三维打印双相磷酸钙陶瓷基底作为基质血管馏分细胞成骨分化的唯一诱导剂

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-09-13 DOI:10.1002/jbm.b.35482
Louis Brochet, Céline Thomann, Carlos Chocarro-Wrona, Ariana Abawi, Grégory Nolens, Christophe Marquette, Alexandre Dufour
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引用次数: 0

摘要

基质血管部分(SVF)是脂肪组织的一种衍生物,由脂肪间充质干细胞和内皮细胞组成,是血管化骨组织工程中一种很有前景的细胞来源。它与具有骨传导性的双相磷酸钙(BCP)陶瓷的结合可能是一种用于骨重建的护理点制剂。在此,我们评估了 SVF 在三维打印 BCP 植入物上的增殖和成骨分化能力,并与分离的脂肪间充质干细胞(AD-MSCs)进行了比较。从人体捐献者身上分离出的 AD-MSCs 和 SVF 被播种在塑料或三维打印 BCP 陶瓷上,这些陶瓷具有窦状或陀螺状宏观形貌,并在成骨因子存在或不存在的情况下进行培养。血管、造血和间充质干细胞表面标志物通过流式细胞术进行评估,而成骨活性则通过茜素红染色和碱性磷酸酶活性进行研究。在塑料上培养细胞时,成骨因子是激发成骨活性的必要条件,两种细胞群之间没有观察到显著差异。有趣的是,在没有分化因子的情况下,在 BCP 植入物上也能观察到成骨活性,而且两种细胞群之间的成骨活性水平和宏观形态没有明显差异。这项研究为将 BCP 支架与 SVF 结合使用,实现一步到位的骨再生手术提供了支持性数据。
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Three-Dimensionally Printed Biphasic Calcium Phosphate Ceramic Substrates as the Sole Inducer of Osteogenic Differentiation in Stromal Vascular Fraction Cells

The stromal vascular fraction (SVF) is a derivate of fat tissue comprising both adipose-derived mesenchymal stem cells and endothelial cells and serves as a promising cell source for engineering vascularized bone tissues. Its combination with osteoconductive biphasic calcium phosphate (BCP) ceramic may represent a point-of-care agent for bone reconstruction. Here we assessed the proliferation and osteogenic differentiation capacities of SVF on 3D printed BCP implants, in comparison with isolated adipose-derived mesenchymal stem cells (AD-MSCs). AD-MSCs and SVF isolated from human donors were seeded on plastic or 3D printed BCP ceramics with sinusoidal or gyroid macrotopography and cultured in the presence or absence of osteogenic factors. Vascular, hematopoietic and MSC surface markers were assessed by flow cytometry whereas osteogenic activity was investigated through alizarin red staining and alkaline phosphatase activity. Osteogenic factors were necessary to trigger osteogenic activity when cells were cultured on plastic, without significant difference observed between the two cell populations. Interestingly, osteogenic activity was observed on BCP implants in the absence of differentiation factors, without significant difference in level activity between the two cell populations and macrotopography. This study offers supportive data for the use of combined BCP scaffolds with SVF in a perspective of a one-step surgical procedure for bone regeneration.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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