可持续掺杂碳点作为抗氧化剂和治疗用纳米载体。

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-09-25 DOI:10.1007/s10895-024-03940-1
Afzal Hussain, Mohamed Fahad Alajmi, S Ganguly
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引用次数: 0

摘要

除了荧光传感功能外,掺杂杂原子的碳点作为药用化合物的纳米载体和抗氧化剂也大有可为。我们介绍了一种利用壳聚糖和柠檬提取物一步水热偶联合成制备碳点(CLCDs)的方法。合成的 CLCDs 具有显著的胶体稳定性、抗氧化性、细胞相容性和药物分子的纳米载体。利用傅立叶变换红外光谱(FTIR)、X射线光电子能谱(XPS)、荧光光谱、电子顺磁共振(EPR)光谱和透射电子显微镜(TEM)等先进技术对纳米颗粒进行了分析,以确定其表面的精确成分。为了评估 CLCD 的药物传输特性,用抗癌药物化合物对其表面进行了进一步修饰。针对生理模拟流体和不同 pH 值环境下的药物释放行为进行了研究,结果表明在酸性条件下药物释放的延迟反应更好。在将结果拟合到 Higuchi、Weibull 和 Korsmeyer-Peppas 模型后,确认了合理的机理途径。Korsmeyer-Peppas 模型的拟合优度超过 95%,其释放机制得到了反常迁移的支持。此外,CLCDs 的自由基清除活性在低浓度(1 毫克/毫升)下也得到了证实,可以推断其对大多数测试剂(DPPH、ABTS 和羟基自由基)的功效大于 85%。因此,所制备的 CLCDs 可作为合适的纳米载体,用于有效载荷的传输,具有突出的抗氧化活性和对活细胞株的低毒性。
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Sustainable Doped Carbon Dots as Antioxidant and Nanocarrier for Therapeutic Cargos.

Aside from their fluorescence sensing capabilities, carbon dots doped with heteroatoms show tremendous promise as nanocarriers for medicinal compounds and as antioxidants. We present a method for producing carbon dots from chitosan and lemon extract (CLCDs) using a one-step hydrothermal coupling synthesis. The as-synthesized CLCDs exhibited remarkable colloidal stability, antioxidant behavior, cytocompatibility, and nanocarrier for drug molecules. The nanoparticles was analyzed using advanced techniques such as Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy, electron paramagnetic resonance (EPR) spectroscopy, and transmission electron microscopy (TEM) to determine the precise composition of their surface. In order to evaluate the drug transport properties of CLCDs, their surfaces were further modified with anticancer drug compounds. The drug release behavior was studied against physiologically simulated fluids and at different pH environments showing better delayed response in acidic condition. The plausible mechanistic pathways have been confirmed after fitting the results into Higuchi, Weibull and Korsmeyer-Peppas models. The goodness of fit was more than 95% for the Korsmeyer-Peppas model, with the release mechanism supported by anomalous transport. Moreover, the radical scavenging activity of CLCDs was also confirmed at low levels (1 mg/mL) which could be inferred > 85% efficacy against mostly employed testing agents (DPPH, ABTS, and hydroxyl radicals). Thus, the prepared CLCDs could be used as suitable nanovector in payload delivery with prominent antioxidant activity and low toxicity against living cell lines.

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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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