揭示脯氨酸在草甘膦介导的毒性中的作用--耐受机制还是压力信号?

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-09-18 DOI:10.1111/ppl.14532
Pedro Nadais, Bruno Sousa, Maria Martins, Cláudia Pereira, Ana Marta Pereira, Fernanda Fidalgo, Cristiano Soares
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引用次数: 0

摘要

草甘膦(GLY)是使用最广泛的除草剂,由于其持续和累积使用,已被视为一种新出现的环境污染物,可能对农作物等非目标生物造成危害,破坏细胞的氧化还原平衡。因此,植物需要调整其抗氧化(AOX)机制,以便在 GLY 污染的环境中茁壮成长。脯氨酸过度积累是暴露于 GLY 的植物的常见反应,但其在 GLY 诱导的毒性中的作用仍不清楚。因此,本研究探讨了脯氨酸过度积累对 GLY 的响应是作为一种下游耐受机制还是一种预警胁迫信号。为此,研究人员使用拟南芥 Pro 生物合成基因(P5CS1)和分解基因(ProDH)的 T-DNA 突变株系,并对其对 GLY 的敏感性进行了筛选。幼苗接触 GLY(0.75 mg L-1)14 天后,除草剂导致所有基因型的生物量减少,同时 Pro 过度积累。Pro水平升高的突变体(prodh)表现出最大的生物量减少、脂质过氧化(LP)和过氧化氢(H2O2)水平升高,同时AOX系统的性能也受到影响。相反,Pro 水平较低的突变体 p5cs1-4 的 AOX 系统激活能力增强,不仅谷胱甘肽(GSH)和抗坏血酸(AsA)水平提高,而且抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)的活性也提高了。这些研究结果表明,Pro 在 GLY 暴露下的过度积累与对胁迫的敏感性而非耐受性有关,突出了其作为 GLY 对非目标植物毒性的早期预警信号和检测杂草抗性的潜力。
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Unravelling the role of proline in glyphosate‐mediated toxicity – tolerance mechanism or stress signal?
Glyphosate (GLY), the most widely used herbicide, has been regarded as an emergent environmental contaminant due to its constant and cumulative use, with potential harm to non‐target organisms, such as crops, disrupting cells' redox balance. Therefore, plants need to fine‐tune their antioxidant (AOX) mechanisms to thrive under GLY‐contaminated environments. Proline overaccumulation is a common response in plants exposed to GLY, yet its role in GLY‐induced toxicity remains unclear. Thus, this study explores whether Pro overaccumulation in response to GLY is perceived as a downstream tolerance mechanism or an early‐warning stress signal. To investigate this, Arabidopsis thaliana T‐DNA mutant lines for Pro biosynthetic (P5CS1) and catabolic genes (ProDH) were used and screened for their GLY susceptibility. Upon seedlings' exposure to GLY (0.75 mg L−1) for 14 days, the herbicide led to reduced biomass in all genotypes, accompanied by Pro overaccumulation. Mutants with heightened Pro levels (prodh) exhibited the greatest biomass reduction, increased lipid peroxidation (LP), and hydrogen peroxide (H2O2) levels, accompanied by a compromised performance of the AOX system. Conversely, p5cs1–4, mutants with lower Pro levels, demonstrated an enhanced AOX system activation, not only with increased levels of glutathione (GSH) and ascorbate (AsA), but also with increased activity of both ascorbate peroxidase (APX) and catalase (CAT). These findings suggest that Pro overaccumulation under GLY exposure is associated with stress sensitivity rather than tolerance, highlighting its potential as an early‐warning signal for GLY toxicity in non‐target plants and for detecting weed resistance.
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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