Rab4b可促进PK-15细胞在寄生璃藻毒素诱导下的细胞毒性。

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY Toxins Pub Date : 2024-09-19 DOI:10.3390/toxins16090407

Yiwen Zhang, Zhen Yang, Ke Dai, Bangdi Hu, Shiyu Xu, Yu Wang, Li Lei, Senyan Du, Qin Zhao, Xiaobo Huang, Rui Wu, Qigui Yan, Yiping Wang, Sanjie Cao, Yiping Wen

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引用次数: 0

摘要

寄生蛆虫细胞致死膨胀毒素（GpCDT）可诱导细胞周期停滞和细胞凋亡。我们实验室之前的研究表明，GTPase 4b （Rab4b）是与 GpCDT 诱导的细胞毒性有关的关键宿主蛋白。本研究调查了 Rab4b 在这一过程中的可能参与。我们的研究利用 CRISPR/Cas9 技术创建了 Rab4b 基因敲除细胞系。结果显示，该细胞系对GpCDT诱导的细胞毒性具有更强的抵抗力。相反，强迫 Rab4b 过表达会增加 GpCDT 诱导的细胞毒性。进一步的免疫沉淀研究表明，GpCDT 可能与 Rab4b 结合。在 PK-15 细胞中，GpCDT 被转运到早期内体和晚期内体，而敲除 Rab4b 后，GpCDT 无法通过囊泡转运到早期内体。在PK-15细胞中，Rab4b似乎对GpCDT诱导的细胞毒性至关重要。

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Rab4b Promotes Cytolethal Distending Toxin from Glaesserella parasuis-Induced Cytotoxicity in PK-15 Cells.

Glaesserella parasuis cytolethal distending toxin (GpCDT) can induce cell cycle arrest and apoptosis. Our laboratory's previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in GpCDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study used CRISPR/Cas9 technology to create a Rab4b-knockout cell line. The results showed greater resistance to GpCDT-induced cell cytotoxicity. In contrast, forced Rab4b overexpression increased GpCDT-induced cytotoxicity. Further immunoprecipitation study reveals that GpCDT may bind with Rab4b. In PK-15 cells, GpCDT is transported to the early endosomes and late endosomes, while after knocking out Rab4b, GpCDT cannot be transported to the early endosome via vesicles. Rab4b appears essential for GpCDT-induced cytotoxicity in PK-15 cells.

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来源期刊

Toxins TOXICOLOGY-

CiteScore

7.50

自引率

16.70%

发文量

765

审稿时长

16.24 days

期刊介绍： Toxins (ISSN 2072-6651) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to toxins and toxinology. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.