用于皮肤伤口愈合的电纺壳聚糖、纳米壳聚糖和透明质酸膜的生产与表征

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-09-26 DOI:10.1002/jbm.b.35485
Edimar Dal Ponte, Alexia de Almeida Ignatowicz, Gabriel Raio Volpato, João Vitor Taffarel, Priscila Ayumi Takahashi, Rafael Messias Luiz, Felipe Eduardo Bueno Silva, Gabriel Nardi Fraga, Douglas Cardoso Dragunski, Ana Carla Zarpelon-Schutz, Helton José Alves, Juliana Bernardi-Wenzel
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引用次数: 0

摘要

如今,由生物材料制成的新型伤口敷料的开发越来越多,这种敷料具有更好的成本效益比,并能加速伤口愈合。各种生物聚合物都可以通过电纺形成用于伤口愈合的功能膜。因此,本研究利用电纺丝技术制备了含或不含透明质酸的壳聚糖和纳米壳聚糖膜,并对其在大鼠皮肤伤口愈合中的特性和效果进行了评估。使用聚环氧乙烷(PEO)制备了浓度为 1%-4%的壳聚糖和纳米壳聚糖溶液(含或不含透明质酸),并对其进行了电纺丝处理,从而获得了具有扫描电子显微镜(SEM)、机械测试和抗菌活性特征的膜。通过监测大鼠的病变面积、伤口收缩、组织学分析和诱导促炎细胞因子(IL-1 α 和 TNF-α)的产生,评估了膜的愈合效果。纳米壳聚糖膜和含有透明质酸的纳米壳聚糖膜的纤维直径更大、更均匀、抗性更强、弹性更好、热稳定性更高,而且与伤口床的粘附性和渗透能力也更好。尽管它们在体外不具有抗菌活性,但有助于测试动物体内促炎性白细胞介素的产生,提供物理保护,在评估的第七天之前更明显地缩小了伤口面积,加速了愈合过程,特别是在透明质酸功能化的情况下。这些结果表明,这种薄膜在加速人类慢性伤口的愈合过程方面大有可为。
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Production and Characterization of Electrospun Chitosan, Nanochitosan and Hyaluronic Acid Membranes for Skin Wound Healing

The development of new wound dressings made from biomaterials, which offer a better cost–benefit ratio and accelerate the healing process, is increasing nowadays. Various biopolymers can be electrospun to form functional membranes for wound healing. Therefore, in this study, chitosan and nanochitosan membranes with or without hyaluronic acid were prepared using the electrospinning technique, characterized and evaluated in the healing of skin wounds in rats. Chitosan and nanochitosan solutions, with or without hyaluronic acid, were prepared at concentrations of 1%–4% using PEO (polyethylene oxide) and subjected to the electrospinning process to obtain membranes characterized by scanning electron microscopy (SEM), mechanical tests, and antimicrobial activity. The healing effect of the membranes was evaluated by monitoring the area of the lesions, contraction of the wounds, histologic analysis, and induction of pro-inflammatory cytokine (IL-1 α and TNF-α) production in rats. The nanochitosan and nanochitosan membranes with hyaluronic acid achieved greater fiber diameter and uniformity, resistance, elasticity, and thermal stability, in addition to good adhesion to the wound bed and permeation capacity. Despite not presenting antimicrobial activity in vitro, they contributed to the production of pro-inflammatory interleukins in the animals tested, provided physical protection, reduced the wound area more markedly until the seventh day of the evaluation, with an acceleration of the healing process and especially when functionalized with hyaluronic acid. These results indicate that the membranes may be promising for accelerating the healing process of chronic wounds in humans.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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