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The tryptophan metabolic pathway of the microbiome and host cells in health and disease. 微生物组和宿主细胞在健康和疾病中的色氨酸代谢途径。
IF 5.4 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-11-14 DOI: 10.1093/intimm/dxae035
Kentaro Miyamoto, Tomohisa Sujino, Takanori Kanai

The intricate and dynamic tryptophan (Trp) metabolic pathway in both the microbiome and host cells highlights its profound implications for health and disease. This pathway involves complex interactions between host cellular and bacteria processes, producing bioactive compounds such as 5-hydroxytryptamine (5-HT) and kynurenine derivatives. Immune responses to Trp metabolites through specific receptors have been explored, highlighting the role of the aryl hydrocarbon receptor in inflammation modulation. Dysregulation of this pathway is implicated in various diseases, such as Alzheimer's and Parkinson's diseases, mood disorders, neuronal diseases, autoimmune diseases such as multiple sclerosis (MS), and cancer. In this article, we describe the impact of the 5-HT, Trp, indole, and Trp metabolites on health and disease. Furthermore, we review the impact of microbiome-derived Trp metabolites that affect immune responses and contribute to maintaining homeostasis, especially in an experimental autoimmune encephalitis model of MS.

微生物组和宿主细胞中错综复杂而又充满活力的色氨酸(Trp)代谢途径凸显了其对健康和疾病的深远影响。这一途径涉及宿主细胞和细菌过程之间复杂的相互作用,产生生物活性化合物,如 5-羟色胺(5-HT)和犬尿氨酸(Kyn)衍生物。通过特定受体对 Trp 代谢物的免疫反应已得到探讨,其中突出了芳基烃受体(AHR)在炎症调节中的作用。这一途径的失调与多种疾病有关,如阿尔茨海默氏症和帕金森氏症、情绪障碍、神经元疾病、自身免疫性疾病(如多发性硬化症)和癌症。本文介绍了 5-羟色胺、Trp、吲哚和 Trp 代谢物对健康和疾病的影响。此外,我们还回顾了微生物衍生的 Trp 代谢物对免疫反应的影响以及对维持体内平衡的作用,尤其是在多发性硬化症的实验性自身免疫性脑炎 (EAE) 模型中的作用。
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引用次数: 0
Supersulphides suppress type-I and type-II interferon responses by blocking JAK/STAT signalling in macrophages. 超硫化物通过阻断巨噬细胞中的 JAK/STAT 信号传导抑制Ⅰ型和Ⅱ型干扰素反应
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-11-14 DOI: 10.1093/intimm/dxae040
Xiaoyan Li, Touya Toyomoto, Tianli Zhang, Chunyu Guo, Stephen Lindahl, Hiroyasu Tsutsuki, Ming Xian, Tomohiro Sawa

Interferons (IFNs) are cytokines produced and secreted by immune cells when viruses, tumour cells, and so forth, invade the body. Their biological effects are diverse, including antiviral, cell growth-inhibiting, and antitumour effects. The main subclasses of IFNs include type-I (e.g. IFN-α and IFN-β) and type-II (IFN-γ), which activate intracellular signals by binding to type-I and type-II IFN receptors, respectively. We have previously shown that when macrophages are treated with supersulphide donors, which have polysulphide structures in which three or more sulphur atoms are linked within the molecules, IFN-β-induced cellular responses, including signal transducer and activator of transcription 1 (STAT1) phosphorylation and inducible nitric oxide synthase (iNOS) expression, were strongly suppressed. However, the subfamily specificity of the suppression of IFN signals by supersulphides and the mechanism of this suppression are unknown. This study demonstrated that supersulphide donor N-acetyl-L-cysteine tetrasulphide (NAC-S2) can inhibit IFN signalling in macrophages stimulated not only with IFN-α/β but also with IFN-γ. Our data suggest that NAC-S2 blocks phosphorylation of Janus kinases (JAKs), thereby contributing to the inhibition of phosphorylation of STAT1. Under the current experimental conditions, the hydrogen sulphide (H2S) donor NaHS failed to inhibit IFN signalling. Similar to NAC-S2, the carbohydrate-based supersulphide donor thioglucose tetrasulphide (TGS4) was capable of strongly inhibiting tumour necrosis factor-α production, iNOS expression, and nitric oxide production from macrophages stimulated with lipopolysaccharide. Further understanding of the molecular mechanisms by which supersulphide donors exhibit their inhibitory actions towards JAK/STAT signalling is a necessary basis for the development of supersulphide-based therapeutic strategy against autoimmune disorders with dysregulated IFN signalling.

干扰素(IFNs)是免疫细胞在病毒、肿瘤细胞等入侵人体时产生和分泌的细胞因子。其生物效应多种多样,包括抗病毒、抑制细胞生长和抗肿瘤作用。干扰素的主要亚类包括 I 型(如 IFN-α 和 IFN-β)和 II 型(IFN-γ),它们分别通过与 I 型和 II 型 IFN 受体结合来激活细胞内信号。我们以前的研究表明,当用超硫化物供体处理巨噬细胞时,IFN-β 诱导的细胞反应,包括信号转导和激活转录 1(STAT1)磷酸化和诱导型一氧化氮合酶(iNOS)的表达,都会受到强烈抑制。然而,超硫化物抑制 IFN 信号的亚家族特异性及其抑制机制尚不清楚。本研究表明,超硫化物供体 N-乙酰-L-半胱氨酸四硫化物(NAC-S2)不仅能抑制 IFN-α/β 刺激巨噬细胞中的 IFN 信号,还能抑制 IFN-γ 刺激巨噬细胞中的 IFN 信号。我们的数据表明,NAC-S2 可阻断 Janus 激酶(JAKs)的磷酸化,从而有助于抑制 STAT1 的磷酸化。在目前的实验条件下,硫化氢(H2S)供体 NaHS 未能抑制 IFN 信号转导。与 NAC-S2 类似,基于碳水化合物的硫化氢供体硫代葡萄糖四硫化物(TGS4)也能强烈抑制受脂多糖刺激的巨噬细胞产生肿瘤坏死因子-α、iNOS 表达和一氧化氮。进一步了解超硫化物供体如何对 JAK/STAT 信号转导产生抑制作用的分子机制,是开发基于超硫化物的治疗策略以防治 IFN 信号转导失调的自身免疫性疾病的必要基础。
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引用次数: 0
γδ intraepithelial lymphocytes acquire the ability to produce IFN-γ in a different time course than αβ intraepithelial lymphocytes. γδ上皮内淋巴细胞获得产生 IFN-γ 的能力的时间过程与 αβ 上皮内淋巴细胞不同。
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-11-14 DOI: 10.1093/intimm/dxae034
Shizue Tani-Ichi, Koichi Ikuta

An age-dependent increase in interferon (IFN)-γ expression by intestinal intraepithelial lymphocytes (IELs) contributes to the acquisition of resistance to infection by pathogens. However, how IELs acquire the ability to produce IFN-γ remains to be elucidated. Here, we report that IELs in the small intestine acquire the ability to rapidly produce IFN-γ at two distinct life stages. TCRαβ+ IELs (αβIELs) started producing IFN-γ at 4 weeks of age, within 1 week after weaning. In contrast, TCRγδ+ IELs (γδIELs) started producing IFN-γ at 7 weeks of age. In mice lacking Eγ4, an enhancer of the TCRγ locus (Eγ4-/- mice), Thy-1+ Vγ5+ γδIELs, a major subpopulation of γδIELs, were specifically reduced and their ability to produce IFN-γ was severely impaired, whereas Vγ2+ γδIELs normally produced IFN-γ. In Eγ4-/- mice, TCR expression levels were reduced in Vγ5+ γδIEL precursors in the thymus but unchanged in the Vγ5+ IELs. Nevertheless, TCR responsiveness in Vγ5+ γδIELs was impaired in Eγ4-/- mice, suggesting that the TCR signal received in the thymus may determine TCR responsiveness and the ability to produce IFN-γ in the gut. These results suggest that αβIELs and γδIELs start producing IFN-γ at different life stages and that the ability of Vγ5+ γδIELs to produce IFN-γ in the gut may be predetermined by TCR signalling in IEL precursors in the thymus.

肠上皮内淋巴细胞(IELs)表达的 IFN-γ 随年龄增长而增加,这有助于获得抵抗病原体感染的能力。然而,IELs如何获得产生IFN-γ的能力仍有待阐明。在这里,我们报告了小肠中的 IELs 在两个不同的生命阶段获得快速产生 IFN-γ 的能力。TCRαβ+ IELs(αβIELs)在4周龄时,即断奶后1周内开始产生IFN-γ。相反,TCRγδ+ IELs(γδIELs)在 7 周龄时开始产生 IFN-γ。在缺乏 TCRγ 基因座增强子 Eγ4 的小鼠(Eγ4-/- 小鼠)中,Thy-1+ Vγ5+ γδIELs (γδIELs 的一个主要亚群)特异性减少,其产生 IFN-γ 的能力严重受损,而 Vγ2+ γδIELs 则正常产生 IFN-γ。在 Eγ4-/- 小鼠中,胸腺中 Vγ5+ γδIEL 前体的 TCR 表达水平降低,但 Vγ5+ IELs 的表达水平不变。然而,在 Eγ4-/- 小鼠中,Vγ5+ γδIEL 的 TCR 反应性受损,这表明胸腺中接收的 TCR 信号可能决定了 TCR 反应性和在肠道中产生 IFN-γ 的能力。这些结果表明,αβIELs 和 γδIELs 在不同的生命阶段开始产生 IFN-γ,Vγ5+ γδIELs 在肠道中产生 IFN-γ 的能力可能是由胸腺中 IEL 前体的 TCR 信号决定的。
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引用次数: 0
Synchronized development of thymic eosinophils and thymocytes. 胸腺嗜酸性粒细胞和胸腺细胞的同步发育
IF 5.4 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-11-14 DOI: 10.1093/intimm/dxae037
Ayami Ota, Takahiro Iguchi, Sachiko Nitta, Ryunosuke Muro, Nanami Mino, Masayuki Tsukasaki, Josef M Penninger, Takeshi Nitta, Hiroshi Takayanagi

The thymus is an organ required for T cell development and is also an eosinophil-rich organ; however, the nature and function of thymic eosinophils remain unclear. Here, we characterized the gene expression and differentiation mechanism of thymic eosinophils in mice. Thymic eosinophils showed a distinct gene expression profile compared with other organ-resident eosinophils. The number of thymic eosinophils was controlled by medullary thymic epithelial cells (mTECs). In Rag-deficient mice, the unique gene expression signature of thymic eosinophils was lost but restored by pre-T cell receptor signalling, which induces CD4+ CD8+ thymocyte differentiation, indicating that T cell differentiation beyond the CD4- CD8- stage is necessary and sufficient for the induction of thymic eosinophils. These results demonstrate that thymic eosinophils are quantitatively and qualitatively regulated by mTECs and developing thymocytes, respectively, suggesting that thymic eosinophils are a distinct, thymus-specific cell subset, induced by interactions with thymic cells.

胸腺是 T 细胞发育所需的器官,也是嗜酸性粒细胞丰富的器官;然而,胸腺嗜酸性粒细胞的性质和功能仍不清楚。在这里,我们研究了小鼠胸腺嗜酸性粒细胞的基因表达和分化机制。与其他器官驻留的嗜酸性粒细胞相比,胸腺嗜酸性粒细胞显示出独特的基因表达谱。胸腺嗜酸性粒细胞的数量由胸腺髓质上皮细胞控制。在 Rag 缺失的小鼠中,胸腺嗜酸性粒细胞的独特基因表达特征消失了,但通过诱导 CD4+ CD8+ 胸腺细胞分化的前 T 细胞受体信号恢复了这一特征。这些结果表明,胸腺嗜酸性粒细胞在数量和质量上分别受到髓质胸腺上皮细胞和发育中胸腺细胞的调控,这表明胸腺嗜酸性粒细胞是一种独特的胸腺特异性细胞亚群,是通过与胸腺细胞相互作用诱导的。
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引用次数: 0
Regulation of memory CD4+ T-cell generation by intrinsic and extrinsic IL-27 signaling during malaria infection. 在疟疾感染过程中,IL-27 的内在和外在信号调节记忆 CD4+ T 细胞的生成。
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-11-14 DOI: 10.1093/intimm/dxae039
Sanjaadorj Tsogtsaikhan, Shin-Ichi Inoue, Ganchimeg Bayarsaikhan, Maria Lourdes Macalinao, Daisuke Kimura, Mana Miyakoda, Masahiro Yamamoto, Hiromitsu Hara, Hiroki Yoshida, Katsuyuki Yui

The generation and maintenance of memory T cells are regulated by various factors, including cytokines. Previous studies have shown that IL-27 is produced during the early acute phase of Plasmodium chabaudi chabaudi AS (Pcc) infection and inhibits the development of Th1-type memory CD4+ T cells. However, whether IL-27 acts directly on its receptor on Plasmodium-specific CD4+ T cells or indirectly via its receptor on other immune cells remains unclear. We aimed to determine the role of IL-27 receptor signaling in different immune cell types in regulating the generation and phenotype of memory CD4+ T cells during Plasmodium infection. We utilized Plasmodium-specific T-cell antigen receptor (TCR) transgenic mice, PbT-II, and Il27rα-/- mice to assess the direct and indirect effects of IL-27 signaling on memory CD4+ T-cell generation. Mice were transferred with PbT-II or Il27rα-/- PbT-II cells and infected with Pcc. Conditional knockout mice lacking the IL-27 receptor in T cells or dendritic cells were employed to discern the specific immune cell types involved in IL-27 receptor signaling. High levels of memory in PbT-II cells with Th1-shift occurred only when both PbT-II and host cells lacked the IL-27 receptor, suggesting the predominant inhibitory role of IL-27 signaling in both cell types. Furthermore, IL-27 receptor signaling in T cells limited the number of memory CD4+ T cells, while signaling in both T and dendritic cells contributed to the Th1 dominance of memory CD4+ T cells. These findings underscore the complex cytokine signaling network regulating memory CD4+ T cells during Plasmodium infection.

记忆 T 细胞的生成和维持受多种因素(包括细胞因子)的调控。先前的研究表明,IL-27 在沙鲍迪疟原虫(Pcc)感染的早期急性期产生,并抑制 Th1 型记忆 CD4+ T 细胞的发育。然而,IL-27是直接作用于其在疟原虫特异性CD4+ T细胞上的受体,还是间接通过其在其他免疫细胞上的受体发挥作用,目前仍不清楚。我们的目的是确定在疟原虫感染期间,IL-27 受体信号在不同免疫细胞类型中调节记忆 CD4+ T 细胞的生成和表型的作用。我们利用疟原虫特异性 TCR 转基因小鼠 PbT-II 和 Il27rα-/- 小鼠来评估 IL-27 信号传导对记忆 CD4+ T 细胞生成的直接和间接影响。用 PbT-II 或 Il27rα-/- PbT-II 细胞转移小鼠并用 Pcc 感染。利用T细胞或树突状细胞中缺乏IL-27受体的条件性基因敲除小鼠来确定参与IL-27受体信号转导的特定免疫细胞类型。只有当 PbT-II 细胞和宿主细胞都缺乏 IL-27 受体时,PbT-II 细胞才会出现高水平的 Th1 转移记忆,这表明 IL-27 信号在两种细胞类型中都起着主要的抑制作用。此外,T细胞中的IL-27受体信号限制了记忆CD4+ T细胞的数量,而T细胞和树突状细胞中的信号则导致记忆CD4+ T细胞的Th1优势。这些发现强调了在疟原虫感染期间调节记忆CD4+ T细胞的复杂细胞因子信号网络。
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引用次数: 0
The role of polyreactive memory B cells in systemic lupus erythematosus. 多反应记忆 B 细胞在系统性红斑狼疮中的作用。
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-11-08 DOI: 10.1093/intimm/dxae058
Keishi Fujio, Toshiyuki Ushijima, Tomohisa Okamura, Mineto Ota

In systemic lupus erythematosus (SLE), the production of autoantibodies is a crucial characteristic, and B cells play a significant role in its pathogenesis. B cells are the immune cells most associated with the genetic predispositions of SLE, and recent clinical studies showing that anti-CD19 CAR-T cell therapy induces drug-free remission have underscored the importance of B cells in SLE. Meanwhile, various B cell subsets exist across different stages of differentiation, from naive B cells to plasma-cells, and identifying the important subpopulations within SLE remains a critical future challenge. Years of B cell repertoire analyses have revealed the importance of polyreactive B cell receptors (BCRs) and autoantibodies that react to a various self-antigens and microbial antigens. Particularly, memory B cells with polyreactive BCRs, which play a crucial role in biological defense during the fetal stage, are characteristically differentiated in SLE. Type I interferon-mediated expression of CXCL13 and IL21 in CD4+ T cells is associated with the development of polyreactive memory B cells. The expansion of the polyreactive B cell repertoire, vital for defending against infections such as viruses, may exert an intrinsic function in SLE.

在系统性红斑狼疮(SLE)中,自身抗体的产生是一个重要特征,而B细胞在其发病机制中扮演着重要角色。B细胞是与系统性红斑狼疮遗传倾向最相关的免疫细胞,最近的临床研究显示,抗CD19 CAR-T细胞疗法可诱导无药缓解,这凸显了B细胞在系统性红斑狼疮中的重要性。同时,从天真 B 细胞到浆细胞,各种 B 细胞亚群存在于不同的分化阶段,鉴别系统性红斑狼疮中的重要亚群仍是未来的关键挑战。多年的 B 细胞谱系分析显示,多反应性 B 细胞受体(BCR)和自身抗体对各种自身抗原和微生物抗原的反应非常重要。特别是,具有多反应性BCR的记忆B细胞在胎儿阶段的生物防御中起着至关重要的作用,而在系统性红斑狼疮中,记忆B细胞的分化具有特征性。I 型干扰素介导的 CD4+ T 细胞中 CXCL13 和 IL21 的表达与多反应性记忆 B 细胞的发育有关。多反应性 B 细胞扩增对抵御病毒等感染至关重要,可能在系统性红斑狼疮中发挥着内在功能。
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引用次数: 0
The role of dendritic cells in the instruction of helper T cells in the allergic march. 树突状细胞在过敏前行过程中对辅助性 T 细胞的指导作用。
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-10-26 DOI: 10.1093/intimm/dxae050
Masato Kubo, Yasuyo Harada, Takanori Sasaki

Allergy is a complex array of diseases influenced by innate and adaptive immunity, genetic polymorphisms, and environmental triggers. Atopic dermatitis is a chronic inflammatory skin disease characterized by barrier defects and immune dysregulation, sometimes leading to asthma and food allergies because of the atopic march. During atopic skin inflammation, Langerhans cells and dendritic cells (DCs) in the skin capture and deliver allergen information to local lymph nodes. DCs are essential immune sensors coordinating immune reactions by capturing and presenting antigens to T cells. In the context of allergic responses, DCs play a crucial role in instructing two types of helper T cells-type 2 helper T (Th2) cells and follicular helper T (TFH) cells-in allergic responses and IgE antibody responses. In skin sensitization, the differentiation and function of Th2 cells and TFH cells are influenced by skin-derived factors, including epithelial cytokines, chemokines, and signalling pathways to modify the function of migratory DCs and conventional DCs. In this review, we aim to understand the specific mechanisms involving DCs in allergic responses to provide insights into the pathogenesis of allergic diseases and potential therapeutic strategies.

过敏是受先天性免疫和适应性免疫、基因多态性和环境诱因影响的一系列复杂疾病。特应性皮炎(AD)是一种慢性炎症性皮肤病,其特点是屏障缺陷和免疫调节失调,有时会因特应性进展而导致哮喘和食物过敏。在特应性皮肤炎症期间,皮肤中的朗格汉斯细胞和树突状细胞(DC)会捕捉过敏原信息并将其传递到局部淋巴结。DC 是重要的免疫传感器,通过捕捉抗原并将其呈现给 T 细胞来协调免疫反应。在过敏反应中,DC 在指导两种辅助性 T 细胞(2 型辅助性 T 细胞(Th2)和滤泡辅助性 T 细胞(TFH))的过敏反应和 IgE 抗体反应中发挥着至关重要的作用。在皮肤过敏中,Th2 细胞和 TFH 细胞的分化和功能受皮肤衍生因子的影响,包括上皮细胞因子、趋化因子和信号通路,从而改变迁移性 DC 和传统 DC 的功能。在这篇综述中,我们旨在了解 DCs 参与过敏反应的具体机制,以便深入了解过敏性疾病的发病机制和潜在的治疗策略。
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引用次数: 0
The skin barrier and microbiome in infantile atopic dermatitis development: can skincare prevent onset? 婴儿特应性皮炎发育过程中的皮肤屏障和微生物群:皮肤护理能预防发病吗?
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-10-26 DOI: 10.1093/intimm/dxae038
Tomoka Ito, Yuumi Nakamura

Atopic dermatitis (AD), a prevalent Th2-dominant skin disease, involves complex genetic and environmental factors, including mutations in the Filaggrin gene and dysbiosis of skin microbiota characterized by an increased abundance of Staphylococcus aureus. Our recent findings emphasize the pivotal role of the skin barrier's integrity and microbial composition in infantile AD and allergic diseases. Early skin dysbiosis predisposes infants to AD, suggesting targeted skincare practices as a preventive strategy. The effects of skincare interventions, particularly the application of moisturizers with the appropriate molar concentration of ceramides, cholesterol, and fatty acids, play a crucial role in restoring the skin barrier. Notably, our study revealed that appropriate skincare can reduce Streptococcus abundance while supporting Cutibacterium acnes presence, thus directly linking skincare practices to microbial modulation in neonatal skin. Despite the mixed outcomes of previous Randomized Controlled Trials on the efficacy of moisturizers in AD prevention, our research points to the potential of skincare intervention as a primary preventive method against AD by minimizing the impact of genetic and environmental factors. Furthermore, our research supports the notion that early aggressive management of eczema may reduce the incidence of food allergies, highlighting the necessity for multifaceted prevention strategies that address both the skin barrier and immune sensitization. By focusing on repairing the skin barrier and adjusting the skin's microbiome from birth, we propose a novel perspective on preventing infantile AD and allergic diseases, opening new avenues for future studies, and practices in allergy prevention.

特应性皮炎(AD)是一种流行的 Th2 主导型皮肤病,涉及复杂的遗传和环境因素,包括 Filaggrin 基因突变和以金黄色葡萄球菌增多为特征的皮肤微生物群失调。我们最近的研究结果强调了皮肤屏障的完整性和微生物组成在婴儿 AD 和过敏性疾病中的关键作用。早期皮肤菌群失调容易导致婴儿过敏性鼻炎,这表明有针对性的护肤方法是一种预防策略。护肤干预的效果,尤其是使用含有适当摩尔浓度神经酰胺、胆固醇和脂肪酸的保湿剂,对恢复皮肤屏障起着至关重要的作用。值得注意的是,我们的研究表明,适当的护肤品可以减少链球菌的数量,同时支持痤疮棒状杆菌的存在,从而将护肤方法与新生儿皮肤的微生物调节直接联系起来。尽管之前的随机对照试验对保湿剂在预防AD方面的功效结果不一,但我们的研究表明,护肤干预可最大限度地减少遗传和环境因素的影响,从而有可能成为预防AD的主要方法。此外,我们的研究还支持这样一种观点,即早期积极治疗湿疹可降低食物过敏的发病率,这突出说明了同时解决皮肤屏障和免疫致敏问题的多方面预防策略的必要性。通过关注从出生开始修复皮肤屏障和调整皮肤的微生物群,我们提出了预防婴幼儿AD和过敏性疾病的新观点,为过敏预防的未来研究和实践开辟了新途径。
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引用次数: 0
Dysfunction of type 1 and type 2 immune cells: a lesson from exhausted-like ILC2s and their activation-induced cell death. 1 型和 2 型免疫细胞的功能失调:从枯竭样 ILC2 及其活化诱导的细胞死亡中汲取教训。
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-10-26 DOI: 10.1093/intimm/dxae032
Takashi Ebihara, Toshiki Yamada, Akane Fuchimukai, Shunsuke Takasuga, Tentaro Endo, Takechiyo Yamada, Megumi Tatematsu

The concept of immune cell exhaustion/dysfunction has developed mainly to understand impaired type 1 immune responses, especially by CD8 T-cells against tumors or virus-infected cells, and has been applied to other lymphocytes. Natural killer (NK) cells and CD4 T cells support the efficient activation of CD8 T cells but exhibit dysfunctional phenotypes in tumor microenvironments and in chronic viral infections. In contrast, the concept of type 2 immune cell exhaustion/dysfunction is poorly established. Group 2 innate lymphoid cells (ILC2s) and T-helper 2 (Th2) cells are the major lymphocyte subsets that initiate and expand type 2 immune responses for antiparasitic immunity or allergy. In mouse models of chronic parasitic worm infections, Th2 cells display impaired type 2 immune responses. Chronic airway allergy induces exhausted-like ILC2s that quickly fall into activation-induced cell death to suppress exaggerated inflammation. Thus, the modes of exhaustion/dysfunction are quite diverse and rely on the types of inflammation and the cells. In this review, we summarize current knowledge of lymphocyte exhaustion/dysfunction in the context of type 1 and type 2 immune responses and discuss ILC2-specific regulatory mechanisms during chronic allergy.

免疫细胞衰竭/功能障碍的概念主要是为了理解受损的第一类免疫反应,尤其是 CD8 T 细胞对肿瘤或病毒感染细胞的反应,这一概念也被应用于其他淋巴细胞。自然杀伤(NK)细胞和 CD4 T 细胞支持 CD8 T 细胞的有效激活,但在肿瘤微环境和慢性病毒感染中表现出功能失调的表型。相比之下,第 2 类免疫细胞衰竭/功能失调的概念尚未确立。第 2 组先天性淋巴细胞(ILC2s)和 T 辅助细胞 2(Th2)是启动和扩大 2 型免疫反应的主要淋巴细胞亚群,可用于抗寄生虫免疫或过敏。在慢性寄生蠕虫感染的小鼠模型中,Th2 细胞显示出受损的 2 型免疫反应。慢性气道过敏会诱导类似衰竭的 ILC2,这些 ILC2 会迅速陷入活化诱导的细胞死亡,以抑制夸张的炎症反应。因此,衰竭/功能障碍的模式多种多样,取决于炎症类型和细胞。在这篇综述中,我们总结了目前在 1 型和 2 型免疫反应背景下有关淋巴细胞衰竭/功能障碍的知识,并讨论了慢性过敏过程中 ILC2 的特异性调节机制。
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引用次数: 0
Ro5-4864, a translocator protein ligand, regulates T cell-mediated inflammatory responses in skin. 转运蛋白配体 Ro5-4864 可调节 T 细胞介导的皮肤炎症反应。
IF 4.8 4区 医学 Q2 IMMUNOLOGY Pub Date : 2024-10-26 DOI: 10.1093/intimm/dxae065
Yuka Sendai, Kazuyoshi Takeda, Keisuke Ohta, Susumu Nakae, Kyotaro Koshika, Kei Kitamura, Makoto Higuchi, Tatsuya Ichinohe, Toshifumi Azuma, Ko Okumura, Tatsukuni Ohno

Translocator protein (TSPO) is a mitochondrial outer membrane protein expressed on a variety of immune cells, including macrophages, dendritic cells, and T cells, in addition to neurons and steroid-producing cells. Previous studies of TSPO ligands have suggested that TSPO is involved in multiple cellular functions, including steroidogenesis, immunomodulation, and cell proliferation. Currently, there are limited reports on the effects of TSPO or TSPO ligands on T cell-mediated immune responses. We here investigated the involvement of TSPO/TSPO ligand in T cell responses using a 2,4-dinitro-1-fluorobenzene (DNFB)-induced contact hypersensitivity (CH) model. Treatment with Ro5-4864, a TSPO ligand, during DNFB sensitization reduced the number and activation status of CD4+ and CD8+ T cells in draining lymph nodes and alleviated skin inflammation after DNFB challenge. Adoptive transfer of Ro5-4864-treated mouse-derived DNFB-sensitized T cells to naïve mice inhibited CH responses after DNFB challenge. Ro5-4864-treated sensitized T cells showed lower proliferative responses when stimulated with DNFB-pulsed antigen-presenting cells compared to control-treated sensitized T cells. Ro5-4864 also suppressed cell proliferation, as well as adenosine triphosphate and lactate production, during T cell activation. Moreover, the inhibitory effects of Ro5-4864 on T cell responses were conserved in TSPO-deficient cells. Our results suggest that Ro5-4864 inhibits CH responses by suppressing energy metabolism, at least via glycolysis, to reduce the T cell primary response in a TSPO-independent manner.

转运蛋白(TSPO)是一种线粒体外膜蛋白,表达于多种免疫细胞,包括巨噬细胞、树突状细胞和 T 细胞,以及神经元和类固醇生成细胞。以往对 TSPO 配体的研究表明,TSPO 参与多种细胞功能,包括类固醇生成、免疫调节和细胞增殖。目前,有关 TSPO 或 TSPO 配体对 T 细胞介导的免疫反应的影响的报道有限。我们在此使用 2,4-二硝基-1-氟苯(DNFB)诱导的接触过敏(CH)模型研究了 TSPO/TSPO 配体参与 T 细胞反应的情况。在DNFB致敏过程中使用TSPO配体Ro5-4864可减少引流淋巴结中CD4+和CD8+T细胞的数量和活化状态,并减轻DNFB挑战后的皮肤炎症。将经过 Ro5-4864 处理的小鼠 DNFB 致敏 T 细胞收养转移到幼稚小鼠体内可抑制 DNFB 挑战后的 CH 反应。与对照组相比,Ro5-4864处理过的致敏T细胞在受到DNFB脉冲抗原递呈细胞刺激时显示出较低的增殖反应。Ro5-4864 还能抑制 T 细胞活化过程中的细胞增殖以及三磷酸腺苷和乳酸的产生。此外,Ro5-4864 对 T 细胞反应的抑制作用在 TSPO 缺陷细胞中保持不变。我们的研究结果表明,Ro5-4864 通过抑制能量代谢(至少通过糖酵解)来抑制 CH 反应,从而以独立于 TSPO 的方式降低 T 细胞的初级反应。
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International immunology
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