{"title":"超声波辅助提取 Schrebera swietenioides Roxb.","authors":"Asha Humbal , Laxman Singh , Bhawana Pathak","doi":"10.1016/j.jarmap.2024.100585","DOIUrl":null,"url":null,"abstract":"<div><div><em>Schrebera swietenioides</em> Roxb is an important medicinal plant used for the treatment of various diseases by the indigenous communities. However, this plant is still underexplored in the modern scientific era. Specifically, the leaves of the plant are reported to contain various medicinal properties such as antioxidant, antimicrobial, anti-inflammatory, and antipyretic activity. Herewith, the present work demonstrates and optimizes the extraction process usingan ultrasonic system and comopared the results with conventional extraction methods. Optimization was performed by combining first and second-order polynomial designs at different levels using Plackett-Burman and Central-Composite designs. A total of 31 experiments were conducted at different levels, including ultrasonic power (10–40 %), solvent-to-sample ratio (20–50 mL/g), solvent concentration (50–80 %), and HCl normality (0.2–1 N). The findings of the study suggest a significant (p<0.05) influence of extraction parameters on the extraction yield of the response variables, including total phenol (TPC), total flavonoid (TFC), total tannin (TTC), and the antioxidants activity. Further, the predicted values demonstrated good agreement with the experimental values, exhibiting a high (R<sup>2</sup>>95%) coefficient of determination and a non-significant lack of fit. At the optimized condition, the experimental values of TPC, TFC, TTC and antioxidants (DPPH, ABTS, FRAP) activity were found near the model predicted values. The HPLC-PDA analysis identified ten polyphenolic compounds, among which rutin hydrate (12.991±0.003 mg/g DW), followed by phloridzin (6.660±0.001 mg/g DW), and chlorogenic acid (3.380±0.008 mg/g DW) were the most prominent compounds. Furthermore, the optimized extract showed effective antibacterial activity against <em>Bacillus subtillis</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and <em>Staphylococcus aureus</em> species. In conclusion, the obtained results indicate that the proposed optimization strategy can be utilized to enhance the yield of polyphenolics from the target species with possibility of its application in therapeutics values.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100585"},"PeriodicalIF":3.8000,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultrasonic-assisted extraction of polyphenolics from Schrebera swietenioides Roxb.: An optimization using response surface methodology\",\"authors\":\"Asha Humbal , Laxman Singh , Bhawana Pathak\",\"doi\":\"10.1016/j.jarmap.2024.100585\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Schrebera swietenioides</em> Roxb is an important medicinal plant used for the treatment of various diseases by the indigenous communities. However, this plant is still underexplored in the modern scientific era. Specifically, the leaves of the plant are reported to contain various medicinal properties such as antioxidant, antimicrobial, anti-inflammatory, and antipyretic activity. Herewith, the present work demonstrates and optimizes the extraction process usingan ultrasonic system and comopared the results with conventional extraction methods. Optimization was performed by combining first and second-order polynomial designs at different levels using Plackett-Burman and Central-Composite designs. A total of 31 experiments were conducted at different levels, including ultrasonic power (10–40 %), solvent-to-sample ratio (20–50 mL/g), solvent concentration (50–80 %), and HCl normality (0.2–1 N). The findings of the study suggest a significant (p<0.05) influence of extraction parameters on the extraction yield of the response variables, including total phenol (TPC), total flavonoid (TFC), total tannin (TTC), and the antioxidants activity. Further, the predicted values demonstrated good agreement with the experimental values, exhibiting a high (R<sup>2</sup>>95%) coefficient of determination and a non-significant lack of fit. At the optimized condition, the experimental values of TPC, TFC, TTC and antioxidants (DPPH, ABTS, FRAP) activity were found near the model predicted values. The HPLC-PDA analysis identified ten polyphenolic compounds, among which rutin hydrate (12.991±0.003 mg/g DW), followed by phloridzin (6.660±0.001 mg/g DW), and chlorogenic acid (3.380±0.008 mg/g DW) were the most prominent compounds. Furthermore, the optimized extract showed effective antibacterial activity against <em>Bacillus subtillis</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and <em>Staphylococcus aureus</em> species. In conclusion, the obtained results indicate that the proposed optimization strategy can be utilized to enhance the yield of polyphenolics from the target species with possibility of its application in therapeutics values.</div></div>\",\"PeriodicalId\":15136,\"journal\":{\"name\":\"Journal of Applied Research on Medicinal and Aromatic Plants\",\"volume\":\"43 \",\"pages\":\"Article 100585\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-09-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Research on Medicinal and Aromatic Plants\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214786124000585\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Research on Medicinal and Aromatic Plants","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214786124000585","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Ultrasonic-assisted extraction of polyphenolics from Schrebera swietenioides Roxb.: An optimization using response surface methodology
Schrebera swietenioides Roxb is an important medicinal plant used for the treatment of various diseases by the indigenous communities. However, this plant is still underexplored in the modern scientific era. Specifically, the leaves of the plant are reported to contain various medicinal properties such as antioxidant, antimicrobial, anti-inflammatory, and antipyretic activity. Herewith, the present work demonstrates and optimizes the extraction process usingan ultrasonic system and comopared the results with conventional extraction methods. Optimization was performed by combining first and second-order polynomial designs at different levels using Plackett-Burman and Central-Composite designs. A total of 31 experiments were conducted at different levels, including ultrasonic power (10–40 %), solvent-to-sample ratio (20–50 mL/g), solvent concentration (50–80 %), and HCl normality (0.2–1 N). The findings of the study suggest a significant (p<0.05) influence of extraction parameters on the extraction yield of the response variables, including total phenol (TPC), total flavonoid (TFC), total tannin (TTC), and the antioxidants activity. Further, the predicted values demonstrated good agreement with the experimental values, exhibiting a high (R2>95%) coefficient of determination and a non-significant lack of fit. At the optimized condition, the experimental values of TPC, TFC, TTC and antioxidants (DPPH, ABTS, FRAP) activity were found near the model predicted values. The HPLC-PDA analysis identified ten polyphenolic compounds, among which rutin hydrate (12.991±0.003 mg/g DW), followed by phloridzin (6.660±0.001 mg/g DW), and chlorogenic acid (3.380±0.008 mg/g DW) were the most prominent compounds. Furthermore, the optimized extract showed effective antibacterial activity against Bacillus subtillis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus species. In conclusion, the obtained results indicate that the proposed optimization strategy can be utilized to enhance the yield of polyphenolics from the target species with possibility of its application in therapeutics values.
期刊介绍:
JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.