开发和评估用于检测斯里兰卡 COVID-19 患者 SARS-CoV-2 特异性抗体的内部 ELISAs。

IF 2.8 Q3 MICROBIOLOGY International Journal of Microbiology Pub Date : 2024-09-19 eCollection Date: 2024-01-01 DOI:10.1155/2024/1331067
Sisira L Pathirana, Bhagya Deepachandi, Peshala Gunasekara, Narmada Fernando, Inoka C Perera, Dakshika Gangani, James Thambyarajah, Dhanushka Dasanayake, Rajiva de Silva, Sunil Premawansa, Andreas Nitsche, Shiroma M Handunnetti
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引用次数: 0

摘要

COVID-19 血清学检测是分子诊断的补充,可作为血清监测和疫苗效率评估的重要工具。本研究旨在开发和评估用于 SARS-CoV-2 血清回顾性监测的内部 ELISA 的诊断性能。研究评估了北科伦坡教学医院 PCR 阳性 SARS-CoV-2 患者(50 人)血清中的总 IgG 和 IgM 水平,并将其与作为对照的流行前健康人血清(50 人)进行了比较。患者样本的采集是在国内广泛开展疫苗接种计划之前开始的。在确诊病例中,针对尖峰蛋白受体结合域或核壳蛋白的 IgG 或 IgM 抗 SARS-CoV-2 抗体的血清阳性率为 94.0%(n = 47/50),而对照组无血清阳性反应。相反,用商业 ELISA 试剂盒检测 IgG 或 IgM,血清阳性率仅为 48.0%(n = 24/50)。所有用市售试剂盒检测出血清阳性的样本在用内部 IgM 或 IgG ELISA 检测时血清仍呈阳性。Ab水平与发病后的采样日之间存在明显的相关性(p ≤ 0.001)。在发病后第一周、第二周和第三周或更长时间内,内部 IgM 或 IgG 酶联免疫吸附试验的总体灵敏度分别为 66.7%、96.9% 和 100.0%。大多数患者(>80.0%)血清呈阳性,与年龄(60 岁)、性别(男性与女性)或临床严重程度(轻度与中度/重度)无关。这些数据表明,开发的内部 ELISA 可用于评估自然感染或疫苗接种引起的抗 SARS-CoV-2 抗体水平。
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Development and Evaluation of In-House ELISAs for the Detection of SARS-CoV-2-Specific Antibodies in COVID-19 Patients in Sri Lanka.

COVID-19 serological tests complement the molecular diagnostics and can be used as important tool for serosurveillance and vaccine efficiency evaluation. The aim of this study was to develop and evaluate the diagnostic performance of an in-house ELISA for retrospective serosurveillance of SARS-CoV-2. Total IgG and IgM levels in sera of PCR positive SARS-CoV-2 patients (n = 50) from North Colombo Teaching Hospital were evaluated and compared with sera (n = 50) collected from prepandemic healthy individuals as controls. Patient sample collection was initiated before vaccination programme was widely started within the country. Seropositivity of 94.0% (n = 47/50) was observed for either IgG or IgM anti-SARS-CoV-2 antibodies against receptor binding domain of spike protein or nucleocapsid protein in confirmed cases while none of controls were seropositive. In contrast, the seropositivity of only 48.0% (n = 24/50) was demonstrated with commercial ELISA kits for detection of IgG or IgM. All samples detected seropositive by commercially available kits remained seropositive with either in-house IgM or IgG ELISA. Significant correlations (p ≤ 0.001) were observed between Ab levels and day of sampling from the onset of illness. The overall sensitivity values of the in-house assays were 66.7%, 96.9%, and 100.0% for the first, second, and third week or longer after onset of symptoms for either in-house IgM or IgG ELISAs. Majority of the patients (>80.0%) were seropositive, regardless of age (<60 vs. >60 years), gender (male vs. female), or clinical severity (mild vs. moderate/severe). These data suggest that the developed in-house ELISAs can be applied to assess anti-SARS-CoV-2 antibody levels induced by either natural infections or vaccination.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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