicalId\":381,\"journal\":{\"name\":\"Journal of the Taiwan Institute of Chemical Engineers\",\"volume\":\"165 \",\"pages\":\"Article 105750\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2024-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Taiwan Institute of Chemical Engineers\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1876107024004085\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Taiwan Institute of Chemical Engineers","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1876107024004085","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
Exploring antibacterial effectiveness: A comparative analysis of green and chemical synthesis of silver nanoparticles against Staphylococcus aureus
Background
Silver nanoparticles (AgNPs) are renowned for their broad-spectrum antibacterial properties. Various synthesis methods, particularly green synthesis using biogenic agents, have garnered significant attention. However, the detailed impact of green-synthesized AgNPs on the antibacterial mechanism against Staphylococcus aureus remain unclear, limiting the full potential of green synthesis compared to chemical methods.
Methods
AgNPs were synthesized via chemical (sodium citrate, NaBH4) and green synthesis (green tea leaves, cassia seed extract) methods. The synthesized AgNPs were evaluated for toxicity and antibacterial activity against Staphylococcus aureus.
Significant findings
This study revealed a strong correlation (R2>0.9) between minimal inhibitory concentration (MIC) and AgNP size for both synthesis methods, with a similar exponential trend. MIC values were 45, 40, 25, and 5 µg mL-1 for citrate-, NaBH4-, green tea extract-, and cassia seed extract-assisted synthesis, respectively. Green-synthesized AgNPs showed higher antibacterial activity than chemical AgNPs at comparable sizes. Chemical AgNPs exhibited low and fluctuating scavenging activity, while green methods were more consistent. Cytotoxicity was noted in chemical AgNPs and at high concentrations of green tea extract-assisted AgNPs. Bacterial membrane disruption and ROS accumulation were also observed, contributing to the enhanced antibacterial activity of green-synthesized AgNPs.
期刊介绍：
Journal of the Taiwan Institute of Chemical Engineers (formerly known as Journal of the Chinese Institute of Chemical Engineers) publishes original works, from fundamental principles to practical applications, in the broad field of chemical engineering with special focus on three aspects: Chemical and Biomolecular Science and Technology, Energy and Environmental Science and Technology, and Materials Science and Technology. Authors should choose for their manuscript an appropriate aspect section and a few related classifications when submitting to the journal online.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
