{"title":"用于检测碱性磷酸酶活性的分子样银簇激活的铕荧光。","authors":"Chen-Xi Zhao, Xiao-Xia Li, Yang Shu","doi":"10.1016/j.talanta.2024.126892","DOIUrl":null,"url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is abnormally expressed in some cancers and promotes the growth, metastasis, and invasion of cancer cells. The detection of ALP is of great significance for both pathological study and clinical detection. In this work, a europium (Eu)-based fluorescence detection sensor was prepared in a mild reaction condition. LaF<sub>3</sub>:Eu nanoparticles was mixed with ethylene imine polymer (PEI) and Ag<sup>+</sup> ions. PEI was used as stabilizer and reducing agent, and Ag<sup>+</sup> ions were reduced as molecular-like silver clusters (ML-Ag NCs). The fluorescence of LaF<sub>3</sub>:Eu nanoparticles was enhanced by ML-Ag NCs through energy transfer. When ascorbic acid 2-phosphate (AAP) was hydrolyzed to ascorbic acid (AA) in the presence of ALP, AA reduced Ag<sup>+</sup> ions to silver nanoparticles (Ag NPs) and quenched the fluorescence of LaF<sub>3</sub>:Eu/PEI/Ag. The activity of ALP was detected by measuring the fluorescence intensity of Eu<sup>3+</sup> at 618 nm. In the concentration range from 2.0 to 16.0 U/L, the fluorescence intensity ratio ((F<sub>0</sub>-F)/F<sub>0</sub>) had a linear relationship with the logarithm of ALP concentration. The limit of detection (LOD) was 1.3 U/L. Moreover, the ALP activity was detected successfully in cancer cells by this method. The sensing platform has application potential in the detection of ALP activity in biological systems.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fluorescence of europium activated by molecular-like silver clusters for the detection of alkaline phosphatase activity.\",\"authors\":\"Chen-Xi Zhao, Xiao-Xia Li, Yang Shu\",\"doi\":\"10.1016/j.talanta.2024.126892\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Alkaline phosphatase (ALP) is abnormally expressed in some cancers and promotes the growth, metastasis, and invasion of cancer cells. The detection of ALP is of great significance for both pathological study and clinical detection. In this work, a europium (Eu)-based fluorescence detection sensor was prepared in a mild reaction condition. LaF<sub>3</sub>:Eu nanoparticles was mixed with ethylene imine polymer (PEI) and Ag<sup>+</sup> ions. PEI was used as stabilizer and reducing agent, and Ag<sup>+</sup> ions were reduced as molecular-like silver clusters (ML-Ag NCs). The fluorescence of LaF<sub>3</sub>:Eu nanoparticles was enhanced by ML-Ag NCs through energy transfer. When ascorbic acid 2-phosphate (AAP) was hydrolyzed to ascorbic acid (AA) in the presence of ALP, AA reduced Ag<sup>+</sup> ions to silver nanoparticles (Ag NPs) and quenched the fluorescence of LaF<sub>3</sub>:Eu/PEI/Ag. The activity of ALP was detected by measuring the fluorescence intensity of Eu<sup>3+</sup> at 618 nm. In the concentration range from 2.0 to 16.0 U/L, the fluorescence intensity ratio ((F<sub>0</sub>-F)/F<sub>0</sub>) had a linear relationship with the logarithm of ALP concentration. The limit of detection (LOD) was 1.3 U/L. Moreover, the ALP activity was detected successfully in cancer cells by this method. The sensing platform has application potential in the detection of ALP activity in biological systems.</p>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.talanta.2024.126892\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/16 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2024.126892","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/16 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
摘要
碱性磷酸酶（ALP）在某些癌症中表达异常，会促进癌细胞的生长、转移和侵袭。检测 ALP 对病理研究和临床检测都具有重要意义。本研究在温和的反应条件下制备了一种基于铕（Eu）的荧光检测传感器。LaF3:Eu 纳米粒子与乙烯亚胺聚合物（PEI）和 Ag+ 离子混合。PEI 用作稳定剂和还原剂，Ag+ 离子被还原成分子样银簇（ML-Ag NCs）。通过能量转移，ML-Ag NCs 增强了 LaF3:Eu 纳米粒子的荧光。当抗坏血酸 2-磷酸（AAP）在 ALP 存在下水解为抗坏血酸（AA）时，AA 将 Ag+ 离子还原为银纳米颗粒（Ag NPs），并淬灭了 LaF3:Eu/PEI/Ag 的荧光。通过测量 618 纳米波长处 Eu3+ 的荧光强度来检测 ALP 的活性。在 2.0 至 16.0 U/L 的浓度范围内，荧光强度比（(F0-F)/F0）与 ALP 浓度的对数呈线性关系。检测限（LOD）为 1.3 U/L。此外，该方法还能成功检测出癌细胞中的 ALP 活性。该传感平台在检测生物系统中的 ALP 活性方面具有应用潜力。
Fluorescence of europium activated by molecular-like silver clusters for the detection of alkaline phosphatase activity.
Alkaline phosphatase (ALP) is abnormally expressed in some cancers and promotes the growth, metastasis, and invasion of cancer cells. The detection of ALP is of great significance for both pathological study and clinical detection. In this work, a europium (Eu)-based fluorescence detection sensor was prepared in a mild reaction condition. LaF3:Eu nanoparticles was mixed with ethylene imine polymer (PEI) and Ag+ ions. PEI was used as stabilizer and reducing agent, and Ag+ ions were reduced as molecular-like silver clusters (ML-Ag NCs). The fluorescence of LaF3:Eu nanoparticles was enhanced by ML-Ag NCs through energy transfer. When ascorbic acid 2-phosphate (AAP) was hydrolyzed to ascorbic acid (AA) in the presence of ALP, AA reduced Ag+ ions to silver nanoparticles (Ag NPs) and quenched the fluorescence of LaF3:Eu/PEI/Ag. The activity of ALP was detected by measuring the fluorescence intensity of Eu3+ at 618 nm. In the concentration range from 2.0 to 16.0 U/L, the fluorescence intensity ratio ((F0-F)/F0) had a linear relationship with the logarithm of ALP concentration. The limit of detection (LOD) was 1.3 U/L. Moreover, the ALP activity was detected successfully in cancer cells by this method. The sensing platform has application potential in the detection of ALP activity in biological systems.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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