Jun Chang , Ruxuan Zhang , Zhanmeng Fu , Yifan Wang , Jianhui Lei , Junyi Cheng , Caihui Ren , Kunpeng Xu , Chengzhen Gu , Yuanyuan Song , Rensen Zeng , Yuan Qin , Huiying Zhang , Jian Liu
{"title":"OsCNGC7 调节钙动力学并加速水稻叶片衰老","authors":"Jun Chang , Ruxuan Zhang , Zhanmeng Fu , Yifan Wang , Jianhui Lei , Junyi Cheng , Caihui Ren , Kunpeng Xu , Chengzhen Gu , Yuanyuan Song , Rensen Zeng , Yuan Qin , Huiying Zhang , Jian Liu","doi":"10.1016/j.plaphy.2024.109193","DOIUrl":null,"url":null,"abstract":"<div><div>Calcium plays a crucial role in regulating plant senescence. However, the specific effects of increased intranuclear calcium versus cytoplasmic calcium on aging remain unclear. Cyclic nucleotide-gated channels (CNGCs), which manage Ca<sup>2</sup>⁺ levels in plant cells, are particularly significant in this context. These channels are known to relocate between the nuclear envelope and the plasma membrane in response to stress and developmental signals. Through this movement, CNGCs help regulate the balance of cytosolic and intranuclear Ca<sup>2</sup>⁺. In this study, we categorized the 16 CNGC genes in rice into five subgroups. <em>OsCNGCs</em> are notably expressed in leaves, especially during the reproductive stage. Both OsCNGC6 and OsCNGC7 exhibit dual localization to the plasma membrane and the nuclear envelope. Knockdown of <em>OsCNGC7</em> led to reduced levels of Ca<sup>2</sup>⁺ and K⁺ in plants. Conversely, yeast expressing the <em>OsCNGC7</em> gene showed increased sensitivity to Ca<sup>2</sup>⁺. Additionally, while the [Ca<sup>2</sup>⁺]<sub>cyt</sub> was maintained at relatively low levels in both wild-type and <em>OsCNGC7</em>-RNAi lines, the fluorescence intensity was significantly higher in <em>OsCNGC7</em>-overexpressing lines, particularly in the nucleus of root tips. Overexpression of <em>OsCNGC7</em> resulted in enhanced stomatal opening and accelerated leaf senescence from the tillering stage to grain filling in rice. Treatment with MeJA rapidly induced <em>OsCNGC7</em> expression, while knockdown of <em>OsCNGC7</em> delayed both MeJA-induced and dark-induced leaf senescence. Further analysis revealed that OsCNGC7 interacts with OsKAT2 and OsALMT2. In conclusion, our findings highlight the distinct roles of <em>OsCNGCs</em> in regulating senescence. This knowledge could provide new strategies for manipulating plant senescence and enhancing crop productivity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"216 ","pages":"Article 109193"},"PeriodicalIF":6.1000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"OsCNGC7 modulates calcium dynamics and accelerates leaf senescence in rice\",\"authors\":\"Jun Chang , Ruxuan Zhang , Zhanmeng Fu , Yifan Wang , Jianhui Lei , Junyi Cheng , Caihui Ren , Kunpeng Xu , Chengzhen Gu , Yuanyuan Song , Rensen Zeng , Yuan Qin , Huiying Zhang , Jian Liu\",\"doi\":\"10.1016/j.plaphy.2024.109193\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Calcium plays a crucial role in regulating plant senescence. However, the specific effects of increased intranuclear calcium versus cytoplasmic calcium on aging remain unclear. Cyclic nucleotide-gated channels (CNGCs), which manage Ca<sup>2</sup>⁺ levels in plant cells, are particularly significant in this context. These channels are known to relocate between the nuclear envelope and the plasma membrane in response to stress and developmental signals. Through this movement, CNGCs help regulate the balance of cytosolic and intranuclear Ca<sup>2</sup>⁺. In this study, we categorized the 16 CNGC genes in rice into five subgroups. <em>OsCNGCs</em> are notably expressed in leaves, especially during the reproductive stage. Both OsCNGC6 and OsCNGC7 exhibit dual localization to the plasma membrane and the nuclear envelope. Knockdown of <em>OsCNGC7</em> led to reduced levels of Ca<sup>2</sup>⁺ and K⁺ in plants. Conversely, yeast expressing the <em>OsCNGC7</em> gene showed increased sensitivity to Ca<sup>2</sup>⁺. Additionally, while the [Ca<sup>2</sup>⁺]<sub>cyt</sub> was maintained at relatively low levels in both wild-type and <em>OsCNGC7</em>-RNAi lines, the fluorescence intensity was significantly higher in <em>OsCNGC7</em>-overexpressing lines, particularly in the nucleus of root tips. Overexpression of <em>OsCNGC7</em> resulted in enhanced stomatal opening and accelerated leaf senescence from the tillering stage to grain filling in rice. Treatment with MeJA rapidly induced <em>OsCNGC7</em> expression, while knockdown of <em>OsCNGC7</em> delayed both MeJA-induced and dark-induced leaf senescence. Further analysis revealed that OsCNGC7 interacts with OsKAT2 and OsALMT2. In conclusion, our findings highlight the distinct roles of <em>OsCNGCs</em> in regulating senescence. This knowledge could provide new strategies for manipulating plant senescence and enhancing crop productivity.</div></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"216 \",\"pages\":\"Article 109193\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-10-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942824008611\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942824008611","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
OsCNGC7 modulates calcium dynamics and accelerates leaf senescence in rice
Calcium plays a crucial role in regulating plant senescence. However, the specific effects of increased intranuclear calcium versus cytoplasmic calcium on aging remain unclear. Cyclic nucleotide-gated channels (CNGCs), which manage Ca2⁺ levels in plant cells, are particularly significant in this context. These channels are known to relocate between the nuclear envelope and the plasma membrane in response to stress and developmental signals. Through this movement, CNGCs help regulate the balance of cytosolic and intranuclear Ca2⁺. In this study, we categorized the 16 CNGC genes in rice into five subgroups. OsCNGCs are notably expressed in leaves, especially during the reproductive stage. Both OsCNGC6 and OsCNGC7 exhibit dual localization to the plasma membrane and the nuclear envelope. Knockdown of OsCNGC7 led to reduced levels of Ca2⁺ and K⁺ in plants. Conversely, yeast expressing the OsCNGC7 gene showed increased sensitivity to Ca2⁺. Additionally, while the [Ca2⁺]cyt was maintained at relatively low levels in both wild-type and OsCNGC7-RNAi lines, the fluorescence intensity was significantly higher in OsCNGC7-overexpressing lines, particularly in the nucleus of root tips. Overexpression of OsCNGC7 resulted in enhanced stomatal opening and accelerated leaf senescence from the tillering stage to grain filling in rice. Treatment with MeJA rapidly induced OsCNGC7 expression, while knockdown of OsCNGC7 delayed both MeJA-induced and dark-induced leaf senescence. Further analysis revealed that OsCNGC7 interacts with OsKAT2 and OsALMT2. In conclusion, our findings highlight the distinct roles of OsCNGCs in regulating senescence. This knowledge could provide new strategies for manipulating plant senescence and enhancing crop productivity.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.