外源性褪黑素通过减轻氧化损伤增强芒果(Mangifera indica L.)的水分胁迫耐受性

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-09-01 DOI:10.1111/ppl.14566
Ajaya Kumar Trivedi, Sushil Kumar Shukla, Ghanshyam Pandey, Achal Singh
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引用次数: 0

摘要

在亚热带地区,4 月至 6 月是芒果树的临时水分胁迫期,会导致巨大的经济损失。虽然根系深层有水,但土壤上层的须根表面却很干燥,树木的蒸腾率很高。水分胁迫会导致氧化状态加剧,不利于果实的生长和发育。寻找水分胁迫管理的替代品对于芒果的可持续生产非常重要。为了控制芒果的湿度胁迫,我们测试了叶面喷施 20、50、100 和 150 μM 褪黑激素是否有助于维持细胞的低氧化状态。在果实发育的三个物候期(大理石期、卵期和成熟果期)对 16 年树龄的果树施用褪黑激素,并对每种处理的相同植株进行了为期三年的跟踪观察。施用褪黑素确实提高了芒果的产量。与灌溉果树相比,水分胁迫使产量降低了 55.94%,但褪黑激素处理仅降低了 7.5%。此外,在灌溉和褪黑激素处理条件下收获的 "A "级果实比非灌溉和未处理条件下多。事实上,经褪黑激素处理的水分胁迫果树叶片总叶绿素含量(12.58 mg.g-1 鲜重)远高于未处理果树(6.77 mg.g-1),与灌溉果树(12.50 mg.g-1)相近。经褪黑素处理的植物叶绿素含量的增加与剂量有关。同样,灌溉和褪黑激素处理过的树木叶片中过氧化氢酶、过氧化物酶、超氧化物酶歧化酶的活性低于非灌溉条件下,水分胁迫下褪黑激素处理过的树木(0.77 nmol H2O2.mg-1 蛋白质)和灌溉树木(0.65)的超氧化物自由径向形成低于水分胁迫下的非处理树木(4.27)。在灌溉、褪黑激素处理和非灌溉条件下,抗氧化剂(总、还原和氧化谷胱甘肽和抗坏血酸)含量和抗氧化酶活性(即谷胱甘肽还原酶和抗坏血酸过氧化物酶)均存在显著差异。总之,在果实的不同发育阶段三次施用 150 μM 的外源褪黑激素可能是管理芒果树瞬时水分胁迫的一种可持续且有用的方法,这要归功于它对抗氧化系统的积极作用。
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Exogenous Melatonin Enhances Moisture Stress Tolerance in Mango (Mangifera indica L.) through Alleviating Oxidative Damages.

In subtropical regions, April to June represents a temporary moisture stress for mango trees, leading to huge economic loss. Although water is available in the deep root zone, the upper soil surface, which has fibrous roots, is dry, and the tree transpiration rate is high. Moisture stress causes an increased oxidation state, which is detrimental to fruit growth and development. Finding substitutes for moisture stress management is important for sustainable mango production. To manage this moisture stress in mango, we tested if foliar application of 20, 50, 100 and 150 μM melatonin helped to maintain a reduced oxidation state in the cells. Applications were made at three phenological stages of fruit development (marble, egg and mature fruit stages) in 16-year-old trees and the same plants for each treatment were followed over three years. Melatonin application indeed improved the fruit yield of mango. Moisture stress decreased yield by 55.94% compared to irrigated trees but only by 7.5% in melatonin treatment. Also, more 'A' grade fruits were harvested in irrigated and melatonin-treated conditions than in non-irrigated and non-treated conditions. Indeed, the total chlorophyll content in the leaves of moisture-stressed melatonin-treated trees (12.58 mg.g-1 fresh weight) was well above non-treated trees (6.77 mg.g-1) and similar to irrigated trees (12.50 mg.g-1). A dose-dependent increase in the chlorophyll content of melatonin-treated plants was found. Similarly, the activities of catalase, peroxidase, superoxidase dismutase enzymes in leaves of irrigated and melatonin-treated trees were lower than in non-irrigated condition, and superoxide free radial formation was lower in moisture-stressed melatonin-treated trees (0.77 nmol H2O2.mg-1 protein) and irrigated trees (0.65) than moisture-stressed non-treated trees (4.27). Significant variations was found in antioxidants (total, reduced and oxidized glutathione and ascorbate) content and antioxidant enzymes' activities (i.e., glutathione reductase and ascorbate peroxidase) in irrigated, melatonin-treated and non-irrigated conditions. Overall, 150 μM exogenous melatonin applied three times at different fruit development stages may be a sustainable and useful approach to manage transient moisture stress in mango trees thanks to its positive action on the antioxidant system.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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