补充琥珀酸对大黄鱼幼体生长性能、消化能力、肠道发育和免疫力的影响

IF 4.1 2区 农林科学 Q1 FISHERIES Fish & shellfish immunology Pub Date : 2024-10-17 DOI:10.1016/j.fsi.2024.109972
Chenxiang Zhang , Yongtao Liu , Zhijie Shi , Chuanwei Yao , Jianmin Zhang , Yuntao Wang , Jiahui Liu , Kangsen Mai , Qinghui Ai
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引用次数: 0

摘要

人工微量饲料的应用是提高和规范水生动物幼体质量的有效途径。然而,由于幼体的利用能力有限,微量饲料的广泛应用面临瓶颈。为了研究日粮琥珀酸(SA)对大黄鱼幼体(初始体重 11.33 ± 0.57 mg)生长性能、消化能力、肠道发育和免疫力的影响,我们进行了为期 30 天的饲养实验。分别添加 0.00%、0.01%、0.02% 和 0.03% 的 SA,配制了四种等氮和离脂日粮。结果表明,添加 0.02% SA 的日粮能显著提高幼虫的最终体重和特定生长率。在消化能力方面,添加 0.01% SA 能显著提高肠道和胰腺中胰蛋白酶的活性。此外,添加 0.01% SA 能显著提高肠道部分的淀粉酶活性,而添加 0.01%-0.02% SA 的日粮则能显著提高胰腺部分的脂肪酶活性。在肠道发育方面,添加 0.01% SA 能显著提高肠道刷状缘膜上碱性磷酸酶和亮氨酸氨肽酶的活性。此外,补充 0.03% 的 SA 还能显著提高闭塞素的表达。在免疫方面,与对照组相比,饲喂 0.01%-0.02% SA 日粮的幼虫溶菌酶活性明显提高。添加 0.01% SA 还能显著提高 iNOS 活性和 NO 含量。综上所述,本研究结果表明,添加 0.02% SA 可通过提高消化酶活性、促进肠道发育和增强非特异性免疫力来改善大黄鱼幼体的生长性能。
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Effects of dietary succinic acid supplementation on growth performance, digestive ability, intestinal development and immunity of large yellow croaker (Larimichthys crocea) larvae
The application of artificial micro-diet is an effective way to improve and standardize the quality of aquatic animal larvae. However, the widespread adoption of micro-diet faces a bottleneck due to the limited utilization capacity of the larvae. A 30-day feeding experiment was carried out to investigate the effect of dietary succinic acid (SA) on the growth performance, digestive ability, intestinal development, and immunity of large yellow croaker larvae (initial body weight 11.33 ± 0.57 mg). Four isonitrogenous and isolipidic diets were formulated, incorporating 0.00 %, 0.01 %, 0.02 % and 0.03 % SA separately. The results showed that a diet with 0.02 % SA significantly increased both the final body weight and the specific growth rate of the larvae. Regarding digestive ability, 0.01 % SA supplementation significantly enhanced trypsin activity in both intestinal and pancreatic segments. In addition, 0.01 % SA supplementation notably improved amylase activity in the intestinal segment, while diets with 0.01%–0.02 % SA significantly improved lipase activity in the pancreatic segment. In terms of intestinal development, 0.01 % SA supplementation remarkably boosted the activities of alkaline-phosphatase and leucine-aminopeptidase on brush border membrane in intestine. Furthermore, 0.03 % SA supplementation significantly increased the expression of occludin. In terms of immunity, larvae fed diets with 0.01%–0.02 % SA exhibited significantly higher lysozyme activity compared to the control group. Supplementation with 0.01 % SA also significantly increased both iNOS activity and NO content. In summary, the findings of this study suggested that supplementing 0.02 % SA can improve the growth performance of large yellow croaker larvae by improving digestive enzymes activities, promoting intestinal development, and enhancing nonspecific immunity.
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来源期刊
Fish & shellfish immunology
Fish & shellfish immunology 农林科学-海洋与淡水生物学
CiteScore
7.50
自引率
19.10%
发文量
750
审稿时长
68 days
期刊介绍: Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.
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