Tomotsumi Fujisawa, Nozomi Tanaka, Jun Tamogami, Masashi Unno
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引用次数: 0
摘要
感光性视网膜红蛋白 II（SRII）是一种原型光传感器，能与视网膜希夫碱发色团结合。光吸收后，SRII 转变为信号状态，已知有两种长效光中间体在其中起作用。一种是含有去质子化 13 顺式发色团的 M 中间体，另一种是据认为含有质子化全反式发色团的 O 中间体。O 中间体中的发色团也被认为具有关于席夫碱分子的非典型 15-顺式（C═N 顺式）构型。在这篇通讯中，我们利用拉曼光谱研究了 Natronomonas pharaonis 的 SRII（NpSRII），发现 O 中间体中的视网膜发色团构型是 13-顺式、15-反式（C═N 反式），这与传统观念相反。这一结果指出了 SRII 长寿命信号状态背后的发色团结构变化的修正。
Retinal Chromophore Configuration in the O Intermediate of Sensory Rhodopsin II from Natronomonas pharaonis.
Sensory rhodopsin II (SRII) is a prototype photosensor that binds the retinal Schiff base chromophore. Upon photoabsorption, SRII is transformed into the signaling state, where two long-lived photointermediates are known to contribute. One is the M intermediate containing the deprotonated 13-cis chromophore, and the other is the O intermediate that is believed to have the protonated all-trans chromophore. The chromophore in the O intermediate is also thought to have the atypical 15-syn (C═N cis) configuration about the Schiff base moiety. In this communication, we study SRII from Natronomonas pharaonis (NpSRII) using Raman spectroscopy and find that the retinal chromophore configuration in the O intermediate is the 13-cis, 15-anti (C═N trans), contrary to the conventional notion. This result points out the revision of the chromophore structural changes underlying the long-lived signaling state of SRII.
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