Shahriar Keyhani, Mohammad Yousef Alikhani, Amin Doosti-Irani, Leili Shokoohizadeh
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Minimum biofilm inhibition concentration (MBIC) of <i>M. longifolia</i> essential oil was detected. The effect of essential oils on the expression level of the <i>oqx</i>A gene was detected by Real-time PCR.</p><p><strong>Results: </strong><i>M. longifolia</i> essential oil showed inhibitory activity against ciprofloxacin-resistant strains of <i>K. pneumoniae.</i> When <i>M. longifolia</i> essential oil was combined with ciprofloxacin, the MIC was reduced 2-4 times. In 28% of the strains, <i>M. longifolia</i> with ciprofloxacin showed a synergistic effect. <i>M. longifolia</i> essential oil reduces the strength of biofilm formation and alters the biofilm phenotype. A significant decrease in <i>oqx</i>A gene expression was observed in all isolates after treatment with <i>M. longifolia</i> essential oil.</p><p><strong>Conclusion: </strong>Based on the results of this study, it was observed that supplementing <i>M. longifolia</i> essential oil can help reduce ciprofloxacin resistance and inhibit biofilm formation in fluoroquinolone-resistant <i>K. pneumoniae</i> strains.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"552-559"},"PeriodicalIF":1.3000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389758/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of <i>Mentha longifolia</i> essential oil on <i>oqx</i>A efflux pump gene expression and biofilm formation in ciprofloxacin-resistant <i>Klebsiella pneumoniae</i> strains.\",\"authors\":\"Shahriar Keyhani, Mohammad Yousef Alikhani, Amin Doosti-Irani, Leili Shokoohizadeh\",\"doi\":\"10.18502/ijm.v16i4.16315\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Today, medicinal plants and their derivatives are considered to reduce the prevalence of antibiotic resistance. 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引用次数: 0
摘要
背景和目的：如今，药用植物及其衍生物被认为可以减少抗生素耐药性的流行。本研究旨在探讨长叶薄荷精油对环丙沙星耐药肺炎克雷伯菌株中 oqxA 外排泵基因表达和生物膜形成的影响：共研究了50株对环丙沙星耐药的肺炎克雷伯菌临床菌株。采用微流稀释法和分数抑制浓度法测定了龙脑香精油的最低抑菌浓度（MIC）及其与环丙沙星的协同作用。检测了 M. longifolia 精油的最小生物膜抑制浓度（MBIC）。通过实时 PCR 检测精油对 oqxA 基因表达水平的影响：结果：长叶木兰精油对耐环丙沙星的肺炎双球菌菌株具有抑制活性。当龙脑香精油与环丙沙星混合使用时，其 MIC 降低了 2-4 倍。在 28% 的菌株中，长叶木香精油与环丙沙星具有协同作用。长叶木兰精油可降低生物膜形成的强度并改变生物膜表型。用龙脑香叶精油处理后，所有分离物的 oqxA 基因表达量都明显下降：根据本研究的结果，补充长叶木兰精油有助于降低耐氟喹诺酮肺炎克氏菌菌株对环丙沙星的耐药性并抑制生物膜的形成。
Effect of Mentha longifolia essential oil on oqxA efflux pump gene expression and biofilm formation in ciprofloxacin-resistant Klebsiella pneumoniae strains.
Background and objectives: Today, medicinal plants and their derivatives are considered to reduce the prevalence of antibiotic resistance. The aim of this study was to investigate the effect of Mentha longifolia essential oil on oqxA efflux pump gene expression and biofilm formation in ciprofloxacin-resistant Klebsiella pneumoniae strains.
Materials and methods: A total of 50 clinical strains of K. pneumoniae resistant to ciprofloxacin were studied. The minimum inhibitory concentration (MIC) of M. longifolia essential oil and its synergistic effect with ciprofloxacin were determined using the microbroth dilution method and the fractional inhibitory concentration (FIC) method. Minimum biofilm inhibition concentration (MBIC) of M. longifolia essential oil was detected. The effect of essential oils on the expression level of the oqxA gene was detected by Real-time PCR.
Results: M. longifolia essential oil showed inhibitory activity against ciprofloxacin-resistant strains of K. pneumoniae. When M. longifolia essential oil was combined with ciprofloxacin, the MIC was reduced 2-4 times. In 28% of the strains, M. longifolia with ciprofloxacin showed a synergistic effect. M. longifolia essential oil reduces the strength of biofilm formation and alters the biofilm phenotype. A significant decrease in oqxA gene expression was observed in all isolates after treatment with M. longifolia essential oil.
Conclusion: Based on the results of this study, it was observed that supplementing M. longifolia essential oil can help reduce ciprofloxacin resistance and inhibit biofilm formation in fluoroquinolone-resistant K. pneumoniae strains.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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