用于唇腭裂修复的三维冻干富血小板纤维蛋白支架的物理化学和生物相容性表征。

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241289208
Nurul Aida Ngah, Jithendra Ratnayake, George J Dias, Darryl C Tong, Siti Noor Fazliah Mohd Noor, Paul R Cooper, Haizal Mohd Hussaini
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引用次数: 0

摘要

颅面骨骼缺陷是由各种疾病造成的,如外伤、先天畸形和感染。唇腭裂是人类最常见的先天性颅面出生缺陷。生长因子(GFs)是细胞分泌的可溶性蛋白质,可调节各种细胞过程和组织再生。目前,开发用于向损伤部位输送生长因子的三维支架已成为颅颌面骨再生的一个重要方面。本研究旨在利用冻干血小板富集纤维蛋白(LyPRF)生物复合材料支架开发一种新型三维骨替代物,有望应用于中枢神经瘫痪修复。研究人员使用胶原蛋白(C)、生物玻璃(BG)和 LyPRF 制作了一种生物复合材料(C-BG-LyPRF)支架。对支架的物理、化学和生物相容性进行了评估。C-BG-LyPRF 支架的平均孔径为 146 微米,孔隙率为 87.26%。傅立叶变换红外光谱验证了酰胺Ⅰ、Ⅱ和Ⅲ官能团的存在。经 EDX 分析测定,C-BG-LyPRF 支架的无机相由钠、钙、硅和磷组成。此外,在活/死和增殖试验中,C-BG-LyPRF 支架与 MC3T3-E1 细胞均具有生物相容性。数据表明,所开发的 C-BG-LyPRF 支架具有仿生和生物相容性,是一种很有前途的颅面再生生物材料。
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Physicochemical and biocompatibility characterisation of a 3D lyophilised platelet-rich fibrin scaffold for cleft lip and palate repair.

Craniofacial bone defects result from various disorders such as trauma, congenital malformations and infections. Cleft lip and palate are the most prevalent congenital craniofacial birth defect in humans. Growth factors (GFs) are soluble proteins secreted by cells that regulate various cellular processes and tissue regeneration. At present, developing three-dimensional scaffolds for delivering GFs to the site of injury has become an important aspect in craniofacial bone regeneration. This study aims to develop a novel 3D bone substitute using lyophilized-platelet-rich fibrin (LyPRF) biocomposite scaffolds for potential application for CLP repair. Collagen (C), bioglass (BG), and LyPRF were used to fabricate a biocomposite (C-BG-LyPRF) scaffold. The physical, chemical, and biocompatibility properties of the scaffold were evaluated. The C-BG-LyPRF scaffold demonstrated a mean pore diameter of 146 µm within a porosity of 87.26%. The FTIR spectra verified the presence of am-ide I, II, and III functional groups. The inorganic phase of the C-BG-LyPRF scaffold was composed of sodium, calcium, silicon, and phosphorus, as determined by EDX analysis. Furthermore, C-BG-LyPRF scaffold was biocompatible with MC3T3-E1 cells in both the Live/Dead and prolif-eration assays. Data demonstrate the developed C-BG-LyPRF scaffold exhibits biomimetic and biocompatibility properties, establishing it as a promising biomaterial for craniofacial regeneration.

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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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