多胺转运和修饰基因簇提高了番茄的耐盐性。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-14 DOI:10.1111/tpj.17074
Jie Yang, Zhonghui Zhang, Xianggui Li, Langchen Guo, Chun Li, Jun Lai, Yige Han, Weizhen Ye, Yuanyuan Miao, Meng Deng, Peng Cao, Yueran Zhang, Xiangyu Ding, Jianing Zhang, Jun Yang, Shouchuang Wang
{"title":"多胺转运和修饰基因簇提高了番茄的耐盐性。","authors":"Jie Yang, Zhonghui Zhang, Xianggui Li, Langchen Guo, Chun Li, Jun Lai, Yige Han, Weizhen Ye, Yuanyuan Miao, Meng Deng, Peng Cao, Yueran Zhang, Xiangyu Ding, Jianing Zhang, Jun Yang, Shouchuang Wang","doi":"10.1111/tpj.17074","DOIUrl":null,"url":null,"abstract":"<p><p>Polyamines act as protective compounds directly protecting plants from stress-related damage, while also acting as signaling molecules to participate in serious abiotic stresses. However, the molecular mechanisms underlying these effects are poorly understood. Here, we utilized metabolome genome-wide association study to investigate the polyamine content of wild and cultivated tomato accessions, and we discovered a new gene cluster that drove polyamine content during tomato domestication. The gene cluster contains two polyphenol oxidases (SlPPOE and SlPPOF), two BAHD acyltransferases (SlAT4 and SlAT5), a coumaroyl-CoA ligase (Sl4CL6), and a polyamine uptake transporter (SlPUT3). SlPUT3 mediates polyamine uptake and transport, while the five other genes are involved in polyamine modification. Further salt tolerance assays demonstrated that SlPPOE, SlPPOF, and SlAT5 overexpression lines showed greater phenolamide accumulation and salt tolerance as compared with wild-type (WT). Meanwhile, the exogenous application of Spm to SlPUT3-OE lines displayed salt tolerance compared with WT, while having the opposite effect in slput3 lines, confirms that the polyamine and phenolamide can play a protective role by alleviating cell damage. SlPUT3 interacted with SlPIP2;4, a H<sub>2</sub>O<sub>2</sub> transport protein, to maintain H<sub>2</sub>O<sub>2</sub> homeostasis. Polyamine-derived H<sub>2</sub>O<sub>2</sub> linked Spm to stress responses, suggesting that Spm signaling activates stress response pathways. Collectively, our finding reveals that the H<sub>2</sub>O<sub>2</sub>-polyamine-phenolamide module coordinately enhanced tomato salt stress tolerance and provide a foundation for tomato stress-resistance breeding.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A gene cluster for polyamine transport and modification improves salt tolerance in tomato.\",\"authors\":\"Jie Yang, Zhonghui Zhang, Xianggui Li, Langchen Guo, Chun Li, Jun Lai, Yige Han, Weizhen Ye, Yuanyuan Miao, Meng Deng, Peng Cao, Yueran Zhang, Xiangyu Ding, Jianing Zhang, Jun Yang, Shouchuang Wang\",\"doi\":\"10.1111/tpj.17074\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Polyamines act as protective compounds directly protecting plants from stress-related damage, while also acting as signaling molecules to participate in serious abiotic stresses. However, the molecular mechanisms underlying these effects are poorly understood. Here, we utilized metabolome genome-wide association study to investigate the polyamine content of wild and cultivated tomato accessions, and we discovered a new gene cluster that drove polyamine content during tomato domestication. The gene cluster contains two polyphenol oxidases (SlPPOE and SlPPOF), two BAHD acyltransferases (SlAT4 and SlAT5), a coumaroyl-CoA ligase (Sl4CL6), and a polyamine uptake transporter (SlPUT3). SlPUT3 mediates polyamine uptake and transport, while the five other genes are involved in polyamine modification. Further salt tolerance assays demonstrated that SlPPOE, SlPPOF, and SlAT5 overexpression lines showed greater phenolamide accumulation and salt tolerance as compared with wild-type (WT). Meanwhile, the exogenous application of Spm to SlPUT3-OE lines displayed salt tolerance compared with WT, while having the opposite effect in slput3 lines, confirms that the polyamine and phenolamide can play a protective role by alleviating cell damage. SlPUT3 interacted with SlPIP2;4, a H<sub>2</sub>O<sub>2</sub> transport protein, to maintain H<sub>2</sub>O<sub>2</sub> homeostasis. Polyamine-derived H<sub>2</sub>O<sub>2</sub> linked Spm to stress responses, suggesting that Spm signaling activates stress response pathways. Collectively, our finding reveals that the H<sub>2</sub>O<sub>2</sub>-polyamine-phenolamide module coordinately enhanced tomato salt stress tolerance and provide a foundation for tomato stress-resistance breeding.</p>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2024-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://doi.org/10.1111/tpj.17074\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/tpj.17074","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

多胺是一种保护性化合物,可直接保护植物免受与胁迫相关的损害,同时还可作为信号分子参与严重的非生物胁迫。然而,人们对这些作用的分子机制知之甚少。在此,我们利用代谢组全基因组关联研究调查了野生番茄和栽培番茄的多胺含量,并发现了一个在番茄驯化过程中驱动多胺含量的新基因簇。该基因簇包含两个多酚氧化酶(SlPPOE和SlPPOF)、两个BAHD酰基转移酶(SlAT4和SlAT5)、一个香豆酰-CoA连接酶(Sl4CL6)和一个多胺吸收转运体(SLPUT3)。SlPUT3 介导多胺的吸收和转运,而其他五个基因则参与多胺的修饰。进一步的耐盐性实验表明,与野生型(WT)相比,SlPPOE、SlPPOF 和 SlAT5 过表达株表现出更高的苯甲酰胺积累和耐盐性。同时,与 WT 相比,SlPUT3-OE 株系外源施用 Spm 会表现出耐盐性,而 slput3 株系则相反,这证实了多胺和苯酚酰胺可以通过减轻细胞损伤发挥保护作用。SlPUT3 与 H2O2 转运蛋白 SlPIP2;4 相互作用,维持 H2O2 平衡。多胺衍生的 H2O2 将 Spm 与应激反应联系起来,表明 Spm 信号激活了应激反应途径。总之,我们的发现揭示了H2O2-多胺-苯酚酰胺模块协调地增强了番茄对盐胁迫的耐受性,为番茄抗逆育种提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
A gene cluster for polyamine transport and modification improves salt tolerance in tomato.

Polyamines act as protective compounds directly protecting plants from stress-related damage, while also acting as signaling molecules to participate in serious abiotic stresses. However, the molecular mechanisms underlying these effects are poorly understood. Here, we utilized metabolome genome-wide association study to investigate the polyamine content of wild and cultivated tomato accessions, and we discovered a new gene cluster that drove polyamine content during tomato domestication. The gene cluster contains two polyphenol oxidases (SlPPOE and SlPPOF), two BAHD acyltransferases (SlAT4 and SlAT5), a coumaroyl-CoA ligase (Sl4CL6), and a polyamine uptake transporter (SlPUT3). SlPUT3 mediates polyamine uptake and transport, while the five other genes are involved in polyamine modification. Further salt tolerance assays demonstrated that SlPPOE, SlPPOF, and SlAT5 overexpression lines showed greater phenolamide accumulation and salt tolerance as compared with wild-type (WT). Meanwhile, the exogenous application of Spm to SlPUT3-OE lines displayed salt tolerance compared with WT, while having the opposite effect in slput3 lines, confirms that the polyamine and phenolamide can play a protective role by alleviating cell damage. SlPUT3 interacted with SlPIP2;4, a H2O2 transport protein, to maintain H2O2 homeostasis. Polyamine-derived H2O2 linked Spm to stress responses, suggesting that Spm signaling activates stress response pathways. Collectively, our finding reveals that the H2O2-polyamine-phenolamide module coordinately enhanced tomato salt stress tolerance and provide a foundation for tomato stress-resistance breeding.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
期刊最新文献
Development of Aegilops comosa and Aegilops caudata-specific molecular markers and fluorescence in situ hybridization probes based on specific-locus amplified fragment sequencing. Laser dissection-assisted phloem transcriptomics highlights the metabolic and physiological changes accompanying clubroot disease progression in oilseed rape. Disruption of aldehyde dehydrogenase decreases cell wall-bound p-hydroxycinnamates and improves cell wall digestibility in rice. Oxidation of four monoterpenoid indole alkaloid classes by three cytochrome P450 monooxygenases from Tabernaemontana litoralis. RMI1 is essential for maintaining rice genome stability at high temperature.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1