{"title":"盐度的核心:拟南芥对中性和碱性盐度的不同转录组反应","authors":"","doi":"10.1016/j.envexpbot.2024.105982","DOIUrl":null,"url":null,"abstract":"<div><div>In the context of current climate change, alkaline salinity is increasingly challenging crop yields, especially in arid and semiarid regions. Alkaline salinity is more detrimental to plant performance than neutral salinity and tolerance to neutral salinity may not confer tolerance to alkaline salinity. The mechanisms behind are still poorly understood. This study aims to identify physiological and genetic traits underlying this differential tolerance to neutral and alkaline salinity by exploiting the variation present in natural populations (demes) of <em>Arabidopsis thaliana</em>. Growth, photosynthesis, phytohormone and mineral nutrient profiles, plant water status and transcriptomic changes were analyzed in four demes with contrasting tolerance to neutral and alkaline salinity. Results of this novel holistic approach suggest low internal Fe use efficiency caused by bicarbonate as a driver of enhanced sensitivity to alkaline salinity in plants adapted to neutral salinity prompting photosynthesis inhibition and alteration of the plant’s carbon budget for primary and secondary metabolism. Moreover, alkaline salinity specifically altered the auxin and jasmonic acid signaling pathways, while sustained ABA biosynthesis was an adaptive trait under neutral salinity. Exploring the genes with non-shared expression trends between salinity types, we identified sequence variation at the <em>BGAL4</em> locus associated with advantageous responses to each type of salinity. Weighted correlation network analysis (WGCNA) validated the significant involvement of gene co-expression modules targeted by the enrichment analyses, highlighting the hubs correlated with favorable responses to both salinity types. Overall, the present study points out the complex physiological and genetic mechanisms responsible for plant tolerance to alkaline salinity and proposes target genes for breeding strategies under alkaline saline soils.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"At the core of salinity: Divergent transcriptomic responses to neutral and alkaline salinity in Arabidopsis thaliana\",\"authors\":\"\",\"doi\":\"10.1016/j.envexpbot.2024.105982\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In the context of current climate change, alkaline salinity is increasingly challenging crop yields, especially in arid and semiarid regions. Alkaline salinity is more detrimental to plant performance than neutral salinity and tolerance to neutral salinity may not confer tolerance to alkaline salinity. The mechanisms behind are still poorly understood. This study aims to identify physiological and genetic traits underlying this differential tolerance to neutral and alkaline salinity by exploiting the variation present in natural populations (demes) of <em>Arabidopsis thaliana</em>. Growth, photosynthesis, phytohormone and mineral nutrient profiles, plant water status and transcriptomic changes were analyzed in four demes with contrasting tolerance to neutral and alkaline salinity. Results of this novel holistic approach suggest low internal Fe use efficiency caused by bicarbonate as a driver of enhanced sensitivity to alkaline salinity in plants adapted to neutral salinity prompting photosynthesis inhibition and alteration of the plant’s carbon budget for primary and secondary metabolism. Moreover, alkaline salinity specifically altered the auxin and jasmonic acid signaling pathways, while sustained ABA biosynthesis was an adaptive trait under neutral salinity. Exploring the genes with non-shared expression trends between salinity types, we identified sequence variation at the <em>BGAL4</em> locus associated with advantageous responses to each type of salinity. Weighted correlation network analysis (WGCNA) validated the significant involvement of gene co-expression modules targeted by the enrichment analyses, highlighting the hubs correlated with favorable responses to both salinity types. Overall, the present study points out the complex physiological and genetic mechanisms responsible for plant tolerance to alkaline salinity and proposes target genes for breeding strategies under alkaline saline soils.</div></div>\",\"PeriodicalId\":11758,\"journal\":{\"name\":\"Environmental and Experimental Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental and Experimental Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S009884722400340X\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental and Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S009884722400340X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
在当前气候变化的背景下,碱性盐度对作物产量的挑战越来越大,尤其是在干旱和半干旱地区。与中性盐度相比,碱性盐度更不利于植物生长,而耐受中性盐度未必就能耐受碱性盐度。人们对其背后的机理仍然知之甚少。本研究旨在利用拟南芥自然种群(demes)中存在的变异,找出对中性和碱性盐度的不同耐受性的生理和遗传特征。研究人员分析了对中性和碱性盐度耐受性截然不同的四个种群的生长、光合作用、植物激素和矿质营养概况、植物水分状态和转录组变化。这种新颖的整体方法的结果表明,碳酸氢盐导致的内部铁利用效率低是适应中性盐度的植物对碱性盐度敏感性增强的驱动因素,这促使光合作用受到抑制,并改变了植物初级和次级代谢的碳预算。此外,碱性盐度特异性地改变了辅助素和茉莉酸信号通路,而持续的 ABA 生物合成是中性盐度下的一种适应性特征。在探索盐度类型间非共享表达趋势的基因时,我们发现 BGAL4 基因座上的序列变异与对每种盐度类型的优势反应有关。加权相关网络分析(WGCNA)验证了富集分析所针对的基因共表达模块的显著参与,突出了与对两种盐度类型的有利响应相关的中心。总之,本研究指出了植物耐碱盐度的复杂生理和遗传机制,并提出了碱性盐碱地育种策略的目标基因。
At the core of salinity: Divergent transcriptomic responses to neutral and alkaline salinity in Arabidopsis thaliana
In the context of current climate change, alkaline salinity is increasingly challenging crop yields, especially in arid and semiarid regions. Alkaline salinity is more detrimental to plant performance than neutral salinity and tolerance to neutral salinity may not confer tolerance to alkaline salinity. The mechanisms behind are still poorly understood. This study aims to identify physiological and genetic traits underlying this differential tolerance to neutral and alkaline salinity by exploiting the variation present in natural populations (demes) of Arabidopsis thaliana. Growth, photosynthesis, phytohormone and mineral nutrient profiles, plant water status and transcriptomic changes were analyzed in four demes with contrasting tolerance to neutral and alkaline salinity. Results of this novel holistic approach suggest low internal Fe use efficiency caused by bicarbonate as a driver of enhanced sensitivity to alkaline salinity in plants adapted to neutral salinity prompting photosynthesis inhibition and alteration of the plant’s carbon budget for primary and secondary metabolism. Moreover, alkaline salinity specifically altered the auxin and jasmonic acid signaling pathways, while sustained ABA biosynthesis was an adaptive trait under neutral salinity. Exploring the genes with non-shared expression trends between salinity types, we identified sequence variation at the BGAL4 locus associated with advantageous responses to each type of salinity. Weighted correlation network analysis (WGCNA) validated the significant involvement of gene co-expression modules targeted by the enrichment analyses, highlighting the hubs correlated with favorable responses to both salinity types. Overall, the present study points out the complex physiological and genetic mechanisms responsible for plant tolerance to alkaline salinity and proposes target genes for breeding strategies under alkaline saline soils.
期刊介绍:
Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment.
In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief.
The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB.
The areas covered by the Journal include:
(1) Responses of plants to heavy metals and pollutants
(2) Plant/water interactions (salinity, drought, flooding)
(3) Responses of plants to radiations ranging from UV-B to infrared
(4) Plant/atmosphere relations (ozone, CO2 , temperature)
(5) Global change impacts on plant ecophysiology
(6) Biotic interactions involving environmental factors.