基于定量感应调控的厌氧颗粒污泥持久抗冲击性的新见解:一种新型基因调控机制

IF 7.4 Q1 ENGINEERING, ENVIRONMENTAL ACS ES&T engineering Pub Date : 2024-09-03 DOI:10.1021/acsestengg.4c00334
Longyi Lv, Ziyin Wei, Chendi Feng, Jiarui Chen, Weiguang Li, Jiyong Bian, Zhijun Ren, Guangming Zhang
{"title":"基于定量感应调控的厌氧颗粒污泥持久抗冲击性的新见解:一种新型基因调控机制","authors":"Longyi Lv, Ziyin Wei, Chendi Feng, Jiarui Chen, Weiguang Li, Jiyong Bian, Zhijun Ren, Guangming Zhang","doi":"10.1021/acsestengg.4c00334","DOIUrl":null,"url":null,"abstract":"Anaerobic granular sludge (AnGS) is valuable for the treatment of high concentration organic wastewater but is hampered from further development by poor stability. Quorum sensing (QS) has been shown as an effective strategy to enhance the stability of AnGS, whereas the long-term resistance and underlying mechanisms remain uncertain. This work investigates the reinforcing effect of the QS on AnGS and the internal regulation mechanisms. The chemical oxygen demand (COD) removal was maintained at preshock level (90–93%) after multiple temperature shocks in the <i>N</i>-acyl-homoserine lactones (AHLs)-induced system. AHLs-mediated QS led to an increase in gene abundance of the four hydrophobic amino acids with protein (PN) increasing by 33.1%, which optimized the construction of the protective barrier of extracellular polymeric substances (EPS). AHLs also reshaped the functional microbial community and enhanced metabolic activities, promoting both the hydrogenotrophic and methanotrophic methanogenic pathways. In addition, the abundance of <i>Geobacter</i> and <i>Methanothrix</i> was increased by 4.4% and 2.3% under the stimulation of exogenous AHLs, which enhanced the direct interspecies electron transfer (DIET) pathway. This study provides a strategy for enhancing the stability of AnGS in the face of environmental shocks and gives a comprehensive theoretical foundation for exogenous AHLs-mediated QS regulation of anaerobic biological treatment.","PeriodicalId":7008,"journal":{"name":"ACS ES&T engineering","volume":"5 1","pages":""},"PeriodicalIF":7.4000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"New Insights into the Persistent Shock Resistance of Anaerobic Granular Sludge Based on Quorum Sensing Regulation: A Novel Gene Regulatory Mechanism\",\"authors\":\"Longyi Lv, Ziyin Wei, Chendi Feng, Jiarui Chen, Weiguang Li, Jiyong Bian, Zhijun Ren, Guangming Zhang\",\"doi\":\"10.1021/acsestengg.4c00334\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Anaerobic granular sludge (AnGS) is valuable for the treatment of high concentration organic wastewater but is hampered from further development by poor stability. Quorum sensing (QS) has been shown as an effective strategy to enhance the stability of AnGS, whereas the long-term resistance and underlying mechanisms remain uncertain. This work investigates the reinforcing effect of the QS on AnGS and the internal regulation mechanisms. The chemical oxygen demand (COD) removal was maintained at preshock level (90–93%) after multiple temperature shocks in the <i>N</i>-acyl-homoserine lactones (AHLs)-induced system. AHLs-mediated QS led to an increase in gene abundance of the four hydrophobic amino acids with protein (PN) increasing by 33.1%, which optimized the construction of the protective barrier of extracellular polymeric substances (EPS). AHLs also reshaped the functional microbial community and enhanced metabolic activities, promoting both the hydrogenotrophic and methanotrophic methanogenic pathways. In addition, the abundance of <i>Geobacter</i> and <i>Methanothrix</i> was increased by 4.4% and 2.3% under the stimulation of exogenous AHLs, which enhanced the direct interspecies electron transfer (DIET) pathway. This study provides a strategy for enhancing the stability of AnGS in the face of environmental shocks and gives a comprehensive theoretical foundation for exogenous AHLs-mediated QS regulation of anaerobic biological treatment.\",\"PeriodicalId\":7008,\"journal\":{\"name\":\"ACS ES&T engineering\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":7.4000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS ES&T engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1021/acsestengg.4c00334\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS ES&T engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsestengg.4c00334","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0

摘要

厌氧颗粒污泥(AnGS)在处理高浓度有机废水方面很有价值,但由于稳定性差而无法进一步发展。定量感应(QS)已被证明是增强 AnGS 稳定性的有效策略,但其长期抗性和内在机制仍不确定。本研究探讨了 QS 对 AnGS 的强化作用及其内部调控机制。在 N-酰基高丝氨酸内酯(AHLs)诱导的系统中,经过多次温度冲击后,化学需氧量(COD)去除率保持在冲击前水平(90-93%)。AHLs 介导的 QS 导致四种疏水氨基酸的基因丰度增加,蛋白质(PN)增加了 33.1%,从而优化了细胞外聚合物质(EPS)保护屏障的构建。AHL 还重塑了功能微生物群落,增强了代谢活动,促进了亲氢型和亲甲烷型产甲烷途径。此外,在外源 AHLs 的刺激下,Geobacter 和 Methanothrix 的丰度分别增加了 4.4% 和 2.3%,从而增强了种间直接电子传递(DIET)途径。这项研究为提高AnGS在环境冲击下的稳定性提供了一种策略,并为外源AHLs介导的厌氧生物处理QS调控提供了全面的理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
New Insights into the Persistent Shock Resistance of Anaerobic Granular Sludge Based on Quorum Sensing Regulation: A Novel Gene Regulatory Mechanism
Anaerobic granular sludge (AnGS) is valuable for the treatment of high concentration organic wastewater but is hampered from further development by poor stability. Quorum sensing (QS) has been shown as an effective strategy to enhance the stability of AnGS, whereas the long-term resistance and underlying mechanisms remain uncertain. This work investigates the reinforcing effect of the QS on AnGS and the internal regulation mechanisms. The chemical oxygen demand (COD) removal was maintained at preshock level (90–93%) after multiple temperature shocks in the N-acyl-homoserine lactones (AHLs)-induced system. AHLs-mediated QS led to an increase in gene abundance of the four hydrophobic amino acids with protein (PN) increasing by 33.1%, which optimized the construction of the protective barrier of extracellular polymeric substances (EPS). AHLs also reshaped the functional microbial community and enhanced metabolic activities, promoting both the hydrogenotrophic and methanotrophic methanogenic pathways. In addition, the abundance of Geobacter and Methanothrix was increased by 4.4% and 2.3% under the stimulation of exogenous AHLs, which enhanced the direct interspecies electron transfer (DIET) pathway. This study provides a strategy for enhancing the stability of AnGS in the face of environmental shocks and gives a comprehensive theoretical foundation for exogenous AHLs-mediated QS regulation of anaerobic biological treatment.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
ACS ES&T engineering
ACS ES&T engineering ENGINEERING, ENVIRONMENTAL-
CiteScore
8.50
自引率
0.00%
发文量
0
期刊介绍: ACS ES&T Engineering publishes impactful research and review articles across all realms of environmental technology and engineering, employing a rigorous peer-review process. As a specialized journal, it aims to provide an international platform for research and innovation, inviting contributions on materials technologies, processes, data analytics, and engineering systems that can effectively manage, protect, and remediate air, water, and soil quality, as well as treat wastes and recover resources. The journal encourages research that supports informed decision-making within complex engineered systems and is grounded in mechanistic science and analytics, describing intricate environmental engineering systems. It considers papers presenting novel advancements, spanning from laboratory discovery to field-based application. However, case or demonstration studies lacking significant scientific advancements and technological innovations are not within its scope. Contributions containing experimental and/or theoretical methods, rooted in engineering principles and integrated with knowledge from other disciplines, are welcomed.
期刊最新文献
Issue Editorial Masthead Issue Publication Information Recognizing Excellence in Environmental Engineering Research: The 2023 ACS ES&T Engineering’s Best Paper Awards Review of Current and Future Indoor Air Purifying Technologies The Removal and Recovery of Non-orthophosphate from Wastewater: Current Practices and Future Directions
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1