使用滴注法诱导小鼠厌恶异氟醚。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Laboratory Animals Pub Date : 2024-10-04 DOI:10.1177/00236772241262119
Maya J Bodnar, I Joanna Makowska, Courtney T Boyd, Catherine A Schuppli, Daniel M Weary
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引用次数: 0

摘要

许多指南都认为二氧化碳安乐死前的异氟烷麻醉是一种改进。没有蒸发器的机构可以使用 "滴注 "法,即将液态麻醉剂引入诱导室。了解异氟醚的最小厌恶浓度至关重要。之前的研究表明,使用滴注法给小鼠注射浓度为 5%的异氟醚会使其产生厌恶感。其他工作表明,较低浓度(1.7% 至 3.7%)的异氟烷也可用于滴注法麻醉小鼠,但对这些浓度的厌恶程度尚未进行测试。我们使用条件性场所厌恶(CPA)范式评估了小鼠对这些较低浓度异氟醚滴注法的厌恶程度。雌性 C57BL/6J (OT-1) 小鼠(n = 28)被随机分配到三种异氟醚浓度中的一种:1.7%、2.7% 和 3.7%。小鼠适应光-暗装置。在进行暗室(+异氟醚)和光室调节之前和之后,小鼠接受了初始和最终偏好评估;初始和最终偏好测试之间小鼠在暗室内停留时间的变化被用来计算 CPA 分数。厌恶程度随着异氟烷浓度的增加而增加:异氟烷浓度从 1.7% 到 2.7% 再到 3.7%,CPA 评分的平均值(±SE)从 19.6 ± 20.1 秒下降到 -25.6 ± 23.2 秒,再下降到 -116.9 ± 30.6 秒(F1,54 = 15.4,p.
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Mouse aversion to induction with isoflurane using the drop method.

Isoflurane anesthesia prior to carbon dioxide euthanasia is recognized as a refinement by many guidelines. Facilities lacking access to a vaporizer can use the "drop" method, whereby liquid anesthetic is introduced into an induction chamber. Knowing the least aversive concentration of isoflurane is critical. Previous work has demonstrated that isoflurane administered with the drop method at a concentration of 5% is aversive to mice. Other work has shown that lower concentrations (1.7% to 3.7%) of isoflurane can be used to anesthetize mice with the drop method, but aversion to these concentrations has not been tested. We assessed aversion to these lower isoflurane concentrations administered with the drop method, using a conditioned place aversion (CPA) paradigm. Female C57BL/6J (OT-1) mice (n = 28) were randomly allocated to one of three isoflurane concentrations: 1.7%, 2.7%, and 3.7%. Mice were acclimated to a light-dark apparatus. Prior to and following dark (+ isoflurane) and light chamber conditioning sessions, mice underwent an initial and final preference assessment; the change in the duration spent within the dark chamber between the initial and final preference tests was used to calculate a CPA score. Aversion increased with increasing isoflurane concentration: from 1.7% to 2.7% to 3.7% isoflurane, mean ± SE CPA score decreased from 19.6 ± 20.1 s to -25.6 ± 23.2 s, to -116.9 ± 30.6 s (F1,54 = 15.4, p < 0.001). Our results suggest that, when using the drop method to administer isoflurane, concentrations between 1.7% and 2.7% can be used to minimize female mouse aversion to induction.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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