超声波辅助提取 Schrebera swietenioides Roxb.

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2024-09-29 DOI:10.1016/j.jarmap.2024.100585
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引用次数: 0

摘要

Schrebera swietenioides Roxb 是一种重要的药用植物,被土著社区用于治疗各种疾病。然而,现代科学对这种植物的研究仍然不足。特别是,据报道该植物的叶子含有多种药用特性,如抗氧化、抗菌、消炎和解热活性。本研究利用超声波系统展示并优化了萃取过程,并将结果与传统萃取方法进行了比较。优化方法是使用普拉克特-伯曼(Plackett-Burman)设计和中央-复合设计,在不同水平上结合一阶和二阶多项式设计。共进行了 31 次不同水平的实验,包括超声波功率(10-40%)、溶剂与样品比(20-50 mL/g)、溶剂浓度(50-80%)和盐酸常态(0.2-1 N)。研究结果表明,萃取参数对总酚(TPC)、总黄酮(TFC)、总单宁(TTC)和抗氧化剂活性等响应变量的萃取率有显著影响(p<0.05)。此外,预测值与实验值显示出良好的一致性,显示出较高的决定系数(R2>95%)和非显著的不拟合。在优化条件下,TPC、TFC、TTC 和抗氧化剂(DPPH、ABTS、FRAP)活性的实验值接近模型预测值。HPLC-PDA 分析鉴定出 10 种多酚化合物,其中最主要的化合物是芦丁水合物(12.991±0.003 mg/g DW),其次是叶绿素(6.660±0.001 mg/g DW)和绿原酸(3.380±0.008 mg/g DW)。此外,优化后的提取物对枯草芽孢杆菌、大肠杆菌、绿脓杆菌和金黄色葡萄球菌具有有效的抗菌活性。总之,所获得的结果表明,所提出的优化策略可用于提高目标物种多酚的产量,并有可能将其应用于治疗价值中。
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Ultrasonic-assisted extraction of polyphenolics from Schrebera swietenioides Roxb.: An optimization using response surface methodology
Schrebera swietenioides Roxb is an important medicinal plant used for the treatment of various diseases by the indigenous communities. However, this plant is still underexplored in the modern scientific era. Specifically, the leaves of the plant are reported to contain various medicinal properties such as antioxidant, antimicrobial, anti-inflammatory, and antipyretic activity. Herewith, the present work demonstrates and optimizes the extraction process usingan ultrasonic system and comopared the results with conventional extraction methods. Optimization was performed by combining first and second-order polynomial designs at different levels using Plackett-Burman and Central-Composite designs. A total of 31 experiments were conducted at different levels, including ultrasonic power (10–40 %), solvent-to-sample ratio (20–50 mL/g), solvent concentration (50–80 %), and HCl normality (0.2–1 N). The findings of the study suggest a significant (p<0.05) influence of extraction parameters on the extraction yield of the response variables, including total phenol (TPC), total flavonoid (TFC), total tannin (TTC), and the antioxidants activity. Further, the predicted values demonstrated good agreement with the experimental values, exhibiting a high (R2>95%) coefficient of determination and a non-significant lack of fit. At the optimized condition, the experimental values of TPC, TFC, TTC and antioxidants (DPPH, ABTS, FRAP) activity were found near the model predicted values. The HPLC-PDA analysis identified ten polyphenolic compounds, among which rutin hydrate (12.991±0.003 mg/g DW), followed by phloridzin (6.660±0.001 mg/g DW), and chlorogenic acid (3.380±0.008 mg/g DW) were the most prominent compounds. Furthermore, the optimized extract showed effective antibacterial activity against Bacillus subtillis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus species. In conclusion, the obtained results indicate that the proposed optimization strategy can be utilized to enhance the yield of polyphenolics from the target species with possibility of its application in therapeutics values.
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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