姜黄素通过抑制炎症反应增强高海拔急性肺损伤大鼠的肺屏障功能

IF 5.8 2区 医学 Q1 Medicine Respiratory Research Pub Date : 2024-09-28 DOI:10.1186/s12931-024-02975-z
Xinyue Yang, Jiajia Li, Yan Ma, Xiang Dong, Jinquan Qu, Feixing Liang, Jiangwei Liu
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引用次数: 0

摘要

背景:暴露在高海拔地区的低压缺氧环境中会导致肺损伤。在本研究中,我们旨在确定姜黄素(Cur)是否能改善肺屏障功能并保护肺免受高海拔相关急性肺损伤:方法:将 200 只健康大鼠随机分为标准对照组、高海拔对照组(HC)、沙利度苷(40 毫克/千克,阳性对照)和姜黄素(200 毫克/千克)组。每组又分为五个亚组。对基本生命体征、肺损伤组织病理学、血常规参数、血浆乳酸水平和动脉血气指标进行评估。支气管肺泡灌洗液(BALF)中的蛋白质和炎症因子(肿瘤坏死因子α(TNF-α)、白细胞介素[IL]-1β、IL-6和IL-10)浓度分别用双喹啉酸法和酶联免疫吸附法测定。免疫印迹法分析了炎症相关蛋白和肺屏障功能相关蛋白:结果:与 HC 组相比,Cur 改善了血红蛋白和血细胞比容等血常规指标,降低了 BALF 蛋白含量和 TNF-α、IL-1β、IL-6 水平。它提高了 IL-10 水平,减轻了肺毛细血管充血、肺泡出血和肺间质水肿程度。它提高了氧分压、血氧饱和度、碳酸氢根、碱过量水平以及闭塞带 1、闭塞素、Claudin-4 的表达,降低了二氧化碳分压、血浆乳酸和磷酸核因子 kappa 的表达:结论:在高海拔环境中暴露 48 小时会导致大鼠肺部严重损伤。Cur能改善肺屏障功能,减轻高海拔地区大鼠的急性肺损伤。
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Curcumin-mediated enhancement of lung barrier function in rats with high-altitude-associated acute lung injury via inhibition of inflammatory response.

Background: Exposure to a hypobaric hypoxic environment at high altitudes can lead to lung injury. In this study, we aimed to determine whether curcumin (Cur) could improve lung barrier function and protect against high-altitude-associated acute lung injury.

Methods: Two hundred healthy rats were randomly divided into standard control, high-altitude control (HC), salidroside (40 mg/kg, positive control), and Cur (200 mg/kg) groups. Each group was further divided into five subgroups. Basic vital signs, lung injury histopathology, routine blood parameters, plasma lactate level, and arterial blood gas indicators were evaluated. Protein and inflammatory factor (tumor necrosis factor α (TNF-α), interleukin [IL]-1β, IL-6, and IL-10) concentrations in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method and enzyme-linked immunosorbent assay, respectively. Inflammation-related and lung barrier function-related proteins were analyzed using immunoblotting.

Results: Cur improved blood routine indicators such as hemoglobin and hematocrit and reduced the BALF protein content and TNF-α, IL-1β, and IL-6 levels compared with those in the HC group. It increased IL-10 levels and reduced pulmonary capillary congestion, alveolar hemorrhage, and the degree of pulmonary interstitial edema. It increased oxygen partial pressure, oxygen saturation, carbonic acid hydrogen radical, and base excess levels, and the expression of zonula occludens 1, occludin, claudin-4, and reduced carbon dioxide partial pressure, plasma lactic acid, and the expression of phospho-nuclear factor kappa.

Conclusions: Exposure to a high-altitude environment for 48 h resulted in severe lung injury in rats. Cur improved lung barrier function and alleviated acute lung injury in rats at high altitudes.

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来源期刊
Respiratory Research
Respiratory Research RESPIRATORY SYSTEM-
CiteScore
9.70
自引率
1.70%
发文量
314
审稿时长
4-8 weeks
期刊介绍: Respiratory Research publishes high-quality clinical and basic research, review and commentary articles on all aspects of respiratory medicine and related diseases. As the leading fully open access journal in the field, Respiratory Research provides an essential resource for pulmonologists, allergists, immunologists and other physicians, researchers, healthcare workers and medical students with worldwide dissemination of articles resulting in high visibility and generating international discussion. Topics of specific interest include asthma, chronic obstructive pulmonary disease, cystic fibrosis, genetics, infectious diseases, interstitial lung diseases, lung development, lung tumors, occupational and environmental factors, pulmonary circulation, pulmonary pharmacology and therapeutics, respiratory immunology, respiratory physiology, and sleep-related respiratory problems.
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