曼氏血吸虫感染小鼠乳斑中的嗜酸性粒细胞和 B 细胞动态--与脾脏和骨髓以及髓外嗜酸性粒细胞生成的比较。

IF 4.8 4区 医学 Q2 IMMUNOLOGY International immunology Pub Date : 2024-10-18 DOI:10.1093/intimm/dxae064
Bruno Marques Vieira, Beatriz Fernandes Almeida, Marcelo Pelajo Machado
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引用次数: 0

摘要

乳斑是在人类和其他脊椎动物的网膜中发现的结构,是与腹腔相关的淋巴细胞组织的一部分。它们主要由 B 淋巴细胞、T 淋巴细胞和巨噬细胞组成。此外,还有少量间皮细胞、基质细胞、树突状细胞和罕见的肥大细胞。在曼氏血吸虫感染的实验模型中,网膜和乳斑被明显激活,出现大量嗜酸性粒细胞。尽管嗜酸性粒细胞已被描述多年,但乳斑中发现的细胞的完整特征及其功能在很大程度上仍未被探索。在这里,我们评估了乳斑中白细胞群的平衡状态以及曼氏血吸虫感染的小鼠模型。组织病理学特征、表型轮廓分析以及嗜酸性粒细胞祖细胞和前体细胞的嗜酸性粒细胞潜能特征显示,嗜酸性粒细胞祖细胞和前体细胞与脾脏和骨髓进行比较后发现,在感染小鼠体内,嗜酸性粒细胞乳斑显著活化,其轮廓在分析时间内发生了变化,显示出嗜酸性粒细胞迁移和活化的迹象,并伴有局部嗜酸性粒细胞生成和嗜酸性粒细胞群的维持。在幼稚小鼠体内,B1a 和 B1b 细胞只占 B 淋巴细胞的一小部分。然而,B1b 细胞在感染期间会显著增大,在 60 DPI 时达到峰值,到 90 DPI 时趋于稳定。B1a 细胞最初也会增加,但随着时间的推移会减少。乳斑的表现不同于其他初级和次级淋巴器官,它是空腔免疫的核心淋巴器官。
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Eosinophil and B cell dynamics in the milky spots from Schistosoma mansoni-infected mice - Comparison with spleen and bone marrow, and extramedullary eosinopoiesis.

The milky spots are structures found in the omentum of humans and other vertebrates, representing a fraction of the lymphomyeloid tissue associated with the celom. They majorly consist of B lymphocytes, T lymphocytes, and macrophages. Also found in smaller quantities are mesothelial, stromal, dendritic, and rare mast cells. In an experimental model of Schistosoma mansoni infection, there is significant activation of the omentum and milky spots, which exhibit numerous eosinophils. Despite being described for many years, the complete profile of cells found in milky spots and their functions remains largely unexplored. Here, we evaluate the leukocyte populations of the milky spots in homeostasis and a murine model of Schistosoma mansoni infection. The histopathological characterizations, phenotypic profile analysis, and characterization of the eosinophilic potential of progenitors and precursors comparing the milky spots with the spleen and bone marrow showed significant activation of milky spots in infected mice, with changes in the profile over the analyzed times, showing signs of migration and activation of eosinophils, with local eosinopoiesis and maintenance of the eosinophilic population. In naive mice, B1a and B1b cells comprise only a small fraction of B lymphocytes. However, B1b cells expand significantly during infection, peaking at 60 DPI before stabilizing by 90 DPI. B1a cells also increase initially but decrease over time. The behavior of milky spots differs from other primary and secondary lymphoid organs, acting as a central lymphoid organ in cavity immunity.

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来源期刊
International immunology
International immunology 医学-免疫学
CiteScore
9.30
自引率
2.30%
发文量
51
审稿时长
6-12 weeks
期刊介绍: International Immunology is an online only (from Jan 2018) journal that publishes basic research and clinical studies from all areas of immunology and includes research conducted in laboratories throughout the world.
期刊最新文献
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