产生神经源性膀胱的新型骶脊损伤大鼠模型及其功能和机制研究

IF 4.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomolecules Pub Date : 2024-09-09 DOI:10.3390/biom14091141
Kaiping Bai, Yanping Hou, Zhiyuan Zhang, Fei Yuan, Xiaoling Huang, Pengtao Liu, Xiangyu Zou, Jie Sun
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引用次数: 0

摘要

骶脊髓损伤(SSCI)会破坏膀胱神经调节并损害逼尿肌功能。目前的研究对与 SSCI 相关的神经源性下尿路功能障碍(NLUTD)的组织学和遗传学变化提供的信息有限,因此治疗方案很少。本研究旨在建立一个简单的 SSCI 动物模型,以更好地了解疾病的进展。90只8周大的Sprague-Dawley(SD)大鼠被随机分为假手术组和SSCI组。SSCI组进行了骶脊髓损伤,而假手术组则没有。在损伤后的不同时间间隔(1、2、3、4 和 6 周)进行尿动力学和组织学评估,以阐明疾病过程。尿动力学检查显示,SSCI 组的膀胱功能障碍比假组要严重,在损伤后 3-4 周左右趋于稳定。组织学检查(包括苏木精-伊红和马森三色染色)将这些功能变化与膀胱微结构改变联系起来。对假性组和 SSCI 组 6 周时的膀胱组织进行了 RNA 序列分析,以确定差异表达的基因和通路。利用聚合酶链反应(PCR)对所选基因进行了进一步分析。研究结果表明,SSCI 术后头两周出现明显的炎症反应,3-4 周后发展为膀胱纤维化。总之,这项研究提出了一种可靠、可重复、简单易行的 SSCI 模型,有助于深入了解 SSCI 后膀胱功能和形态的改变,并为未来的治疗研究奠定了基础。
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A New Rat Model of Sacral Cord Injury Producing a Neurogenic Bladder and Its Functional and Mechanistic Studies
Sacral spinal cord injury (SSCI) can disrupt bladder neuromodulation and impair detrusor function. Current studies provide limited information on the histologic and genetic changes associated with SSCI-related neurogenic lower urinary tract dysfunction (NLUTD), resulting in few treatment options. This study aimed to establish a simple animal model of SSCI to better understand the disease progression. Ninety 8-week-old Sprague-Dawley (SD) rats were randomly separated into sham operation and SSCI groups. The SSCI group underwent sacral spinal cord injury, while the sham group did not. Urodynamic and histological assessments were conducted at various intervals (1, 2, 3, 4, and 6 weeks) post-injury to elucidate the disease process. Urodynamic examinations revealed significant bladder dysfunction in the SSCI group compared to the sham group, stabilizing around 3–4 weeks post-injury. Histological examination, including hematoxylin–eosin and Masson’s trichrome staining, correlated these functional changes with bladder microstructural alterations. RNA-seq was performed on bladder tissues from the sham group and SSCI group at 6 weeks to identify differentially expressed genes and pathways. Selected genes were further analyzed using polymerase chain reaction (PCR). The findings indicated a pronounced inflammatory response in the first 2 weeks post-SSCI, progressing to bladder fibrosis at 3–4 weeks. In conclusion, this study presents a reliable, reproducible, and straightforward SSCI model, providing insights into bladder functional and morphological alterations post-SSCI and laying the groundwork for future therapeutic research.
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来源期刊
Biomolecules
Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
9.40
自引率
3.60%
发文量
1640
审稿时长
18.28 days
期刊介绍: Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications.  Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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