溶乳假杆菌产生的正丁酸使 Epstein-Barr 病毒重新活化,诱导根尖周炎肉芽肿中的炎性细胞因子。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Biosciences Pub Date : 2025-03-01 DOI:10.1016/j.job.2024.10.001
Taiki Miyata , Osamu Takeichi , Kenichi Imai , Masayuki Okano , Seiya Inoue , Takuya Yasukawa , Yusuke Suzuki
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引用次数: 0

摘要

研究目的本研究探讨了潜伏的爱泼斯坦-巴氏病毒(EBV)能否被溶乳假杆菌的正丁酸重新激活,以及这种重新激活能否诱导根尖周炎肉芽肿中白细胞介素(IL)-1β和IL-6的表达:我们分析了根尖周炎肉芽肿和健康牙龈组织,以检测是否存在 EBV 和溶乳杆菌。测量溶乳杆菌培养上清液中的正丁酸浓度。用或不用这些上清液进行 BZLF-1 荧光素酶检测。对组织样本中的 ZEBRA-、IL-1β 和 IL-6 表达细胞进行免疫组化检测。此外,还对 BZLF-1、IL-1β 和 IL-6 的 mRNA 表达水平进行了量化和相关性统计分析。在使用或不使用培养上清液处理的 Daudi 细胞中也检测了这些 mRNA 的表达:结果:在根尖周炎肉芽肿中检测到 EBV 和溶乳杆菌,而在健康组织中未检测到。培养上清液中的正丁酸浓度为 3.58 mmol/L。在有培养上清存在的情况下,BZLF-1荧光素酶的活性与市售丁酸的活性相当,而在没有培养上清的情况下则检测不到任何活性。表达 ZEBRA 的细胞同时表达 IL-1β 和 IL-6。根尖周炎肉芽肿中 BZLF-1、IL-1β 和 IL-6 的 mRNA 水平与 EBV DNA 拷贝数相关。用培养上清液处理的 Daudi 细胞表达 BZLF-1、IL-1β 和 IL-6 mRNA,而未用培养上清液处理的细胞则不表达:本研究得出结论:EBV 可被溶乳杆菌产生的正丁酸重新激活,从而诱导根尖周炎肉芽肿中 IL-1β 和 IL-6 的表达。
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Reactivation of Epstein-Barr virus by n-butyric acid from Pseudoramibacter alactolyticus induces inflammatory cytokines in periapical granulomas

Objectives

This study investigates whether latent Epstein-Barr virus (EBV) can be reactivated by n-butyric acid from Pseudoramibacter alactolyticus, and if such reactivation induces expression of interleukin (IL)-1β and IL-6 in periapical granulomas.

Methods

We analyzed periapical granulomas and healthy gingival tissues to detect the presence of EBV and P. alactolyticus. The concentration of n-butyric acid in P. alactolyticus culture supernatants was measured. BZLF-1 luciferase assays were conducted with or without these supernatants. Immunohistochemical detection of ZEBRA-, IL-1β-, and IL-6-expressing cells was performed in the tissue samples. Additionally, mRNA expression levels of BZLF-1, IL-1β, and IL-6 were quantified and statistically analyzed for correlation. The expression of these mRNAs was also measured in Daudi cells treated with or without the culture supernatants.

Results

Both EBV and P. alactolyticus were detected in periapical granulomas, but not in healthy tissues. The concentration of n-butyric acid in the culture supernatants was ∼3.58 mmol/L. BZLF-1 luciferase activity in the presence of the culture supernatants was comparable to that of commercially available butyric acid, whereas no activity was detected without the supernatants. Cells expressing ZEBRA co-expressed IL-1β and IL-6. The mRNA levels of IL-1β and IL-6 in periapical granulomas were correlated with BZLF-1 mRNA levels. Daudi cells treated with the culture supernatants expressed BZLF-1, IL-1β, and IL-6 mRNA, while those without the supernatants did not.

Conclusions

The study concludes that EBV can be reactivated by n-butyric acid produced by P. alactolyticus, leading to the induction of IL-1β and IL-6 expression in periapical granulomas.
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来源期刊
Journal of Oral Biosciences
Journal of Oral Biosciences DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
4.40
自引率
12.50%
发文量
57
审稿时长
37 days
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