编辑 MeSWEET10a 启动子可使木薯栽培品种 SC8 获得细菌性枯萎病抗性。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-10-01 DOI:10.1111/mpp.70010
Yajie Wang, Mengting Geng, Ranran Pan, Tong Zhang, Xiaohua Lu, Xinghou Zhen, Yannian Che, Ruimei Li, Jiao Liu, Yinhua Chen, Jianchun Guo, Yuan Yao
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引用次数: 0

摘要

木薯淀粉是一种广泛用于工业生产的原材料,也是人们的食物来源。然而,由木薯黄单胞菌(Xanthomonas axonopodis pv. manihotis,Xam)引起的木薯细菌性疫病(CBB)会造成严重的产量损失,是全球木薯种植区最具破坏性的细菌性病害。Xam11 是来自中国的高致病性亚种,感染中国本地的木薯品种华南 8 号(SC8)后症状明显。该研究表明,Xam11菌株的转录激活子样效应子TALE20Xam11通过与木薯华南8号启动子MeSWEET10a的EBETALE20区结合,调控感病基因MeSWEET10a的表达。CRISPR/Cas9 产生的 EBETALE20 区域突变导致 MeSWEET10a 在 Xam11 感染后的表达显著减少,与野生型相比,这与疾病症状减轻、病变面积缩小和细菌增殖减少有关。重要的是,编辑后的植株在温室条件下仍能保持正常的生长、发育和产量特性。这些结果为培育抗细菌性枯萎病的木薯栽培品种 SC8 奠定了研究基础。
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Editing of the MeSWEET10a promoter yields bacterial blight resistance in cassava cultivar SC8.

Cassava starch is a widely used raw material for industrial production and food source for people. However, cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) results in severe yield losses and is the most destructive bacterial disease in all worldwide cassava-growing regions. Xam11 is a highly pathogenic subspecies from China that infects the Chinese local cassava South China No. 8 (SC8) cultivar with marked symptoms. This study showed that the transcription activator-like effector TALE20Xam11 of Xam11 strain regulates the expression of disease-susceptibility gene MeSWEET10a by binding to the EBETALE20 region of the MeSWEET10a promoter in cassava cultivar SC8. CRISPR/Cas9-generated mutations of the EBETALE20 region resulted in a significant reduction in MeSWEET10a expression after infection by Xam11, correlating with reduced disease symptoms, smaller lesion sizes and decreased bacterial proliferation compared with the wild type. Importantly, the edited plants maintained normal growth, development and yield characteristics under greenhouse conditions. The results lay a research foundation for breeding resistant cassava cultivar SC8 to bacterial blight.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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