纤维素合成酶样 OsCSLD4:水稻农艺性状、抗病性和代谢指数的关键调节因子。

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-10-16 DOI:10.1007/s00299-024-03356-y
Guofang Zhang, Zhuang Yang, Shen Zhou, Jinjin Zhu, Xianqing Liu, Jie Luo
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引用次数: 0

摘要

关键信息根据水稻多组学分析,纤维素合成酶样OsCSLD4在调控多种农艺性状、增强对细菌性叶枯病的抗性以及调节代谢物指数方面发挥着关键作用。为了深入研究水稻农艺性状与代谢物之间的复杂网络,我们编制了一个涵盖基因组、表型组和代谢组的数据集,其中包括 524 个不同品种、11 个农艺性状和 841 个代谢物,从而通过 GWAS 找出了 8 个热点。随后,我们发现了四个不同的代谢物类别,包括同时存在于QTL qC12.1上的15种代谢物,这些代谢物与旗叶角度和小穗长度相关,并最终聚焦于纤维素合成酶样OsCSLD4,通过包括序列变异、单体型、ATAC和在不同组织中的差异表达在内的严格过程将其准确定位。与野生型相比,csld4 的株高、旗叶长度、叶宽、穗长、千粒重、粒宽、粒厚、生育力、单株产量和抗细菌性枯萎病能力均显著降低。但是,分蘖数、卷叶程度、开花期、生长期、谷粒长度和空仁率都有明显增加。此外,在突变体 csld4 中,除代谢物 N-阿魏酰基酪胺(mr1268)外,其他四种多酚代谢物的含量明显上升,而其他三种多酚代谢物的含量,即 smiglaside C(mr1498)、4-香豆酸(mr1622)和 smiglaside A(mr1925)则明显下降。氨基酸 L-酪胺(mr1446)的含量在突变体中明显增加,而生物碱三尖杉碱(mr1188)的含量则大幅减少。这项研究从多组学角度全面分析了OsCSLD4的遗传机制,为育种者提供了提高水稻产量、抗细菌性叶枯病能力和代谢物含量的潜在途径,从而提高水稻生产的可持续性和盈利能力。
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Cellulose synthase-like OsCSLD4: a key regulator of agronomic traits, disease resistance, and metabolic indices in rice.

Key message: Cellulose synthase-like OsCSLD4 plays a pivotal role in regulating diverse agronomic traits, enhancing resistance against bacterial leaf blight, and modulating metabolite indices based on the multi-omics analysis in rice. To delve deeper into this complex network between agronomic traits and metabolites in rice, we have compiled a dataset encompassing genome, phenome, and metabolome, including 524 diverse accessions, 11 agronomic traits, and 841 metabolites, enabling us to pinpoint eight hotspots through GWAS. We later discovered four distinct metabolite categories, encompassing 15 metabolites that are concurrently present on the QTL qC12.1, associated with leaf angle of flag and spikelet length, and finally focused the cellulose synthase-like OsCSLD4, which was pinpointed through a rigorous process encompassing sequence variation, haplotype, ATAC, and differential expression across diverse tissues. Compared to the wild type, csld4 exhibited significant reductions in the plant height, flag leaf length, leaf width, spikelet length, 1000-grain weight, grain width, grain thickness, fertility, yield per plant, and bacterial blight resistance. However, there were significant increase in tiller numbers, degree of leaf rolling, flowering period, growth period, grain length, and empty kernel rate. Furthermore, the content of four polyphenol metabolites, excluding metabolite N-feruloyltyramine (mr1268), notably rose, whereas the levels of the other three polyphenol metabolites, smiglaside C (mr1498), 4-coumaric acid (mr1622), and smiglaside A (mr1925) decreased significantly in mutant csld4. The content of amino acid L-tyramine (mr1446) exhibited a notable increase, whereas the alkaloid trigonelline (mr1188) displayed a substantial decrease among the mutants. This study offered a comprehensive multi-omics perspective to analyze the genetic mechanism of OsCSLD4, and breeders can potentially enhance rice's yield, bacterial leaf blight resistance, and metabolite content, leading to more sustainable and profitable rice production.

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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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